A Robust Au−C≡C Functionalized Surface: Toward Real‐Time Mapping and Accurate Quantification of Fe²⁺ in the Brains of Live AD Mouse Models

Abstract

Described here is that AuÀCCb onds showed the highest stability under biological conditions,w ith abundant thiols,a nd the best electrochemical performance compared to Au À Sand Au À Se bonds.The new finding was also confirmed by theorical calculations.B ased on this finding, as pecific molecule for recognition of Fe 2+ was designed and synthesized, and used to create as elective and accurate electrochemical sensor for the quantification of Fe 2+ .T he present ratiometric strategy demonstrates high spatial resolution for real-time tracking of Fe 2+ in ad ynamic range of 0.2-120 mM. Finally, amicroelectrode arraywith good biocompatibility was applied in imaging and biosensing of Fe 2+ in the different regions of live mouse brains.U sing this tool, it was discovered that the uptake of extracellular Fe 2+ into the cortex and striatum was largely mediated by cyclic adenosine monophosphate (cAMP) through the CREB-related pathway in the brain of am ouse with Alzheimersd isease.

Spectroscopic Evidence for a Covalent Sigma Au–C Bond on Au Surfaces Using 13C Isotope Labeling

“…In spite of the intense research interest in the Au–C bonding on Au surfaces, direct experimental evidence of its existence is still missing to support the calculations of its nature. 22 , 23 X-ray photoelectron spectroscopy (XPS) is typically applied to investigate interactions for modified metal surfaces, including Au. Laforgue et al.…”

A Highly Stable Two-Photon Ratiometric Fluorescence Probe for Real-Time Biosensing and Imaging of Nitric Oxide in Brain Tissues and Larval Zebrafish

“…Moreover, the synthesized AuNCs displayed a board absorption and apparent TPF emission around 540 nm (Supporting information Figure S10). Then, a ratiometric TPF probe was assembled by conjugating RBD onto AuNCs through robust Au-COC bond, 35 named as RBD@AuNCs. Fourier transform infrared spectrometer (FT-IR) spectrum of RBD@AuNCs exhibited the disappearance of typical vibration peaks of O (3293 cm -1 ) and O $!…”

Nano- and Microsensors for In Vivo Real-Time Electrochemical Analysis: Present and Future Perspectives

“…Over the past few years, several articles have considered ratiometric sensors for use in in vivo measurement [ 119 , 120 , 121 , 122 ]. The first study demonstrated changes in pH and compared the quantity of copper ions in normal and Alzheimer’s disease (AD) rats [ 119 ].…”

“…Other metal ions are also involved in the processes of neurodegenerative diseases. Zhang et al presented a ratiometric electrochemical sensor based on a Au−C≡C bond surface for real-time mapping and accurate quantification of Fe 2+ in the brains of a live AD mouse [ 122 ]. The author used a carbon CF microelectrode as a base followed by gold precipitation on the electrode surface.…”