A Review on Role of Enzymes and Microbes in Healing Cracks in Cementitious Materials

Fang, Chaolin; Płaza, Grażyna; Achal, Varenyam

doi:10.1007/978-981-16-1706-5_9

Cited by 4 publications

(3 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When pH and DIC increase in an environment with abundant Ca 2+ ions, bacterial cell walls and/or their EPS can serve as a nucleation site for the precipitation reaction [4] , [10] , [11] . Other mechanisms involved in BCP are the precipitation of carbonates by ion exchange across the cell membrane [5] or the effect of carbonic anhydrase (CA) [12] . Following these principles, different bacterially mediated mineralization processes have been distinguished, depending on whether they are influenced by extracellular organic molecules, induced by metabolic activity or controlled by specific genes [9] .…”

Section: Introductionmentioning

confidence: 99%

“…The ecological implications of the BCP are varied. Its ecological relevance has been exploited in biotechnology, with applications in the bioremediation of metal-contaminated soils and groundwater, soil and sand bio-consolidation, cement and CO 2 sequestration, among others [3] , [12] , [35] – [39] . Although CaCO 3 biomineralization has been studied due to its wide range of scientific and technological implications, the essential role and molecular mechanisms of this crucial geomicrobiological process are largely unknown.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preliminary indication of the role of AHL-dependent quorum sensing systems in calcium carbonate precipitation in Gram-negative bacteria

Cacchio,

Pellegrini,

Farda

et al. 2023

AIMSMICRO

View full text Add to dashboard Cite

<abstract> Numerous microbial species participate in precipitation of carbonates in various natural environments, including soils, geological formations, freshwater biofilms and oceans. Despite the geochemical interest of such a biomineralization process, its molecular mechanisms and adaptive aspects remain poorly known. Many Gram-negative bacteria use cell-to-cell communication systems relying on N-acylhomoserine lactone (AHLs) signal molecules to express certain phenotypic traits in a density-dependent manner, a phenomenon referred as to quorum-sensing (QS). In this work, bacterial isolates collected from cave and rhizosphere soil were analyzed to study the occurrence of the AHL-mediated QS in bacterial calcium carbonate (CaCO3) precipitation. To test the production of AHLs signal molecules, we cross-streaked Gram-negative calcifying strains, selected among the environmental strains studied, with the AHL-negative mutant <italic>Chromobacterium subtsugae</italic> strain CV026. Only <italic>Burkholderia ambifaria</italic> LMG 11351 was able to restore violacein production in CV026 among the tested strains. The constructed AHL-negative mutant of <italic>B. ambifaria</italic> LMG 11351 could not precipitate CaCO3 on B-4 agar. Scanning Electron Microscopy (SEM) analysis on CaCO3 crystals obtained <italic>in vitro</italic> shows crystals of different morphologies, calcified biofilms and bacteria in close contact with the precipitated crystals. In the inner layers of the bioliths deposited by <italic>B. ambifaria</italic> LMG 11351, a stream-like organization of the <italic>Burkholderia</italic> imprints was not detected by SEM. Our data provide preliminary evidence that the activation of AHL-regulated genes may be a prerequisite for <italic>in vitro</italic> bacterial carbonatogenesis, in some cases, confirming the specific role of bacteria as CaCO3 precipitating agents. We enhance the understanding of bacterial CaCO3 biomineralization and its potential biotechnology implications for QS-based strategies to enhance or decrease CaCO3 precipitation through specific bacterial processes. The AHL-negative mutant of <italic>B. ambifaria</italic> LMG 11351 (a well-known plant growth-promoting bacterium) could also be used to study plant-bacteria interactions. The adaptive role of bacterial CaCO3 biomineralization was also discussed. </abstract>

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preliminary indication of the role of AHL-dependent quorum sensing systems in calcium carbonate precipitation in Gram-negative bacteria

Cacchio,

Pellegrini,

Farda

et al. 2023

AIMSMICRO

View full text Add to dashboard Cite

show abstract

“…The precipitation of carbonates is governed mainly by four factors: (a) calcium concentration, (b) carbonate concentration, (c) pH of the environment, and (d) presence of nucleation sites (Hammes & Verstraete, 2002). CaCO 3 precipitation is a biological and a geochemical method of producing carbonates of calcium with the help of microorganisms, especially those residing inside cave soil (Fang et al, 2021; Mortensen et al, 2011). Many bacterial cell walls are covered with organic layers.…”

Section: Introductionmentioning

confidence: 99%

Calcium carbonate precipitation by cave bacteria isolated from Kashmir Cave, Khyber Pakhtunkhwa, Pakistan

Jan

Zada

Rafiq

et al. 2022

Microscopy Res & Technique

View full text Add to dashboard Cite

The participation of numerous physicochemical and biological functions maintains the evolution and expansion of the remarkable nature. Due to its vast applicability in several engineering disciplines, naturally occurring bio-mineralization or microbially induced calcium carbonate (MICP) precipitation is attracting more interest. Cave bacteria contribute to the precipitation of calcium carbonate (CaCO 3 ). In the present study, soil sediments were collected from Kashmir cave, KPK, Pakistan, and plated on B4 specific nutrients limited medium for bacterial isolation and the viable bacterial count was calculated. Three bacterial strains named were capable of precipitating CaCO 3 . These bacterial isolates were identified through 16S rRNA gene sequencing and strain GSN-11 was identified as Bacillus toyonensis, TFSN-14 as Paracoccus limosus and TFSN-15 as Brevundimonas diminuta. Enhanced CaCO 3 precipitation potential of these bacteria strains was observed at 25 C and pH 5. The precipitated CaCO 3 was confirmed by scanning electron microscopy, X-ray powder diffraction, and Fourier transform infra-red spectroscopy. The findings showed that the precipitates were dominated by calcite, aragonite, and nanosize vaterite. Current research suggests that precipitation of CaCO 3 by proteolytic cave bacteria is widespread in Kashmir cave and these bacterial communities can actively contribute to the formation of CaCO 3 by enhancing the pH of the microenvironment. Research Highlights• Kashmir cave inhabit potentially active bacteria in terms of biogeochemical processes.• Cave bacteria significantly precipitated CaCO 3 .• Calcite, aragonite, and nanosize vaterite were dominant in precipitates.

show abstract

Self-healing concrete for sustainable buildings. A review

et al. 2022

View full text Add to dashboard Cite

A Review on Role of Enzymes and Microbes in Healing Cracks in Cementitious Materials

Cited by 4 publications

References 52 publications

Preliminary indication of the role of AHL-dependent quorum sensing systems in calcium carbonate precipitation in Gram-negative bacteria

Preliminary indication of the role of AHL-dependent quorum sensing systems in calcium carbonate precipitation in Gram-negative bacteria

Calcium carbonate precipitation by cave bacteria isolated from Kashmir Cave, Khyber Pakhtunkhwa, Pakistan

Self-healing concrete for sustainable buildings. A review

Contact Info

Product

Resources

About