A review on the “in vitro” culture of freshwater mussels (Unionoida)

Lima, Priscila Machado Vieira; Lopes‐Lima, Manuel; Kovitvadhi, Uthaiwan; Kovitvadhi, Satit; Owen, Christopher T.; Machado, Jorge

doi:10.1007/s10750-012-1078-0

Cited by 32 publications

(31 citation statements)

References 43 publications

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“…The US Environmental Protection Agency (USEPA) currently considers unionid sensitivity data from tests meeting the ASTM International data quality objectives when setting national water quality criteria (US Environmental Protection Agency 2013). Rearing mussels using a host fish (in vivo propagation) provides a means to study mussel-host transformation efficiency and other interactions (Fritts et al 2013;Douda 2015), and this method typically produces abundant, high-quality juveniles (Lima et al 2012)-an important prerequisite for use in toxicity testing. Rearing mussels using a host fish (in vivo propagation) provides a means to study mussel-host transformation efficiency and other interactions (Fritts et al 2013;Douda 2015), and this method typically produces abundant, high-quality juveniles (Lima et al 2012)-an important prerequisite for use in toxicity testing.…”

Section: Introductionmentioning

confidence: 99%

“…Because unionid mussels have an obligate parasitic life stage on fish (Lefevre and Curtis 1912;Kat 1983;Fritts et al 2013), rearing mussels in a laboratory requires the maintenance of appropriate host fish for the duration of the transformation from larvae (glochidia) to juvenile. It is labor intensive, requiring the housing and care of host fish if the host species are known, and visual monitoring to collect transformed juveniles (Lima et al 2012). However, in vivo culture presents several challenges.…”

Section: Introductionmentioning

confidence: 99%

“…Rearing mussels using a host fish (in vivo propagation) provides a means to study mussel-host transformation efficiency and other interactions (Fritts et al 2013;Douda 2015), and this method typically produces abundant, high-quality juveniles (Lima et al 2012)-an important prerequisite for use in toxicity testing. Transformation success is often poor and highly variable, surviving juveniles come from a nonsterile environment, and they may be contaminated with xenobiotics from the host fish (Lima et al 2012). It is labor intensive, requiring the housing and care of host fish if the host species are known, and visual monitoring to collect transformed juveniles (Lima et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The glochidia complete the transformation process in the culture media, from which they derive protein and other nutrients, rather than from the host fish tissues (Isom and Hudson 1982;Dimock and Wright 1993;Lima et al 2012). Glochidia are placed into Petri dishes or flasks containing culture media supplemented with animal serum (often rabbit or horse).…”

Section: Introductionmentioning

confidence: 99%

“…Glochidia are placed into Petri dishes or flasks containing culture media supplemented with animal serum (often rabbit or horse). However, in vitro propagation requires specialized cell culture equipment, and there is risk of fungal or bacterial contamination of the culture media that can limit culture success (Owen et al 2010;Lima et al 2012). Culture techniques have greatly improved in recent years (Owen et al 2010), and >40 species have been successfully transformed in culture media (Lima et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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A Comparison of the chemical sensitivities between in vitro and in vivo propagated juvenile freshwater mussels: Implications for standard toxicity testing

Popp

Cope

McGregor

et al. 2018

Environmental Toxicology and Chemistry

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Unionid mussels are ecologically important and are globally imperiled. Toxicants contribute to mussel declines, and toxicity tests using juvenile mussels-a sensitive life stage-are valuable in determining thresholds used to set water quality criteria. In vitro culture methods provide an efficient way to propagate juveniles for toxicity testing, but their relative chemical sensitivity compared with in vivo propagated juveniles is unknown. Current testing guidelines caution against using in vitro cultured juveniles until this sensitivity is described. Our objective was to evaluate the relative sensitivity of juvenile mussels produced from both in vitro and in vivo propagation methods to selected chemicals. We conducted 96-h acute toxicity tests according to ASTM International guidelines with 3 mussel species and 6 toxicants: chloride, nickel, ammonia, and 3 copper-based compounds. Statistically significant differences between in vitro and in vivo juvenile 96-h median effect concentrations were observed in 8 of 17 tests, and in vitro juveniles were more sensitive in 6 of the 8 significant differences. At 96 h, 4 of the 8 statistically different tests for a given chemical were within a factor of 2, which is the intralaboratory variation demonstrated in a recent evaluation of mussel toxicity tests. We found that although differences in chemical sensitivity exist between in vitro and in vivo propagated juvenile mussels, they are within normal toxicity test variation. Therefore, in vitro propagated juvenile mussels may be appropriate for use in ASTM International-based toxicity testing. Environ Toxicol Chem 2018;37:3077-3085. © 2018 SETAC.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Comparison of the chemical sensitivities between in vitro and in vivo propagated juvenile freshwater mussels: Implications for standard toxicity testing

Popp

Cope

McGregor

et al. 2018

Environmental Toxicology and Chemistry

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The first millimetre – rearing juvenile freshwater pearl mussels (Margaritifera margaritifera L.) in plastic boxes

Eybe

Thielen²,

Bohn

et al. 2013

Aquatic Conservation

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ABSTRACT1. The last remaining population of Margaritifera margaritifera in the Our River (Luxembourg, Europe) has come close to extinction. It consists predominantly of adult animals and will disappear within a few years without assistance. Juvenile mussels cannot survive in the river bed interstices, which are clogged by fine sediments, and rearing methods are needed to help them through this critical period.2. The objectives of this study were to elucidate the best rearing conditions for juvenile mussels with respect to the food type and density of individuals in order to breed them in the laboratory to a size at which survival in the river is likely.3. Different food mixtures (combinations of algae, detritus and crushed red bloodworms) were fed to juvenile mussels in plastic boxes containing 500 mL of river water during a period of 110 days. To understand fluctuations in the concentrations of nitrate, nitrite and ammonium between water exchanges, these ions were analysed over a period of 8 days.4. The best rearing results were achieved with a combination of detritus and algae. This treatment resulted in a growth rate of 189% (up to 1.13 mm (SD ±0.30) per box) with a survival rate of 80% (101 dead mussels (SD ±163.71) per box). The optimum number of mussels per box was 200. When detritus was added to the boxes, levels of nitrite and ammonium were reduced by more than 50% compared with the initial value within 8 days. Without detritus, ion concentrations increased noticeably (ammonium >50%, nitrite >150%), probably explaining higher mortality rates.5. Juvenile mussels have food reserves for less than 8 days after excystment and need additional food as soon as possible after dropping from the host fish.6. Juvenile mussels showed the most favourable survival rate and growth rate if fed with a mixture of algae and detritus. Detritus functions not only as a food source but also as a biologically active compound which reduces harmful ions such as ammonium and nitrite in the boxes.

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Imperilled species in aquatic ecosystems: emerging threats, management and future prognoses

Gangloff

Edgar

Wilson

2016

Aquatic Conservation

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1. Earth's aquatic habitats are some of its most important ecosystems and support populations of many of its most imperilled species. The future demands of Earth's growing human populations will increasingly stress its aquatic resources on multiple spatial and temporal scales, as highlighted by numerous authors when predicting trends in biodiversity in many key aquatic hotspots.2. Identifying key stressors and understanding potential linkages between these stressors are important first steps to mitigating human impacts on at-risk aquatic resources. Here, an overview is provided of the established and emerging threats to aquatic biodiversity at local, regional and global scales, including emerging diseases, expanding influence of invasive species, new industries, and the accelerating trajectory of climate change, as well as perspectives on potential management strategies.

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A review on the “in vitro” culture of freshwater mussels (Unionoida)

Cited by 32 publications

References 43 publications

A Comparison of the chemical sensitivities between in vitro and in vivo propagated juvenile freshwater mussels: Implications for standard toxicity testing

A Comparison of the chemical sensitivities between in vitro and in vivo propagated juvenile freshwater mussels: Implications for standard toxicity testing

The first millimetre – rearing juvenile freshwater pearl mussels (Margaritifera margaritifera L.) in plastic boxes

Imperilled species in aquatic ecosystems: emerging threats, management and future prognoses

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