Spines are a striking feature of cacti and display wide variation in size, number, shape, and texture. This study showed that Turbinicarpus species exhibit not only a high variability in the gross morphology of the spines, but also in their micro-morphology. Their surface can be smooth or ornamented with projections that can be low, conical, pinnate, or long trichomes. The epidermis can be continuous, broken up into single cell elements or transversely fi ssured, the fi ssures extending deeply into the underlying sclerenchyma. The mechanical properties of the spines are related to their anatomy, here documented for the fi rst time. The woody rigid spines being made up of fi bers with thick walls (> 3 μm), while papery or corky spines have a sclerenchyma made up of fi bers with thin walls (< 2 μm). Alternatively, spine anatomy can be dimorphic with the outermost layers made up of thin-walled fi bers and an inner core made up of thick-walled fi bers. Turbinicarpus taxonomy mostly relies on spine features and the newly collected data can contribute to a better understanding of the interspecifi c relationships. The epidermal features like long trichomes or the lack of ornamentation as well as the modifi ed anatomy of corky spines may be apomorphic characters within the genus.