A rice jacalin‐related mannose‐binding lectin gene, <i>Os<scp>JRL</scp></i>, enhances <i>Escherichia coli</i> viability under high salinity stress and improves salinity tolerance of rice

He, Xiang; Li, L.; Xi, Jing; Cao, Xufeng; Xu, Hengyong; Rong, Songhao; Dong, Yilun; Wang, C.; Chen, R.; Xu, Jin-Quan; Gao, Xiaoling; Xu, Zhengjun

doi:10.1111/plb.12514

Cited by 50 publications

(43 citation statements)

References 68 publications

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“…Recently, this gene was identified in Saltol-1, a major quantitative trait loci related to salt stress [139]. Overexpression of Orysata , also known as SALT, in rice plants improves salinity tolerance [140] and enhances resistance to Magnaporthe grisea infection [141]. The Arabidopsis-related JAX1 was shown to confer broad but specific resistance to potex viruses [142].…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics

Holle

Schutter

Eggermont

et al. 2017

IJMS

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Lectins are present throughout the plant kingdom and are reported to be involved in diverse biological processes. In this study, we provide a comparative analysis of the lectin families from model species in a phylogenetic framework. The analysis focuses on the different plant lectin domains identified in five representative core angiosperm genomes (Arabidopsis thaliana, Glycine max, Cucumis sativus, Oryza sativa ssp. japonica and Oryza sativa ssp. indica). The genomes were screened for genes encoding lectin domains using a combination of Basic Local Alignment Search Tool (BLAST), hidden Markov models, and InterProScan analysis. Additionally, phylogenetic relationships were investigated by constructing maximum likelihood phylogenetic trees. The results demonstrate that the majority of the lectin families are present in each of the species under study. Domain organization analysis showed that most identified proteins are multi-domain proteins, owing to the modular rearrangement of protein domains during evolution. Most of these multi-domain proteins are widespread, while others display a lineage-specific distribution. Furthermore, the phylogenetic analyses reveal that some lectin families evolved to be similar to the phylogeny of the plant species, while others share a closer evolutionary history based on the corresponding protein domain architecture. Our results yield insights into the evolutionary relationships and functional divergence of plant lectins.

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Section: Resultsmentioning

confidence: 99%

Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics

Holle

Schutter

Eggermont

et al. 2017

IJMS

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“…The coding region of OsWRKY97 was amplified and cloned into the pAcGFP1-N1 vector to generate the OsWRKY97-GFP (green fluorescent protein) fusion construct, which was inserted into the pCAMBIA1300 vector, and the constructed vector was transferred into Agrobacterium. After sequencing confirmation, the fusion structure and the control (empty body) vector were cotransfected with another NLS-RFP vector respectively into tobacco cells by Agrobacterium mediated transient expression method, and the fluorescence signal was observed by confocal laser scanning microscope [58].…”

Section: Subcellular Localization Of Oswrky97mentioning

confidence: 99%

Overexpressing the WRKY transcription factor OsWRKY97 improves drought tolerance in rice

Hou

et al. 2020

Preprint

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Background: WRKY transcription factor (TF) is one of the largest TF families in plants and plays an important role in plant development and stress protection. However, the function of individual WRKY gene in plants are still under investigation. Here, we identified a new member of WRKY TF family, OsWRKY97 , and analyze its role in stress resistance by using a series of transgenic plant lines.Results: OsWRKY97 , which positively regulates drought tolerance in rice. OsWRKY97 was expressed in all examined tissues and could be induced by various abiotic stresses. OsWRKY97 is located in the nucleus. Compared with the wild-type (WT), OsWRKY97 -overexpressing (OE) plants enhanced the tolerance of rice to drought and osmotic stress. In addition, OE plants also showed higher sensitivity to exogenous abscisic acid (ABA). When exposed to drought stress, up-regulation OsWRKY97 increased the accumulation of ABA and reduced water loss. Furthermore, OE plants achieved higher proline content and reduced levels of malondialdehyde (MDA) and reactive oxygen species (ROS). The homeostasis of ROS was maintained via the activity of active oxygen scavenging enzyme, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), activated by OsWRKY97Conclusions: These results indicate that OsWRKY97 plays a crucial role in the response to drought stress and may possess high potential value in improving drought tolerance in rice.

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“…Involvement of lectin protein has been reported in regulating heat stress in Dolichos biflorus (Spadoro-Tank and Etzler, 1988). Recently, emerging evidences have highlighted their regulatory role in abiotic stress responses including drought and salinity tolerance (Hirano et al, 2000; Jiang et al, 2012a; He et al, 2016). In plants, 12 lectin protein families have been reported, out of which most of the members are expressed constitutively and show a positive regulatory role against pathogen attack (Jiang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Osr40g3 imparts salt tolerance by regulating GF14e-mediated gibberellin metabolism to activate EG45 in rice

Roy

Sahid

Datta³

et al. 2020

Preprint

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While the role of OsR40 family of lectin proteins in osmotic stress has long been known, their mechanism of stress tolerance has not yet been explored. Among them, the expression of Osr40g3 was most strongly induced in response to salt stress. Here, we report that Osr40g3 plays a dual role in regulating salinity tolerance as well as seed development in rice. The overexpression of Osr40g3 under control of a constitutive promoter significantly improved salinity tolerance but impaired seed development in transgenic rice. Remarkably, transgenicArabidopsis ectopically expressing Osr40g3 exhibited enhanced salinity tolerance but produced seeds normally. It was further confirmed that this Osr40g3-mediated impairment in seed development was due to pollen sterility in the transgenic rice while the pistils remained unaffected. The gene further displayed a precise tissue-specific expression pattern which was essential for proper seed development in rice. The constitutive overexpression led to the interaction of Osr40g3 with OsGF14e in the stamens presumably repressing it which led to pollen sterility. This interaction was, however, highly specific due to the structural distinctiveness of OsGF14e making the phenomenon rice-specific. Nevertheless, in salinity stressed leaves, Osr40g3 interacted with OsEG45 or its ortholog thus leading to salinity tolerance in both rice and Arabidopsis.

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A rice jacalin‐related mannose‐binding lectin gene, OsJRL, enhances Escherichia coli viability under high salinity stress and improves salinity tolerance of rice

Cited by 50 publications

References 68 publications

Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics

Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics

Overexpressing the WRKY transcription factor OsWRKY97 improves drought tolerance in rice

Osr40g3 imparts salt tolerance by regulating GF14e-mediated gibberellin metabolism to activate EG45 in rice

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