A Review on Spike and Recovery Method in Analytical Method Development and Validation

Thakur, Diksha; Dubey, Neeti Prakash; Singh, Rajvinder

doi:10.1080/10408347.2022.2152275

Cited by 11 publications

(4 citation statements)

References 84 publications

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“…Under optimised analytical conditions, an application study has been conducted in synthetic physiological fluids for determining ST and Tryp using pGlu-AgNPs/GCE following the spike recovery analysis (Table II). 20 Good recovery and RSD values suggest that pGlu-AgNPS/GCE can be successfully applied for the electrochemical sensing of ST and Tryp.…”

Section: Effect Of Number Of Cycles Of Electrodeposition Of Pglu-agnps-mentioning

confidence: 96%

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Simultaneous Determination of Serotonin and Tryptamine Using Polyglutamic Acid-Silver Nanoparticles Composite Film Modified Glassy Carbon Electrode

Swathy,

Kumar

2023

J. Electrochem. Soc.

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Serotonin (ST), chemically 5-hydroxy tryptamine, is a neurotransmitter which mediates a series of central and peripheral functions in the human body and has a crucial role in gastrointestinal and psychiatric disorders. Tryptamine (Tryp) is also a neurotransmitter, mainly found in protein-rich foods, beverages and other fermented foods. The accumulation of higher concentrations of Tryp is harmful to human health. So, it is important to determine both ST and Tryp in our body. A polyglutamic acid (pGlu)-Silver Nanoparticles (AgNPs) composite film modified Glassy Carbon Electrode (pGlu-AgNPs/GCE) has been developed for the simultaneous determination of ST and Tryp. The incorporation of AgNPs with pGlu makes the sensor more efficient and reliable for the voltammetric sensing of ST and Tryp. Experimental parameters were optimised and, the sensor showed a linear concentration range for the determination of ST and Tryp from 1.00 × 10–5 M to 7.00 × 10–7 M and 1.00 × 10–5 M to 9.00 × 10–7 M, respectively. The electrooxidation of ST at pGlu-AgNPs/GCE was found to be diffusion controlled, whereas adsorption controlled electrooxidation was found for Tryp. The applicability of the sensor in determining ST and Tryp has been checked in artificial physiological fluids.

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Section: Effect Of Number Of Cycles Of Electrodeposition Of Pglu-agnps-mentioning

confidence: 96%

“…The reliability of the sensor has been checked by the spike recovery analysis of ST and Tryp in artificial urine and blood serum. 20 Prepared solutions were spiked with ST and Tryp, and further measurements were conducted using SWV.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Simultaneous Determination of Serotonin and Tryptamine Using Polyglutamic Acid-Silver Nanoparticles Composite Film Modified Glassy Carbon Electrode

Swathy,

Kumar

2023

J. Electrochem. Soc.

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“…31 In recovery trials, the analytical findings for recovered materials at LQC, MQC, and HQC must be compared with un-extracted standards, which indicate 100% recovery. 32 • Lower limit of quantification The Lower limit of quantification (LLoQ) is a minimal analyte quantity at which measurements may be made with respectable accuracy and precision. If the requirements listed below are satisfied, the minimum standard on the calibration graph must be considered as LLoQ.…”

Section: • Recoverymentioning

confidence: 99%

Analytical Review on Newly Approved Antidiabetic and Antihypertensive Drugs

Pravalika,

2024

IJDDT

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Diabetes and hypertension are the principal causes of death all over the world. Both diabetes and hypertension coexist frequently. Managing both is a multi-faced task requiring extensive care. Therapeutic intervention optimization and ensuring patient safety are the results of assessing medications in biological samples. The development of an analytical technique specific to the measurement of a medicinal chemical in a certain matrix requires careful consideration of the various equipment options and an understanding of their relative capabilities with regard to selectivity, sensitivity, usability, speed of investigation, etc. This article aimed to systematically analyze many popular quantitative analytical techniques for measuring some newly approved antidiabetic and antihypertensive drugs in pharmaceutical planning. Several chromatographic techniques have been employed for quantitative research into these pharmaceuticals. Rapid, accurate, cost-effective, and straightforward drug analysis methods are now essential for developing and using new technologies.

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“…As part of the quality assessment of the extraction procedure apart from extraction recovery, spike recovery, or recovery of known addition, was also performed. This technique is based on the addition of a known concentration of analyte to the matrix which is either similar to or identical to the sample [26]. As phthalates occur ubiquitously in our surrounding environment as well as in sand and sediments, it was difficult to obtain a sand sample which completely lacked DBP and DEHP.…”

Section: Preliminary Extraction Studiesmentioning

confidence: 99%

Presence of Phthalate Esters Used as Common Plasticisers in Maltese Shoreline Sand

2023

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DBP and DEHP are two of the most common plasticisers used in modern industries, which have aroused a major concern over their ubiquitous occurrence and hazardous environmental effects. This study presents the first data of phthalates occurrence and distribution in Maltese shoreline sand. DBP and DEHP occurrence was investigated in three busy and secluded beaches, respectively. Analytical determination involved quantifying DBP and DEHP from 75 samples using UPLC–MS/MS through triple repeatability. The concentrations ranged between 0.0095 and 0.07034 µg/g and <LOQ and 0.0977 µg/g for DBP and DEHP, respectively. Statistical analysis of the comparison of phthalates in different beaches demonstrated that the occurrence of DBP and DEHP in Maltese shoreline sand varies. The differences in the occurrence of phthalates were attributed to the beach topography and anthropogenic pressures. Analysis of the effects of anthropogenic activities on the occurrence of phthalates showed a positive association. Distribution analysis showed that DBP and DEHP exhibit an uneven distribution along the shoreline. Conversely, different distributions were observed at distances away from the sea. The uneven distributions observed were associated with anthropogenic activities, different chemodynamic properties and wave action. Cluster analysis was performed to prove the interactions between the phthalates concentrations and the characteristics of the beaches.

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A Review on Spike and Recovery Method in Analytical Method Development and Validation

Cited by 11 publications

References 84 publications

Simultaneous Determination of Serotonin and Tryptamine Using Polyglutamic Acid-Silver Nanoparticles Composite Film Modified Glassy Carbon Electrode

Simultaneous Determination of Serotonin and Tryptamine Using Polyglutamic Acid-Silver Nanoparticles Composite Film Modified Glassy Carbon Electrode

Analytical Review on Newly Approved Antidiabetic and Antihypertensive Drugs

Presence of Phthalate Esters Used as Common Plasticisers in Maltese Shoreline Sand

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