A revised method for the application of API 50 CH carbohydrate kits to yeasts

Ison, R. W.

doi:10.1111/j.1472-765x.1987.tb01570.x

Cited by 9 publications

(6 citation statements)

References 5 publications

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“…Utilization of various carbon sources was determined and performed using API 50 CH indicator strips and 50 CHB medium purchased from BioMerieux following the instruction manual (see Supporting Information). [ 43 ]…”

Section: Methodsmentioning

confidence: 99%

Next‐Generation Microbial Workhorses: Comparative Genomic Analysis of Fast‐Growing Vibrio Strains Reveals Their Biotechnological Potential

Yuan

Xiao

et al. 2020

Biotechnology Journal

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Vibrio is a recognized fast-growing bacterial genus, which is considered to be attractive for the development of next-generation biotechnological workhorses. Here, three Vibrio strains FA1, FA2, and FA3, capable of growing rapidly in cost-effective media, are isolated and systematically evaluated. Genome sequencing and comparative genomic analyses are performed to reveal the underlying genetic differences between the strains and estimate their biotechnological potential. Studies of their phylogenetic tree, colinear visualization, and orthology uncover some difference in the gene content related to cell growth of the four Vibrio strains FA1, FA2, FA3, and ATCC 14048, which may explain growth superiority of the isolated strains. It is noted that there are more copies of several genes related to the DNA replication in the FA2 genome than in the other compared Vibrio strains. Furthermore, the genes responsible for amino synthesis are found, such as asD, within strains FA1 and FA2. Gene cluster cadABC, which relates to cell adaptation at acidic pH, only exists in strains FA1, FA2, and FA3. Finally, the wide spectra of substrates and genetic operability of these three isolated Vibrio strains are initially verified. This study provides excellent candidates for the development of next-generation fast-growing microbial workhorses, which may be very useful in synthetic biology.

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Section: Methodsmentioning

confidence: 99%

Next‐Generation Microbial Workhorses: Comparative Genomic Analysis of Fast‐Growing Vibrio Strains Reveals Their Biotechnological Potential

Yuan

Xiao

et al. 2020

Biotechnology Journal

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“…Divers systèmes d'identification rapide sont aujourd'hui commercialisés sous forme de kit. Malheureusement, la mise en oeuvre d'un seul système ne conduit pas toujours à l'identification des levures autres que celle d'intérêt médical (Ison, 1987 ;Deak et Beuchat, 1988 ;Heard et Fleet, 1990 ;Rohm et al, 1990 ;Seiler, 1991 ;Deak, 1993 (Barnett et al,199Ob). La nomenclature utilisée ici est par consé-quent celle de Barnett et al (1990a Soumises aux tests API 32 C et API ANAER amendés, les 2 souches ne diffèrent qu'au niveau de l'esculine que seule la souche blanche assimile.…”

Section: Levuresunclassified

Inventaire et évolution des flores fongiques de surface du reblochon de Savoie

Bärtschi

Berthier

Valla

1994

Lait

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“…The API system is intended to identify a wide range of species but the tests covered are too few to permit unequivocal identification (Subden et al 1980;Fuchs & Dolan 1982). The API 50 CH galleries, which include 49 carbon assimilation or fermentation tests, have been used for the general identification of yeasts, but further studies with this system are required (Schmidt 1980;Ison 1987). The API 50 CH system was specifically developed for the identification of bacteria and, in our experience, it appears that the concentrations ( 0 .…”

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confidence: 99%

A convenient microtitre tray procedure for yeast identification

Heard

Fleet

1990

Journal of Applied Bacteriology

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Media for yeast identification tests were incorporated into the wells of a microtitre tray. The tests included fermentation and assimilation of carbohydrates, assimilation of nitrogen compounds, growth in vitamin-free medium, resistance to cycloheximide, and observations for cell morphology and sporulation. Results of tests conducted in the trays showed very good agreement with those obtained by conventional methods. Eighteen reference yeasts were correctly identified from tests conducted in the trays. The trays of media could be stored, and provided a convenient system for yeast identification.

show abstract

A revised method for the application of API 50 CH carbohydrate kits to yeasts

Cited by 9 publications

References 5 publications

Next‐Generation Microbial Workhorses: Comparative Genomic Analysis of Fast‐Growing Vibrio Strains Reveals Their Biotechnological Potential

Next‐Generation Microbial Workhorses: Comparative Genomic Analysis of Fast‐Growing Vibrio Strains Reveals Their Biotechnological Potential

Inventaire et évolution des flores fongiques de surface du reblochon de Savoie

A convenient microtitre tray procedure for yeast identification

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