A role for cadherins in tissue formation

Larue, Lionel; Antos, Christopher L.; Butz, Stefan; Huber, Otmar; Delmas, Véronique; Dominis, Mara; Kemler, Rolf

doi:10.1242/dev.122.10.3185

Cited by 321 publications

(37 citation statements)

References 48 publications

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“…Firstly, Ecadherin null ES cells, unlike their wild-type counterparts, are incapable of differentiating in vitro and in vivo into derivatives of all three germ-layers. 179 When ES cells are injected under the kidney capsule or subcutaneously for teratoma formation, Ecadherin null ES cells do not form organised structures, whereas teratomas of wild-type ES cells are composed of completely differentiated cells of all three germ-layers. 179 The phenotype of Ecadherin null ES cells can be rescued by constitutive expression of Ecadherin or Ncadherin after transfection.…”

Section: Function Of Cadherins During Embryogenesismentioning

confidence: 99%

Cadherins in development and cancer

2008

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Proper embryonic development is guaranteed under conditions of regulated cell-cell and cell-matrix adhesion. The cells of an embryo have to be able to distinguish their neighbours as being alike or different. Cadherins, single-pass transmembrane, Ca(2+)-dependent adhesion molecules that mainly interact in a homophilic manner, are major contributors to cell-cell adhesion. Cadherins play pivotal roles in important morphogenetic and differentiation processes during development, and in maintaining tissue integrity and homeostasis. Changes in cadherin expression throughout development enable differentiation and the formation of various organs. In addition to these functions, cadherins have strong implications in tumourigenesis, since frequently tumour cells show deregulated cadherin expression and inappropriate switching among family members. In this review, I focus on E- and N-cadherin, giving an overview of their structure, cellular function, importance during development, role in cancer, and of the complexity of Ecadherin gene regulation.

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Section: Function Of Cadherins During Embryogenesismentioning

confidence: 99%

Cadherins in development and cancer

2008

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“…E-Cadherin, a member of the Cadherin-superfamily, is a calcium-dependent transmembrane glycoprotein, first described in 1977 by Takeichi [ 9 ]. A type-1-cadherine, E-Cadherin has multiple roles in physiological as well as pathological processes of cell migration and invasion, such as embryonic development [ 10 ], tissue morphogenesis [ 11 ] and cell–cell adhesion between neighbouring epithelial cells [ 12 ]. The role of E-Cadherin in cancer progression is established and well documented [ 13 – 16 ], represented by repression of E-Cadherin expression at the primary tumour site [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

The role of E-Cadherin expression in primary site of breast cancer

Karsten¹,

Kolben²,

Mahner³

et al. 2021

Arch Gynecol Obstet

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Purpose The tumour’s ability to metastasize is the major cause for fatal outcomes in cancer diseases. In breast cancer, aberrant E-Cadherin expression has been linked to invasiveness and poor prognosis. Method We assessed expression of E-Cadherin by immunohistochemistry in primary tumour tissue from 125 female breast cancer patients. Staining intensities were analysed using the immunoreactive score (IRS). We investigated E-Cadherin expression and its associations with clinicopathological parameters (age, tumour size, lymph node status, grade, hormone receptors, Her2 Status) as well as with recurrence and survival. Results Increased, rather than aberrant E-Cadherin expression was found and was associated with poor outcome (p = 0.046). Our data show an association between elevated E-Cadherin in primary tumour tissue and an unfavourable negative prognosis in patients. Conclusion This association was somehow unexpected as loss of E-Cadherin has long been regarded as a prerequisite for development of invasiveness and metastases. Our findings support the notion that E-Cadherin promotes, rather than suppresses, development of metastasis and invasiveness.

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“…Since E-Cadherin is the predominant adhesion molecule in non-neural epithelia, we hypothesised that it might be important in AMIS localisation. To achieve a full removal of E-cadherin, we employed an E-Cadherin knock-out ( Cdh1 KO) mESC line (Larue et al, 1996). Whilst E-Cadherin expression was lost in Cdh1 KO cells, they maintained P-Cadherin expression and were still able to form cell clusters when cultured in Matrigel (Figure S2A, B).…”

Section: Resultsmentioning

confidence: 99%

E-cadherin mediated Apical Membrane Initiation Site localisation

Liang

Weberling

Hii

et al. 2021

Preprint

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Individual cells within de novo polarising tubes and cavities must integrate their forming apical domains into a centralised apical membrane initiation site (AMIS). This is necessary to enable organised lumen formation within multi-cellular tissue. Despite the well documented importance of cell division in localising the AMIS, we have found a division-independent mechanism of AMIS localisation that relies instead on CADHERIN-mediated cell-cell adhesion. Our study of de novo polarising mESCs suggest that cell-cell adhesion directs the localisation of apical proteins such as PAR-6 to a centralised AMIS. Unexpectedly, we also found that mESC cell clusters lacking functional E-CADHERIN were still able to form a lumen-like cavity in the absence of AMIS localisation and did so at a later stage of development via a ‘closure’ mechanism, instead of via hollowing. This work suggests that there are two, interrelated mechanisms of apical polarity localisation: cell adhesion and cell division. Alignment of these mechanisms allows for redundancy in the system and ensures the localisation of a coherent epithelial structure within a growing organ.

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A role for cadherins in tissue formation

Cited by 321 publications

References 48 publications

Cadherins in development and cancer

Cadherins in development and cancer

The role of E-Cadherin expression in primary site of breast cancer

E-cadherin mediated Apical Membrane Initiation Site localisation

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