A revised description of Haplosporidium armoricanum , parasite of Ostrea edulis L. from Galicia, northwestern Spain, with special reference to the spore-wall filaments

Azevedo, Carlos; Montes, Jaime; Corral, Laura

doi:10.1007/s004360050669

Cited by 21 publications

(26 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lack of a precise and detailed deWnition of the terms used to describe these structures has caused some taxonomic problems (Azevedo, 2001;Burreson, 2001;Flores et al, 1996;McGovern and Burreson, 1990). Beside some controversial opinions (see Burreson, 2001;Perkins, 1991Perkins, , 2000, the genus Haplosporidium has been deWned as species whose spores possess tails or Wlaments attached to the spore wall, which are formed by the same material as the wall (Azevedo et al, 1999(Azevedo et al, , 2003Burreson, 2001;Ciancio et al, 1999;Hine and Thorne, 1998;Ormières, 1980;Sprague, 1979;Taylor, 1986). On the other hand, Minchinia spp.…”

Section: Introductionmentioning

confidence: 96%

Ultrastructural and molecular characterization of Haplosporidium montforti n. sp., parasite of the European abalone Haliotis tuberculata

Azevedo

Balseiro

Casal

et al. 2006

Journal of Invertebrate Pathology

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 96%

Ultrastructural and molecular characterization of Haplosporidium montforti n. sp., parasite of the European abalone Haliotis tuberculata

Azevedo

Balseiro

Casal

et al. 2006

Journal of Invertebrate Pathology

Self Cite

View full text Add to dashboard Cite

“…Haplosporidium armoricanum from Ostrea edulis in Europe has paired filaments arising from each end of the spore (Azevedo et al 1999). Haplosporidium nelsoni and H. costale from Crassostrea virginica along the east coast of the United States do not have paired filaments arising from the posterior end of the spore; rather they have wrappings around the spore (Burreson and Reece, 2006).…”

Section: Discussionmentioning

confidence: 99%

Spore ornamentation of Haplosporidium hinei n. sp. (Haplosporidia) in pearl oysters Pinctada maxima (Jameson, 1901)

et al. 2008

View full text Add to dashboard Cite

An infection of pearl oysters, Pinctada maxima, attributed to a Haplosporidium sp. by Hine and Thorne (1998) has been detected on 3 occasions and is considered to represent a serious concern to the pearling industry in Australia. The spore ornamentation of the parasite was determined by scanning electron microscopy and transmission electron microscopy. Spores of the parasite were pleomorphic, or elongated 3 . 5-4 mmr2 . 5-3 . 0 mm in size. Two filaments were wound around the spore and originated from 2 ' knob-like ' posterior thickenings. Both filaments passed up one side of the spore together until just below the operculum whereupon each split and passed obliquely under the lip of the opercula lid. Each filament wrapped around the spore 4 times. The posterior thickenings seem to appear late in the development of the spore and were composed of spore wall material. A second set of branching tubular filaments composed of a different material was observed on the spore body although not on mature spores possessing a ' knob-like ' posterior thickening. The ornamentation on the spores of the pearl oyster parasite was unique amongst described haplosporidian species where spore ornamentation is known. The parasite is named in this manuscript as Haplosporidium hinei n. sp.

show abstract

“…In Galicia, NW Spain, haplosporidian parasites have been described in clams Ruditapes decussatus (Figueras et al 1992), in cockles Cerastoderma edule (Carballal et al 2001) and also in flat oysters Ostrea edulis L. (Azevedo et al 1999). In the latter species, by a study of the spore morphology, Azevedo et al (1999) named the parasite Haplosporidium armoricarum.…”

Section: Discussionmentioning

confidence: 99%

“…In the latter species, by a study of the spore morphology, Azevedo et al (1999) named the parasite Haplosporidium armoricarum. In Portugal, haplosporidians found in clams were identified as Minchinia tapetis by Azevedo (2001) after ultrastructural characterization of spores allowed him to assign this parasite to the Minchinia genus.…”

Section: Discussionmentioning

confidence: 99%

“…Haplosporidian parasites, mainly Haplosporidium nelsoni and H. costale, have been associated with extensive oyster mortalities in the United States (Andrews 1966, Ford & Haskin 1982. Haplosporidiosis has also been detected in Europe in flat oysters Ostrea edulis (Van Banning 1977, Azevedo et al 1999, in Pacific oysters Crassostrea gigas (Renault et al 2000), in cockles Cerastoderma edule (Carballal et al 2001) and in clams Ruditapes decussatus L (Azevedo 2001). Perkins (1990Perkins ( , 1991 included 3 genera in the phylum Haplosporidia: Minchinia, Haplosporidium and Urosporidium.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular detection of a haplosporidian parasite in carpet shell clam Ruditapes decussatus from Spain

Novoa

Balseiro²,

Figueras

2004

Dis. Aquat. Org.

View full text Add to dashboard Cite

A protozoan parasite identified by histology as a haplosporidian was detected in carpet shell clam Ruditapes decussatus from Spain during routine samplings. Analysis based on the sequence of the small subunit ribosomal RNA gene revealed that this organism was related to the haplosporidian group, mainly to Urosporidium. A specific probe was used for in situ hybridization studies to show the specificity of the amplified sequence. KEY WORDS: Haplosporidian · Clam · Ruditapes decussatus Resale or republication not permitted without written consent of the publisherDis Aquat Org 61: [89][90][91][92][93] 2004 2002. The animals (n = 184) were processed immediately after being received at the laboratory.Histology. Soft tissues were fixed in Davidson's fixative (Shaw & Battle 1957) for 24 h, and a transverse section approximately 5 mm thick including mantle, gonad, digestive gland, gills, kidney, and foot was excised from each clam. Tissue samples were embedded in paraffin, and 5 µm sections were stained with hematoxylin-eosin. The histological sections were observed under a light microscope (Nikon).Amplification of the 18S rRNA gene and sequencing. DNA from carpet shell clams was extracted using DNAzol (Invitrogen) following the recommended protocol.The 18S rRNA gene was amplified by polymerase chain reaction (PCR) using several pairs of conserved primers derived from known conserved regions of the 18S rRNA gene (Medlin et al. 1988, Novoa et al. 2002 (U1S: 5'-AACCTGGTT-GATCCTGCCAGT-3'; 336AS: 5'-GCTCCCTCTCC-GGAATCGAA-3'; Cas2AS: 5'-ACGGGCGGTGTTC-AAAGG-3'; U3S: 5'-GCTACCACATCCAAGGAA-3'; U4AS: 5'-GTCTCGTTCGTTAACGG-3'). PCR was performed in a total volume of 25 µl containing 1 µl 10 mM dNTP mix, 0.125 µl Taq polymerase (Roche), 2.5 µl Taq 10× buffer, 1.25 µl 25 mM MgCl 2 , 1.25 µl of each primer (100 µM) and 1 µl of DNA. Following an initial denaturation at 94°C, reactions were subjected to 40 cycles of initial denaturation at 94°C for 1 min, annealing at 50°C for 1 min and extension at 72°C for 2 min. A final extension of 10 min at 72°C was also carried out. PCR products were visualized on a 1% agarose gel stained with ethidium bromide. A 100 bp ladder standard (Amersham Pharmacia) was also included on the gel.PCR products were cloned directly in the vector pCR 2.1 (Invitrogen) following the standard protocol supplied by the manufacturers (TA Cloning Kit, Invitrogen) and then used to transform competent Escherichia coli TOP10 F'. Screening of clones carrying 18S rRNA-coding region fragments was performed by PCR adding the positive colony directly to the PCR mixture reaction using the vector's M13 forward primer. Positive clones were sequenced as described below in order to determine if the gene fragment corresponded to haplosporidians or to other organisms, mainly clams. PCR products were purified by digestion with the enzymes exonuclease I and shrimp phosphatase (SAP) (Amersham Pharmacia Biotech) for 1 h at 37°C. The enzymes were then denatured for 15 min at 80°C. The sequencing reactions of purified PCR prod...

show abstract

A revised description of Haplosporidium armoricanum , parasite of Ostrea edulis L. from Galicia, northwestern Spain, with special reference to the spore-wall filaments

Cited by 21 publications

References 20 publications

Ultrastructural and molecular characterization of Haplosporidium montforti n. sp., parasite of the European abalone Haliotis tuberculata

Ultrastructural and molecular characterization of Haplosporidium montforti n. sp., parasite of the European abalone Haliotis tuberculata

Spore ornamentation of Haplosporidium hinei n. sp. (Haplosporidia) in pearl oysters Pinctada maxima (Jameson, 1901)

Molecular detection of a haplosporidian parasite in carpet shell clam Ruditapes decussatus from Spain

Contact Info

Product

Resources

About