A rich mosaic of resistance in extended-spectrum β-lactamase-producing Escherichia coli isolated from red foxes (Vulpes vulpes) in Poland as a potential effect of increasing synanthropization

Osińska, Marcelina; Nowakiewicz, Aneta; Zięba, Przemysław; Gnat, Sebastian; Łagowski, Dominik; AleksandraTrościańczyk,

doi:10.1016/j.scitotenv.2021.151834

Cited by 8 publications

(5 citation statements)

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“…Moreover, this phenomenon was identified mainly in clinical isolates [51,52]. The coexistence of ESBL and PMQR genes among E. coli strains isolated from a wastewater treatment plant and red foxes was also recently reported by Osinska A et al [53] and Osinska M et al [54]. Except for one investigation of 70 E. coli isolates reporting four positives for qnrS1 and qnrB19 (n = 1) [55], no other study has characterized strains collected from wild birds for PMQR determinants.…”

Section: Discussionmentioning

confidence: 71%

High Prevalence of Plasmid-Mediated Quinolone Resistance among ESBL/AmpC-Producing Enterobacterales from Free-Living Birds in Poland

Furmanek-Blaszk

Sektas

2023

IJMS

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In this study, we investigated the occurrence of plasmid-mediated quinolone resistance (PMQR) in extended-spectrum β-lactamase- (ESBL) and/or AmpC-type β-lactamase-producing Enterobacterales isolates from free-living birds in Poland. The prevalence of the qnrB19 gene was 63%, and the distribution of isolates in terms of bacterial species was as follows: 67% (22/33) corresponded to Escherichia coli, 83% (5/6) to Rahnella aquatilis, 44% (4/9) to Enterobacter cloacae and 33% (1/3) to Klebsiella pneumoniae. The qnrB19 gene was also found in a single isolate of Citrobacter freundii. The molecular characteristics of qnrB19-positive isolates pointed to extended-spectrum beta lactamase CTX-M as the most prevalent one (89%) followed by TEM (47%), AmpC (37%) and SHV (16%). This study demonstrates the widespread occurrence of PMQR-positive and ESBL/AmpC-producing Enterobacterales isolates in fecal samples from wild birds. In this work, plasmid pAM1 isolated from Escherichia coli strain SN25556 was completely sequenced. This plasmid is 3191 nucleotides long and carries the qnrB19 gene, which mediates decreased susceptibility to quinolones. It shares extensive homology with other previously described small qnrB19-harboring plasmids. The nucleotide sequence of pAM1 showed a variable region flanked by an oriT locus and a Xer recombination site. The presence of a putative recombination site was detected, suggesting that interplasmid recombination events might have played a role in the development of pAM1. Our results highlight the broad geographical spread of ColE-type Qnr resistance plasmids in clinical and environmental isolates of Enterobacterales. As expected from the results of phenotypic susceptibility testing, no resistance genes other than qnrB19 were identified.

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Section: Discussionmentioning

confidence: 71%

High Prevalence of Plasmid-Mediated Quinolone Resistance among ESBL/AmpC-Producing Enterobacterales from Free-Living Birds in Poland

Furmanek-Blaszk

Sektas

2023

IJMS

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“…The present study detected 28.6% ESBL E. coli in common teals ( Anas crecca ). Previous studies indicated a variable prevalence of ESBL-producing bacteria in ducks: 0% in mallards ( Anas platyrhynchos ) in the Czech Republic [ 38 ] and 33.3% (1/3) in mallards in Poland [ 39 ]; 31% ESBL E. coli were reported from multiple migratory bird species, including common teals ( Anas crecca ) in Pakistan with bla TEM dominant gene type [ 40 ] and 47% in ducks in Sweden with bla CTX-M-15 as the dominant ARG [ 41 ]. In 2017, the first preliminary report was published that provided evidence of the existence of ESBL-producing Klebsiella pneumoniae in wild migratory birds in wetlands in Pakistan [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Detection and Phylogenetic Analysis of Extended-Spectrum β-Lactamase (ESBL)-Genetic Determinants in Gram-Negative Fecal-Microbiota of Wild Birds and Chicken Originated at Trimmu Barrage

Saeed,

Khan,

Ehtisham-ul-Haque

et al. 2023

Antibiotics

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Extended-spectrum β-lactamases (ESBL) give rise to resistance against penicillin and cephalosporin antibiotics in multiple bacterial species. The present study was conducted to map genetic determinants and related attributes of ESBL-producing bacteria in three wild aquatic bird species and chickens at the “Trimmu Barrage” in district Jhang, Punjab province, Pakistan. To study the prevalence of ESBL-producing bacteria, a total of 280 representative samples were collected from wild bird species; cattle egrets (Bubulcus ibis), little egrets (Egretta garzetta) and common teals (Anas crecca) as well as from indigenous chickens (Gallus gallus domesticus) originating from a local wet market. The isolates were confirmed as ESBL producers using a double disc synergy test (DDST) and bacterial species were identified using API-20E and 20NE strips. A polymerase chain reaction (PCR) was used to detect ESBL genetic determinants and for genus identification via 16S rRNA gene amplification. A phenotypic antimicrobial susceptibility test was performed for ESBL-producing isolates against 12 clinically relevant antibiotics using the Kirby–Bauer disk diffusion susceptibility test. A phylogenetic tree was constructed for the sequence data obtained in this study and comparative sequence data obtained from GenBank. The overall prevalence of ESBL-producing bacteria was 34.64% (97/280). The highest percentage (44.28%; 31/70) of ESBL-producing bacteria was recovered from chickens (Gallus gallus domesticus), followed by little egrets (Egretta garzetta) (41.43%; 29/70), common teal (Anas crecca) (28.57%; 20/70) and cattle egrets (Bubulcus ibis) (24.28%; 17/70). Five different ESBL-producing bacteria were identified biochemically and confirmed via 16S rRNA gene sequencing, which included Escherichia coli (72; 74.23%), Enterobacter cloacae (11; 11.34%), Klebsiella pneumoniae (8; 8.25%), Salmonella enterica (4; 4.12%) and Pseudomonas aeruginosa (2; 2.06%). Based on PCR, the frequency of obtained ESBL genes in 97 isolates was blaCTX-M (51.55%), blaTEM (20.62%), blaOXA (6.18%) and blaSHV (2.06%). In addition, gene combinations blaCTX-M + blaTEM, blaTEM + blaOXA and blaCTX-M + blaSHV were also detected in 16.49%, 2.06% and 1.03% of isolates, respectively. The ESBL gene variation was significant (p = 0.02) in different bacterial species while non-significant in relation to different bird species (p = 0.85). Phylogenetic analysis of amino acid sequence data confirmed the existence of CTX-M-15 and TEM betalactamases. The average susceptibility of the antibiotics panel used was lowest for both Klebsiella pneumoniae (62.5% ± 24.42) and Salmonella enterica (62.5% ± 31.08) as compared to Enterobacter cloacae (65.90% ± 21.62), Pseudomonas aeruginosa (70.83% ± 33.42) and Escherichia coli (73.83% ± 26.19). This study provides insight into the role of aquatic wild birds as reservoirs of ESBL-producing bacteria at Trimmu Barrage, Punjab, Pakistan. Hence, active bio-surveillance and environment preservation actions are necessitated to curb antimicrobial resistance.

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“…using cephalosporin-additive or the specific-selective agars) to investigate the prevalence of ESC resistance in E. coli isolated from terrestrial wild animals worldwide, including macaque (0%) and wild boars (33.0%) in Algeria [19]; wild boars (4.5%) in Spain [20]; various wild mammals (1.5%) in France [21]; wild boars (1.2%) and ruminants (1.1%) in Germany [22]; wild boars (23.3%) in Italy [23]; badger (8.9%) and fox (11.53%) in Ireland [24]; red foxes (10.5%) and martens (7.5%) in Poland [25]; wild boars (14%) in Tunisia [26]; and raccoons (56.9%) in the United States [9]]. These results implied that the prevalence of ESC-resistant E.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have been conducted in a similar manner [i.e. using cephalosporin-additive or the specific-selective agars) to investigate the prevalence of ESC resistance in E. coli isolated from terrestrial wild animals worldwide, including macaque (0%) and wild boars (33.0%) in Algeria [19]; wild boars (4.5%) in Spain [20]; various wild mammals (1.5%) in France [21]; wild boars (1.2%) and ruminants (1.1%) in Germany [22]; wild boars (23.3%) in Italy [23]; badger (8.9%) and fox (11.53%) in Ireland [24]; red foxes (10.5%) and martens (7.5%) in Poland [25]; wild boars (14%) in Tunisia [26]; and raccoons (56.9%) in the United States [9]]. These results implied that the prevalence of ESC-resistant E. coli isolates from wild animals is greatly different by animal species, which is closely related to their habitat and/or feeding habit and country, and Japanese raccoon dogs are comparable to other terrestrial wild animals in terms of ESC-resistant E. coli reservoir.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance in Escherichia coli isolates from Japanese raccoon dogs (Nyctereutes viverrinus) in Kanagawa Prefecture, Japan: Emergence of extended-spectrum cephalosporin-resistant human-related clones

Shimizu

Kido²,

Miyashita

et al. 2022

Journal of Medical Microbiology

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Introduction. Wild animals are one of the putative reservoirs of antimicrobial-resistant bacteria, but the significance of raccoon dogs remains to be investigated. Hypothesis. Raccoon dogs can be a reservoir of antimicrobial-resistant bacteria. Aim. This study aimed to explore the prevalence of antimicrobial resistance, mainly extended-spectrum cephalosporins resistance, in Escherichia coli isolates from faeces of 80 Japanese raccoon dogs in Kanagawa Prefecture, Japan. Methodology. All of the 80 faecal samples were streaked onto deoxycholate-hydrogen sulfate-lactose (DHL) and cefotaxime (CTX)-supplemented DHL (DHL-CTX) agars. Susceptibilities to ten antimicrobials were determined using the agar dilution method. Additionally, extended-spectrum β-lactamases (ESBLs) and AmpC-type β-lactamases (ABLs) were identified in addition to sequence types (STs), in ESC-resistant isolates by a polymerase chain reaction and sequencing. Results. Out of all the samples, 75 (93.8 %) and 20 (25.0 %) E. coli isolates were isolated by DHL and DHL-CTX agars, respectively. Significantly higher resistance rates to most of the drugs were found in DHL-CTX-derived isolates than DHL-derived isolates (P<0.01). Genetic analysis identified CTX-M-14 (n=6), CTX-M-2 (n=2), CTX-M-1 (n=1) and CTX-M-55 (n=1) as ESBLs, and CMY-2 (n=8) and DHA-1 (n=1) as ABLs in 20 DHL-CTX-derived isolates. Most of the detected STs were related to Japanese humans (i.e. ST10, ST58, ST69, ST131, ST357, ST648 and ST4038). Notably, this is the first report on ST69, ST131, ST155 and ST648, which are well-known international high-risk clones in Japanese raccoon dogs. Conclusion. Our findings underscore the need to understand the significance of raccoon dogs as an antimicrobial-resistant bacteria reservoir using one health approach.

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A rich mosaic of resistance in extended-spectrum β-lactamase-producing Escherichia coli isolated from red foxes (Vulpes vulpes) in Poland as a potential effect of increasing synanthropization

Cited by 8 publications

References 84 publications

High Prevalence of Plasmid-Mediated Quinolone Resistance among ESBL/AmpC-Producing Enterobacterales from Free-Living Birds in Poland

High Prevalence of Plasmid-Mediated Quinolone Resistance among ESBL/AmpC-Producing Enterobacterales from Free-Living Birds in Poland

Detection and Phylogenetic Analysis of Extended-Spectrum β-Lactamase (ESBL)-Genetic Determinants in Gram-Negative Fecal-Microbiota of Wild Birds and Chicken Originated at Trimmu Barrage

Antimicrobial resistance in Escherichia coli isolates from Japanese raccoon dogs (Nyctereutes viverrinus) in Kanagawa Prefecture, Japan: Emergence of extended-spectrum cephalosporin-resistant human-related clones

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