A review on surfactin: molecular regulation of biosynthesis

Qi, Xiaohua; Liu, Wei; He, Xin; Du, Chunmei

doi:10.1007/s00203-023-03652-3

Cited by 6 publications

(5 citation statements)

References 124 publications

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“…In contrast, Yaseen et al [ 64 ] revealed that the knockout of srfAC did not significantly affect fengycin production, while the deletion of srfAA resulted in a significant decrease. It is hypothesized that the reason for this phenomenon may be due to the inclusion of comS , the gene encoding an anti-adaptive protein that protects the regulatory factor ComK from post-translational degradation [ 95 ], in the open reading frame of srfAA . In a later study, Vahidinasab et al [ 67 ] reintroduced comS into the genome after deleting the entire srfAA-AD gene cluster in B. subtilis BMV12 and BMV13.…”

Section: Enhancing Fengycin Production Through Metabolic Engineeringmentioning

confidence: 99%

“…Then the phosphoryl group is transferred to response regulator ComA, and the phosphorylated ComA binds to a specific locus to regulate the transcription and expression of corresponding genes (Fig. 3 ) [ 79 , 95 , 98 ]. It has been shown that the knockout of comA in B. amyloliquefaciens fmbJ resulted in a significant decrease in fengycin yield, while the overexpression of comA led to a 2.6-fold increase [ 80 ], indicating that ComA is an important regulator in fengycin synthesis.…”

Section: Enhancing Fengycin Production Through Metabolic Engineeringmentioning

confidence: 99%

“…The knockout of comA resulted in a significant down-regulation of the expression of degU [ 80 ], indicating that ComA positively regulates the DegS-DegU two-component regulatory system. It is hypothesized that this regulatory effect may be mediated by DegQ, since ComP-ComA positively regulates the expression of degQ [ 95 , 99 ]. Additionally, previous studies have shown that the yield of fengycin significantly decreased in strains with degU knockout [ 80 , 81 ], and the expression level of fenA was also down-regulated [ 81 ].…”

Section: Enhancing Fengycin Production Through Metabolic Engineeringmentioning

confidence: 99%

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Strategies for improving fengycin production: a review

Yin,

Wang,

Zhang

et al. 2024

Microb Cell Fact

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Fengycin is an important member of the lipopeptide family with a wide range of applications in the agricultural, food, medical and cosmetic industries. However, its commercial application is severely hindered by low productivity and high cost. Therefore, numerous studies have been devoted to improving the production of fengycin. We summarize these studies in this review with the aim of providing a reference and guidance for future researchers. This review begins with an overview of the synthesis mechanism of fengycin via the non-ribosomal peptide synthetases (NRPS), and then delves into the strategies for improving the fengycin production in recent years. These strategies mainly include fermentation optimization and metabolic engineering, and the metabolic engineering encompasses enhancement of precursor supply, application of regulatory factors, promoter engineering, and application of genome-engineering (genome shuffling and genome-scale metabolic network model). Finally, we conclude this review with a prospect of fengycin production.

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Section: Enhancing Fengycin Production Through Metabolic Engineeringmentioning

confidence: 99%

Section: Enhancing Fengycin Production Through Metabolic Engineeringmentioning

confidence: 99%

Section: Enhancing Fengycin Production Through Metabolic Engineeringmentioning

confidence: 99%

See 1 more Smart Citation

Strategies for improving fengycin production: a review

Yin,

Wang,

Zhang

et al. 2024

Microb Cell Fact

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“…This study is the rst to demonstrate that space ight had a pronounced impact on secondary metabolism process of lipopeptide production and to elucidate the mechanism underlying the decrease in surfactin production based on comparative transcriptomic analysis. Surfactin synthesis was accomplished by non-ribosomal peptide synthetase (NRPSs), which mainly involves three steps: the synthesis of fatty acid chain, the activation and loading of fatty acids, and the extension of the peptide chain [51]. Fatty acid synthesis plays as a critical role in lipopeptide synthesis and the gene expression levels are positively correlated with the lipopeptide yield [52,53].…”

Section: Metabolic Pathways and Regulatory Mechanisms Response For Sp...mentioning

confidence: 99%

The metabolic and physiological responses to spaceflight of a lipopeptide- producing Bacillus subtilis

Qin,

Liu,

Liu

et al. 2024

Preprint

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Background The outer space is an extreme environment that has attracted continuous interest in microbial adaptation and safety, due to its high mutagenesis frequency and genetic variability. To date, several studies have assessed the impact of the space environment on the microbiomes and microorganisms. While the survival of Bacillus subtilis after spaceflight is well established, how the phenotype and metabolic function of B. subtilis respond to space stress is rarely reported. Results In this study, we performed a space flight of the B. subtilis TD7 strain facilitated by the launch project of the Xinyidai Zairen Feichuan-Shiyan Chuan, and compared the strains after spaceflight with the wild-type in terms of their growth, morphology, biofilm formation and secondary metabolism. The spaceflight strain exhibited slower growth, higher cell density, different morphology and decreased biofilm formation. Importantly, a decrease in the lipopeptide production was observed after spaceflight. Thus, we used a multi-omics approach to uncover the molecular mechanisms underlying the changeable secondary metabolism. A total of 14 gene clusters for secondary metabolite biosynthesis were identified in both the wild-type strain and spaceflight strains through whole-genome sequencing, including nonribosomal peptide synthetase. The comparative transcriptome revealed 997 differentially expressed genes which involved in the TCA cycle, fatty acid degradation, amino acid biosynthesis, and quorum sensing systems. The differential expression analysis of 26 lipopeptide-related DEGs further elucidated the relationship between the space environment and the regulation of secondary metabolism. Conclusion Our study is the first study to provide new insight into the behaviors, metabolic functions and adaptation mechanisms of B. subtilis in response to spaceflight. This knowledge could contribute to a better understanding of the relationship between the space environment and microbial adaption mechanisms.

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“…Surfactin, a highly effective biosurfactant produced by Bacillus subtilis strains, was proven with great biodegradability and low toxicity, which can be safely applied to cosmetics [16][17][18][19]. It has also been found as a pharmacologically active substance, such as antibacterial and antifungal, antimycoplasma, antiviral, anti-in ammatory, and thrombolytic properties [20][21][22][23][24][25][26][27]. Additionally, the emulsifying activity of surfactin makes it becoming an attractive candidate for MEs because its amphiphilic structure can form a uniform and stable dispersion.…”

Section: Introductionmentioning

confidence: 99%

Microemulsions constructed by a biosurfactant

Han,

Ma,

Zhang

et al. 2024

Preprint

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Due to their biodegradability and low toxicity, biosurfactants have been recently attracted much attention. Here, microemulsions (MEs) are constructed by an excellent biosurfactant, surfactin, via low energy emulsification. Rheological properties, kinetic stability, thermodynamic stability, and long-term storage capacity of microemulsions were characterized. The effect of pH and different gelling agents on the phase behavior and the formation of microemulsion-gels was also explored. Results reveal that the MEs show no significant change in droplet size under high-speed centrifugation, providing great kinetic stability, which enables the MEs to maintain their stability under bumpy and rugged transportation conditions. The MEs are thermodynamically stable and can be restored to their original state after experiencing extreme conditions such as high temperature (58℃) or freeze-thawing cycles, facilitating the use of the MEs under extreme conditions and promoting the shelf-life of production. The addition of different gelling agents did not show any significant changes to MEs and not disrupt the formation of the MEs, meaning that the MEs constructed by surfactin are stable and possess antioxidant potential and moisturizing effects.

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A review on surfactin: molecular regulation of biosynthesis

Cited by 6 publications

References 124 publications

Strategies for improving fengycin production: a review

Strategies for improving fengycin production: a review

The metabolic and physiological responses to spaceflight of a lipopeptide- producing Bacillus subtilis

Microemulsions constructed by a biosurfactant

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