The complexity of the user interfaces and the operating modes present in numerous assistive devices, such as intelligent prostheses, influence patients to shed them from their daily living activities. A methodology to evaluate how diverse aspects impact the workload evoked when using an upper-limb bionic prosthesis for unilateral transradial amputees is proposed and thus able to determine how user-friendly an interface is. The evaluation process consists of adapting the same 3D-printed terminal device to the different user-prosthesis-interface schemes to facilitate running the tests and avoid any possible bias. Moreover, a study comparing the results gathered by both limb-impaired and healthy subjects was carried out to contrast the subjective opinions of both types of volunteers and determines if their reactions have a significant discrepancy, as done in several other studies.