A review on the measurement of the bending rigidity of lipid membranes

Karal, Mohammad Abu Sayem; Billah, Md. Masum; Ahmed, Marzuk; Ahamed, Md. Kabir

doi:10.1039/d3sm00882g

Cited by 8 publications

(3 citation statements)

References 171 publications

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“…In this mechanism, the membrane fusion is thought to occur through intermediate structure known as ‘stalk’. This stalk is form due to the bending energy (related to bending modulus) of the lipid bilayer [ 57 ]. Theoretical analysis of the stalk has allowed to determine the elastic energy associated with this structure and make predictions regarding the possible evolution of the overall process by assuming that the stalk has a shape of revolution of a circular arc.…”

Section: Discussionmentioning

confidence: 99%

Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses

Bhuiyan,

Karal,

Orchi

et al. 2024

PLoS ONE

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Irreversible electroporation induces permanent permeabilization of lipid membranes of vesicles, resulting in vesicle rupture upon the application of a pulsed electric field. Electrofusion is a phenomenon wherein neighboring vesicles can be induced to fuse by exposing them to a pulsed electric field. We focus how the frequency of direct current (DC) pulses of electric field impacts rupture and electrofusion in cell-sized giant unilamellar vesicles (GUVs) prepared in a physiological buffer. The average time, probability, and kinetics of rupture and electrofusion in GUVs have been explored at frequency 500, 800, 1050, and 1250 Hz. The average time of rupture of many ‘single GUVs’ decreases with the increase in frequency, whereas electrofusion shows the opposite trend. At 500 Hz, the rupture probability stands at 0.45 ± 0.02, while the electrofusion probability is 0.71 ± 0.01. However, at 1250 Hz, the rupture probability increases to 0.69 ± 0.03, whereas the electrofusion probability decreases to 0.46 ± 0.03. Furthermore, when considering kinetics, at 500 Hz, the rate constant of rupture is (0.8 ± 0.1)×10−2 s-1, and the rate constant of fusion is (2.4 ± 0.1)×10−2 s-1. In contrast, at 1250 Hz, the rate constant of rupture is (2.3 ± 0.8)×10−2 s-1, and the rate constant of electrofusion is (1.0 ± 0.1)×10−2 s-1. These results are discussed by considering the electrical model of the lipid bilayer and the energy barrier of a prepore.

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Section: Discussionmentioning

confidence: 99%

Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses

Bhuiyan,

Karal,

Orchi

et al. 2024

PLoS ONE

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“…As a material consisting of polymer mixtures, empty LNPs exhibit polymer-like temperature-induced 6/18 phase transition behaviors, a typical characteristic of which is enhanced fluidity of lipids 42 . Differential scanning calorimetry experiment suggests that our empty LNPs has a critical phase transition temperature (𝑇 𝑝 ) range roughly from 39 ℃ to 53 ℃ (Supplementary Fig.…”

Section: Impact Of Temperaturementioning

confidence: 99%

Capsule coffee-inspired customizable production of nucleic acid medicine using a rapid on-site microfluidic assembly (ROMA) technology

Xu,

Zheng,

Wang

et al. 2024

Preprint

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The emergence of nucleic acid medicine (NAM) has profoundly addressed global infectious disease challenges1 and revolutionized cancer therapy2 and gene-editing3. However, current NAM, such as mRNA vaccine, remains distant from customizable supply and is fraught with hurdles of thermo-instability and infrastructure dependence. Drawing inspiration from the efficiency of capsule coffee machines, we introduce a Rapid On-site Microfluidic Assembly (ROMA) prototype capable of generating ready-to-inject mRNA vaccines at a throughput of 180 doses/hour (∼100 µg mRNA/dose). Analogous to the varied flavors of capsule coffee, our ROMA prototype offers personalized options for mRNA vaccines, including lipid nanoparticle (LNP) sizes, compositions, mRNA types, and dosages tailored to individual needs. Diverging from traditional mechanism of directly assembling mRNA and lipids into mRNA-LNPs, ROMA technology utilizes mRNA and pre-made empty LNPs to form mRNA-LNPs. Nevertheless, ROMA mRNA vaccine exhibits equivalent physiochemical parameters and in-vivo expressions compared to conventional ones, with a benefit of lower toxicity. Crucially, ROMA mRNA vaccine, immediately deployable without the need for storage, fundamentally avoids the thermal instability and degradation risks associated with conventional mRNA vaccines. This transformative ROMA technology offers unparalleled user-end convenience, unlocking the potential for personalized mRNA vaccines and treatments, thereby significantly expanding the scope of NAM therapeutics.

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“…Tether force includes contributions not only from plasma membrane tension but also from membranecytoskeleton interactions and bilayer bending rigidity, 37,[59][60][61][62][63] which is compositiondependent. 64 Therefore it remains unclear to what extent the reported changes in tether force stem from actual membrane tension gradients, from changes in membrane-cortex binding energy, or from lipid composition changes. Moreover, limited by discrete spatial measurements of the dorsal cellular plasma membrane, tube pulling cannot provide insights into the shape of the gradients nor investigate the tension distributions of the ventral cell membrane in contact with the substrate.…”

Section: Introductionmentioning

confidence: 99%

Actin dynamics sustains spatial gradients of membrane tension in adherent cells

García-Arcos,

Mehidi,

Velázquez

et al. 2024

Preprint

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Tension propagates in lipid bilayers over hundreds of microns within milliseconds, precluding the formation of tension gradients. Nevertheless, plasma membrane tension gradients have been evidenced in migrating cells and along axons. Here, using a fluorescent membrane tension probe, we show that membrane tension gradients exist in all adherent cells, whether they migrate or not. Non-adhering cells do not display tension gradients. We further show that branched actin increases tension, while membrane-to-cortex attachments facilitate its propagation. Tension is the lowest at the edge of adhesion sites and highest at protrusions, setting the boundaries of the tension gradients. By providing a quantitative and mechanistic basis behind the organization of membrane tension gradients, our work explains how they are actively sustained in adherent cells.

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A review on the measurement of the bending rigidity of lipid membranes

Abstract: This review outlines the techniques used to assess the membrane bending rigidity and presents the findings obtained by manipulating membrane constituents and experimental conditions. This could have implications for biomedical research applications.

Cited by 8 publications

References 171 publications

Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses

Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses

Capsule coffee-inspired customizable production of nucleic acid medicine using a rapid on-site microfluidic assembly (ROMA) technology

Actin dynamics sustains spatial gradients of membrane tension in adherent cells

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