A revision of current data and views on membrane hydrolysis and transport in the mammalian small intestine based on a comparison of techniques of chronic and acute experiments: Experimental re-investigation and critical review

Ugolev, A. M.; Zaripov, B Z; Iezuitova, N N; Груздков, А. А.; Rybin, I S; Voloshenovich, M I; Никитина, А. А.; MYu, Punin; Tokgaev, N T

doi:10.1016/0300-9629(86)90269-0

Cited by 25 publications

(24 citation statements)

References 37 publications

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“…It means that surgery leads to increasing of the muscle tissue (possibly because of the partial replacing with the connective tissue as a result of operation). It comports to the data obtained by Ugolev et al [20,21]. …”

Section: Discussionsupporting

confidence: 71%

“…It's also essential to compare the difference in the velocity of perfusion in our experiments and on the isolated loop [4]. In our experiments we always use 0.6 ml/min as the most physiological condition [2,9]. Gromova and Gruzdkov used 0.25-0.3 ml/min especially for experiments with glycine because of the relatively low rate of the free glycine absorption compare to glucose [19].…”

Section: Discussionmentioning

confidence: 99%

“…However the main problem of this method is an isolation of the investigated intestine fragment from the alimentary canal. As the result it does not get the endogenic factors and exogenic substrates and experience fast atrophy with the decreasing of the digestive and absorptive ability [4,8,9]. It triggered the search for a new ways and under the leadership of academic Ugolev A.M. we created the method of formation and investigation of a functioning fragment of a rat's small intestine in the chronic experiments under physiological condition in vivo [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition

Ov¹

2014

J Gastrointest Dig Syst

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Objectives and method: To determine the velocity of glycine absorption in the chronic experiments under physiological condition with no operation trauma, pain, narcosis and atrophy of the small intestine using and original method of surgical formation of the functioning fragment of the small intestine with 'living fistulas' in the presence of chime, all gastrointestinal secrets and natural innervations. Results:The glycine absorption velocity increases during one hour of perfusion. Absolute parameters of absorptive activity of the small intestine in the chronic experiments in vivo are higher than in the isolated loop of the rats' small intestine. We observed no dissolution of perfusate with gastrointestinal fluids in the small intestine functioning part indicating that absorption of water in this fragment of small intestine prevails. Conclusions:Formation and perfusion of the functioning fragment of the rats' small intestine is an adequate approach to the investigating the activity of the small intestine under physiological condition. It allows detecting the impact of the regulatory activity of chime (its exogenic and endogenic components).

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition

Ov¹

2014

J Gastrointest Dig Syst

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“…The experiments were conducted on five adult Wistar rats (male, weight 180 -300 g) with an isolated ThiryVella segment of the proximal jejunum (L ~ 20 cm) as described previously [14][15][16]. The surgery technique, preparing of the animals for the experiments, as well as the experimental conditions were similar to the original method with slight modifications.…”

Section: Chronic Experimentsmentioning

confidence: 99%

“…First, the assumption is based on the results of in vitro or in vivo experiments on anesthetized animals. However, it has been shown previously [14][15][16], that acute experiments in vivo are far from physiological conditions because of the effect of anesthesia and surgical trauma.…”

Section: Introductionmentioning

confidence: 99%

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

Груздков¹,

Громова²,

Grefner³

et al. 2012

JBPC

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Previous studies have shown two components of glucose absorption in the small intestine: a secondary active transport through SGLT1, and unsaturated component, recently attributed mainly to the facilitated diffusion through GLUT2, but the relationship between these two components under physiological conditions remains controversial. In chronic experiments on nonanesthetized rats we investigated for the first time the kinetics of maltose hydrolysis and glucose absorption in the isolated loop of the small intestine in a wide range of maltose and glucose concentrations (25 -200 mmol/l glucose). The processes were simulated on mathematical models which took into account the current views about mechanisms of hydrolysis and transport of nutrients and geometric characteristics of the intestinal surface. The results of chronic experiments and mathematical simulation have shown that under the close to physiological conditions the glucose transport mediated by SGLT1 is the main mechanism of its absorption in comparison with the unsaturated component. This was demonstrated not only at low, but also at high substrate concentrations. We conclude that correct evaluation of the relative contribution of different mechanisms in glucose transport through the intestinal epithelium requires taking into account the geometric specificities of its surface.

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Low intake of digestible carbohydrates ameliorates duodenal absorption of carbohydrates in mice with glucose metabolism disorders induced by artificial sweeteners

et al. 2019

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Background Long‐term artificial sweetener consumption has been reported to induce glucose intolerance, and the intestinal microbiota seems as an important target. While the impacts of artificial sweeteners on energy balance remain controversial, this work aimed to evaluate the protective effects in mice of a low digestible carbohydrate (LDC) diet on plasma glucose, plasma fasting insulin, sweet taste receptors, glucose transporters, and absorption of carbohydrates, together with consumption of acesulfame potassium (AK) or saccharin (SAC). Results Artificial sweetener was administered to mice for 12 weeks to induce glucose metabolism disorders; mice were treated with an LDC diet for the final 6 weeks. The experimental groups were treated with an LDC diet that had the same energy as the normal‐diet group. Prolonged administration of artificial sweeteners led to metabolic dysfunction, characterized by significantly increased plasma glucose, insulin resistance, sweet taste receptors, glucose transporters, and absorption of carbohydrates. Treatment with an LDC diet positively modulated these altered parameters, suggesting overall beneficial effects of an LDC diet on detrimental changes associated with artificial sweeteners. Conclusions Reducing digestible carbohydrates in the diet can significantly reduce the absorption of carbohydrates and improve glucose metabolism disorders caused by dietary factors. These effects may be due to the fact that reducing the amount of digestible carbohydrates in the feed can reduce the number of intestinal sweet receptors induced by exposure to artificial sweeteners. © 2019 Society of Chemical Industry

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A revision of current data and views on membrane hydrolysis and transport in the mammalian small intestine based on a comparison of techniques of chronic and acute experiments: Experimental re-investigation and critical review

Cited by 25 publications

References 37 publications

Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition

Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

Low intake of digestible carbohydrates ameliorates duodenal absorption of carbohydrates in mice with glucose metabolism disorders induced by artificial sweeteners

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