A review on the laccase assisted decolourization of dyes: Recent trends and research progress

Jeyabalan, Jothika; Veluchamy, Ajithkumar; V, Vishnu Priyan; Kumar, Ajit; Chandrasekar, Ragavan; Narayanasamy, Selvaraju

doi:10.1016/j.jtice.2023.105081

Cited by 29 publications

(6 citation statements)

References 115 publications

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“…The problem is further enhanced by the fact that 80% of the total discharge from the industries goes into the water stream. The development of efficient dye decolorization methods is, thus, a crucial issue, and this is a topic where WRF and laccases could play an important role [84]. However, the number of attempts at using cells of Pycnoporus spp.…”

Section: Decolorization and Degradation Of Synthetic Dyes By Pycnopor...mentioning

confidence: 99%

Biotransformation of Pollutants by Pycnoporus spp. in Submerged and Solid-State Fermentation: Mechanisms, Achievements, and Perspectives

Cheute,

Uber,

dos Santos

et al. 2024

Biomass

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Substantial amounts of organo-pollutants, often persistent and toxic, are generated globally each year, posing a threat to soil, water, groundwater, and air. The pollutants encompass a wide range of substances from various sources, which include solid as well as liquid ones, such as landfill leachates and wastewaters. The compounds include paper and pulp mill byproducts, pharmaceuticals, diverse types of plastics, hydrocarbons, pigments, and dyes, as well as pesticides and insecticides. Fungal bioremediation stands out as a promising technology that uses the metabolic potential of fungi to eliminate or mitigate the impact of pollutants. Notably, species of the genus Pycnoporus exhibit significant capabilities for degrading a broad spectrum of toxic molecules. This degradation is facilitated by released ligninolytic enzymes, especially laccase, and cellular enzymes pertaining to the cytochrome P450 monooxygenase system. The laccase, which is overproduced by the genus Pycnoporus, is quite remarkable for its high redox potential. The objective of this review is to highlight the proficiency of the Pycnoporus genus in the degradation of pollutants in submerged and solid-state fermentation. Recent studies conducted over the past decade consistently highlight the Pycnoporus genus as a robust contender in the realm of white biotechnology.

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Section: Decolorization and Degradation Of Synthetic Dyes By Pycnopor...mentioning

confidence: 99%

Biotransformation of Pollutants by Pycnoporus spp. in Submerged and Solid-State Fermentation: Mechanisms, Achievements, and Perspectives

Cheute,

Uber,

dos Santos

et al. 2024

Biomass

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“…Additionally, a common substrate for laccase is phenol, a toxic pollutant which is dangerous to humans 6 . The use of laccases as versatile agents of bioremediation is emerging as a sustainable option to degrade chemical pollutants, and there is interest in investigating the catalytic mechanisms of laccase to optimize its function in bioremediation 8 .…”

Section: Introductionmentioning

confidence: 99%

A food color based colorimetric assay forCryptococcus neoformanslaccase activity

Ramirez,

Dragotakes,

Casadevall

2024

Preprint

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Cryptococcus neoformans is a fungal pathogen that causes cryptococcosis mostly in immune compromised patients, such as those with HIV/AIDS. One survival mechanism of C. neoformans during infection is melanin production, which is catalyzed by laccase. Hence comparative assessment of laccase activity is useful for characterizing cryptococcal strains. After a serendipitous observation that culturing C. neoformans with food coloring resulted in the degradation of some dyes with phenolic structures, we associated this phenomenon with catalytic activity by cryptococcal laccase. Consequently, we investigated the color changes for the food dyes metabolized by C. neoformans laccase and explored using this effect for the development of colorimetric assays to measure laccase activity. We developed several versions of a food dye based colorimetric laccase assay that can be used to compare the relative laccase activities between different C. neoformans strains. We found that phenolic color degradation was glucose dependent, which may reflect changes in the reduction properties of the media. Our food color based colorimetric assay has several advantages over the commonly used 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay for laccase activity, including lower cost, no reversibility and easier application since it does not require constant monitoring. Our method has potential applications in environmental fields, since laccases can be used to degrade pollutants in bodies of water, and this is an efficient test to determine laccase activity. Additionally, it is useful for comparing laccase activity across different C. neoformans strains, which can provide insight into the expression of this virulence factor across strains.

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“…It can oxidize synthetic dyes, azo dyes, phenolic compounds, herbicides, chlorinated phenols, dioxins, pesticides, cresols, and several pharmaceutical products [6]. Laccases are also used in the industry category as bioleaching for pulp, textile, and paper industries, biotransformation, and fruit juice clarification [7,8]. Fungal laccases have a higher redox potential (E•) than bacterial and plant laccases.…”

Section: Introductionmentioning

confidence: 99%

“…Different chemical and physical strategies have been misused for dye and other contaminants elimination, like adsorption, constructed wetlands, cavitation, membrane filtration, ion exchange, ultrasound technique, oxidation, photocatalytic ozonation, precipitation, coagulation, flocculation, and electrochemical strategies [8,31,32]. Despite all these previous methods for dye removal, the removal process has a high cost and a high probability of secondary contamination; some require high energy and non-specificity, could be highly toxic, and some generate sludge problems [25,33].…”

Section: Introductionmentioning

confidence: 99%

Kinetic studies on optimized extracellular laccase from Trichoderma harzianum PP389612 and its capabilities for azo dye removal

Abd El-latif,

Zohri,

El-Aref

et al. 2024

Microb Cell Fact

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Background Azo dyes represent a common textile dye preferred for its high stability on fabrics in various harsh conditions. Although these dyes pose high-risk levels for all biological forms, fungal laccase is known as a green catalyst for its ability to oxidize numerous dyes. Methods Trichoderma isolates were identified and tested for laccase production. Laccase production was optimized using Plackett–Burman Design. Laccase molecular weight and the kinetic properties of the enzyme, including Km and Vmax, pH, temperature, and ionic strength, were detected. Azo dye removal efficiency by laccase enzyme was detected for Congo red, methylene blue, and methyl orange. Results Eight out of nine Trichoderma isolates were laccase producers. Laccase production efficiency was optimized by the superior strain T. harzianum PP389612, increasing production from 1.6 to 2.89 U/ml. In SDS-PAGE, purified laccases appear as a single protein band with a molecular weight of 41.00 kDa. Km and Vmax values were 146.12 μmol guaiacol and 3.82 μmol guaiacol/min. Its activity was stable in the pH range of 5–7, with an optimum temperature range of 40 to 50 °C, optimum ionic strength of 50 mM NaCl, and thermostability properties up to 90 °C. The decolorization efficiency of laccase was increased by increasing the time and reached its maximum after 72 h. The highest efficiency was achieved in Congo red decolorization, which reached 99% after 72 h, followed by methylene blue at 72%, while methyl orange decolorization efficiency was 68.5%. Conclusion Trichoderma laccase can be used as an effective natural bio-agent for dye removal because it is stable and removes colors very well.

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A review on the laccase assisted decolourization of dyes: Recent trends and research progress

Cited by 29 publications

References 115 publications

Biotransformation of Pollutants by Pycnoporus spp. in Submerged and Solid-State Fermentation: Mechanisms, Achievements, and Perspectives

Biotransformation of Pollutants by Pycnoporus spp. in Submerged and Solid-State Fermentation: Mechanisms, Achievements, and Perspectives

A food color based colorimetric assay forCryptococcus neoformanslaccase activity

Kinetic studies on optimized extracellular laccase from Trichoderma harzianum PP389612 and its capabilities for azo dye removal

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