A review on the pharmacological properties, toxicological characteristics, pathogenic mechanism and analytical methods of aristolochic acids

Zhang, Miaomiao; Liu, Haiyan; Han, Yamei; Bai, Ligai; Yan, Hongyuan

doi:10.1080/15569543.2020.1811344

Cited by 3 publications

(2 citation statements)

References 78 publications

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“…Besides names and structures, inconsistency was also found regarding the sources of DNA adducts (see Supplementary Table S4). In this database, the source was associated to DNA adducts based on literature reviews (Bakhiya and Appel, 2010;Turesky and Le Marchand, 2011;Wei and Yin, 2015;Lee and Ryu, 2016;Stegelmeier et al, 2016;Yu et al, 2016;Alshannaq and Yu, 2017;Ma et al, 2019;Zhang et al, 2021;Eisenreich et al, 2021), or based on the specific publication where the adduct was identified. Both the causative genotoxin and its source have been reported (The DNA adduct database in Word format).…”

Section: Data Curation and Harmonizationmentioning

confidence: 99%

A Comprehensive Database for DNA Adductomics

et al. 2022

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The exposure of human DNA to genotoxic compounds induces the formation of covalent DNA adducts, which may contribute to the initiation of carcinogenesis. Liquid chromatography (LC) coupled with high-resolution mass spectrometry (HRMS) is a powerful tool for DNA adductomics, a new research field aiming at screening known and unknown DNA adducts in biological samples. The lack of databases and bioinformatics tool in this field limits the applicability of DNA adductomics. Establishing a comprehensive database will make the identification process faster and more efficient and will provide new insight into the occurrence of DNA modification from a wide range of genotoxicants. In this paper, we present a four-step approach used to compile and curate a database for the annotation of DNA adducts in biological samples. The first step included a literature search, selecting only DNA adducts that were unequivocally identified by either comparison with reference standards or with nuclear magnetic resonance (NMR), and tentatively identified by tandem HRMS/MS. The second step consisted in harmonizing structures, molecular formulas, and names, for building a systematic database of 279 DNA adducts. The source, the study design and the technique used for DNA adduct identification were reported. The third step consisted in implementing the database with 303 new potential DNA adducts coming from different combinations of genotoxicants with nucleobases, and reporting monoisotopic masses, chemical formulas, .cdxml files, .mol files, SMILES, InChI, InChIKey and IUPAC nomenclature. In the fourth step, a preliminary spectral library was built by acquiring experimental MS/MS spectra of 15 reference standards, generating in silico MS/MS fragments for all the adducts, and reporting both experimental and predicted fragments into interactive web datatables. The database, including 582 entries, is publicly available (https://gitlab.com/nexs-metabolomics/projects/dna_adductomics_database). This database is a powerful tool for the annotation of DNA adducts measured in (HR)MS. The inclusion of metadata indicating the source of DNA adducts, the study design and technique used, allows for prioritization of the DNA adducts of interests and/or to enhance the annotation confidence. DNA adducts identification can be further improved by integrating the present database with the generation of authentic MS/MS spectra, and with user-friendly bioinformatics tools.

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Section: Data Curation and Harmonizationmentioning

confidence: 99%

A Comprehensive Database for DNA Adductomics

et al. 2022

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“…It is extensively studied for various pharmacological activities such as antibacterial, anti-fungal 3 , anti-diabetic and anti-inflammatory 4 activity. The compound aristolochic acid present in Apama siliquosa is expected to show tumor inhibitory activity hence could be used for cancer therapy 5 .…”

Section: Introductionmentioning

confidence: 99%

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2021

IJPSR

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The demand for herbal medicines is growing at a drastic rate due to their improved pharmacological actions, minimal side effects, and costeffectiveness. The ever-increasing cost of chemotherapy ruined the economic stability of many families. This led to the discovery of herbal medicines for cancer treatment. Tribes of Western Ghats have been using the roots of Apama siliquosa for cytotoxic activity. The methanolic and aqueous root extracts of Apama siliquosa Lamk were used to prepare different concentrations. These different dilutions of extract were used to carry out brine shrimp lethality assay & the LC 50 value was determined. Cell line study by MTT assay using cancerous cell line (MCF7 cells-human breast adenocarcinoma cells) & LC 50 value was determined. GCMS study on plant extract was carried out. The methanolic extract shows the presence of Caryophyllene oxide (m/z 220) methyl ester of Aristolochic acid I (m/z 355), Aristolactam I (m/z 295). Aristo lactam II (m/z 265), and Aristo lactam III (m/z 281). The aqueous extract shows the presence of Aristo lactam I (m/z 280). Aristo lactam II (m/z 264). Caryophyllene oxide and Aristolochic acids are terpene and alkaloid, respectively. LC 50 value for aqueous and methanolic extract was found to be 102.32µg/ml & 91.20µg/ml respectively by brine shrimp lethality assay and the same by MTT assay was found to be 117.527µg/ml & 87.4056µg/ml respectively for breast cancer cell line (MCF7 cells). These led to the conclusion that both extracts showed excellent toxicity to the naupli and mild toxicity to cell lines.

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