A ribozyme with michaelase activity

Eisenführ, Alexander; Arora, Paramjit S.; Sengle, Gerhard; Takaoka, Leo R; Nowick, James S.; Famulok, Michael

doi:10.1016/s0968-0896(02)00311-5

Cited by 57 publications

(44 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The crude residue was then purified by column chromatography (DCM-MeOH, 100% to 95 : 5 using 0.5% increments; R f = 0.44) to give a colourless oil, existing as a pair of rotamers (417 mg, 79%). 1 Diethyl-3-(4-azido-8,9-dioxo-2,6-diazabicyclo[5.2.0]non-1(7)-en-2-yl)propyl)phosphonate, 16.…”

Section: Methodsmentioning

confidence: 99%

Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: synthesis and evaluation in cellulo

et al. 2014

View full text Add to dashboard Cite

A second generation of N-methyl-d-aspartate (NMDA) receptor-targeted MRI contrast agents has been synthesised and evaluated in cellulo, based on established bicyclic NMDA receptor antagonists. Their use as responsive MR imaging probes has been evaluated in suspensions of NSC-34 cells, and one agent exhibited significant enhancements in measured longitudinal and transverse water proton relaxation rates (19 and 38% respectively; 3 T, 298 K). A biotin derivative of the lead compound was prepared and the specificity and reversibility of binding to the NMDA cell surface receptors demonstrated using confocal laser scanning microscopy. Competitive and reversible binding of glutamate to the receptors was also visualised, suggesting that the receptor-targeted approach may allow MRI to be used to monitor neuronal events associated with modulation of local glutamate concentrations.

show abstract

Section: Methodsmentioning

confidence: 99%

Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: synthesis and evaluation in cellulo

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Durch eine In-vitro-Transkription mit T7-RNA-Polymerase unter Verwendung so genannter Initiatornucleotide wie Guanosinmonophosphothioat (GMPS) können alternativ Modifikationen am 5'-Ende von RNA-Molekülen enzymatisch eingeführt werden. [69,73,74] Der Einbau von GMPS am 5'-Ende der RNA ermöglicht die Modifizierung von RNA-Molekülen mit fluoreszierenden Resten oder anderen Reportermolekülen mithilfe des Iodacetamidoverfahrens (Schema 4). [69] Unlängst entwickelten Jäschke et al ein Initiatornucleotid, mit dem Aldehydmodifikationen am 5'-Ende der enzymatisch synthetisierten RNA eingeführt werden können.…”

Section: Aptamere Und Ihre Modifizierung Für Diagnostische Zweckeunclassified

Die chemische Biologie von Aptameren

Mayer

2009

Angewandte Chemie

View full text Add to dashboard Cite

An einem Strang: Aptamere, kurze einzelsträngige Oligonucleotide mit klar definierter 3D‐Faltung, sind hoch affin und spezifisch für ihre Zielstrukturen und hemmen deren biologische Funktionen. Aptamere können chemisch und/oder enzymatisch hergestellt werden und lassen sich daher als chemische wie auch als biologische Substanzen einordnen. Der aktuelle Stand und neue Entwicklungen auf diesem Gebiet werden vorgestellt.magnified imageAptamere sind kurze einzelsträngige Nucleinsäuren mit einer klar definierten dreidimensionalen Faltung. Sie zeigen eine hohe Affinität und Spezifität für ihre Zielstrukturen und inhibieren deren biologische Funktionen. Aptamere gehören zur Stoffklasse der Nucleinsäuren und können entweder chemisch, enzymatisch oder durch Kombination beider Methoden hergestellt werden. Daher können Aptamere sowohl als chemische wie auch als biologische Substanzen eingeordnet werden. Etablierte Methoden ebenso wie neue Entwicklungen auf dem Gebiet der Aptamere sollen in diesem Aufsatz zusammengefasst werden. Die Verwendung von Aptameren in der chemischen Biologie wird diskutiert.

show abstract

“…The aqueous phase was extracted with Et 2 O (4ϫ 2 mL), and the combined organic phases were washed with water. After drying (Na 2 SO 4 ) and concentration, the crude product was purified by flash chromatography (Et 2 O/petroleum ether, 2:1) to give compound 10a (47 , 2 H, 5a-H, 5b-H , 1 H, 5-H), 3.63 (m, 2 H, 6a-H, 6b-H [50] (0.53 mmol, 100 mg) in toluene (5 mL). The resulting mixture was then stirred at 0°C and Cu 2 O (0.53 mmol, 1.22 mg) was added.…”

Section: 6-dideoxy-3-o-methyl-d-xylo-hexono-14-lactone (8b)mentioning

confidence: 99%

“…After stirring at this temperature for 0.5 h, a solution of 1-[(tert-butyldimethylsilyl)oxy]propan-2-one [50] (100 mg, 0.53 mmol) in THF (3 mL) was added. The resulting mixture was stirred at -78°C for 1 h and then at room temp.…”

Section: Ethyl 4-[(tert-butyldimethylsilyl)oxy]-2-formamido-3-hydroxymentioning

confidence: 99%

Multi‐Step Synthesis and Biological Evaluation of Analogues of Insulin Secretagogue (2S,3R,4S)‐4‐Hydroxyisoleucine

Lajoix¹,

Gross²,

Praly³

2008

Eur J Org Chem

View full text Add to dashboard Cite

A series of stereochemically defined analogues of (2S,3R,4S)‐4‐hydroxyisoleucine and related α‐hydroxy acids have been prepared by multi‐step routes from D‐glucose, whereas ketolization between TBDMS‐protected hydroxypropanone and ethyl isocyanoacetate led to racemic analogues. Bioassays showed that of eight newly synthesized compounds, two of them presented an interesting statistical trend to increase glucose‐induced insulin secretion when tested in isolated rat pancreatic islets in the presence of 8.3 mM glucose and at a concentration of 200 μM, which has previously been shown to be effective for (2S,3R,4S)‐4‐hydroxyisoleucine.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)

show abstract

A ribozyme with michaelase activity

Cited by 57 publications

References 47 publications

Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: synthesis and evaluation in cellulo

Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: synthesis and evaluation in cellulo

Die chemische Biologie von Aptameren

Multi‐Step Synthesis and Biological Evaluation of Analogues of Insulin Secretagogue (2S,3R,4S)‐4‐Hydroxyisoleucine

Contact Info

Product

Resources

About