A role for interleukin-1 in epidermal differentiation: Regulation by expression of functional versus decoy receptors

Eller, Mark S.; Yaar, Mina; Ostrom, Kristin; Harkness, Daniel D; Gilchrest, Barbara A.

doi:10.1242/jcs.108.8.2741

Cited by 39 publications

(2 citation statements)

References 40 publications

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“…IL‐1α, a proinflammatory cytokine capable of inducing neutrophil and macrophage recruitment, is accounted for the vast majority of epidermal‐associated IL‐1 activity 69 . Overexpression of IL‐1α is positively correlated with reduced SCH as well as symptom exacerbation in many skin diseases 70,71 .…”

Section: Discussionmentioning

confidence: 99%

“…IL‐1α, a proinflammatory cytokine capable of inducing neutrophil and macrophage recruitment, is accounted for the vast majority of epidermal‐associated IL‐1 activity. 69 Overexpression of IL‐1α is positively correlated with reduced SCH as well as symptom exacerbation in many skin diseases. 70 , 71 Our result suggest that, the treatment might have reduced possible subclinical inflammation induced by dry skin, as the aged skin may exhibits signs of continuous inflammation.…”

Section: Discussionmentioning

confidence: 99%

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Effects of a leave‐on product on the strength of the dermoepidermal junction: An exploratory, intraindividual, randomized controlled trial in older adults with dry skin

Amin,

Rancan,

Hillmann

et al. 2024

Health Science Reports

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Background and AimsSkin aging is associated with dry skin and a decrease of the strength of the dermoepidermal adhesion, which increases the risk for lacerations (skin tears). Application of leave‐on products improves dry skin and seems to reduce skin tear incidence. The aim of this study was to measure the effects of a humectant containing leave‐on product on the strength of the dermoepidermal junction in older adult participants with dry skin.MethodsA randomized controlled trial using a split body design was conducted. One forearm was randomly selected and treated with a lipophilic leave‐on product containing 5% urea for 8 weeks. The other forearm was the control. The parameters stratum corneum hydration (SCH), transepidermal water loss, pH, roughness, epidermal thickness and skin stiffness were measured at the baseline, Weeks 4 and 8. At Week 8, suction blisters were created and time to blistering was measured. Blister roofs and interstitial fluid were analyzed for Interleukin‐1α, 6 and 8.ResultsTwelve participants were included. After 8 weeks treatment, SCH was higher (median difference 11.6 AU), and the overall dry skin score (median difference −1) and median roughness (Rz difference −12.2 µm) were lower compared to the control arms. The median group difference for Interleukin‐1α was −452 fg/µg total protein (TP) in the blister roofs and −2.2 fg/µg TP in the blister fluids. The median time to blister formation was 7.7 min higher compared to the control arms.ConclusionThe regular application of humectant containing leave‐on products improves dry skin and seems to lower inflammation and contribute to the strengthening of the dermoepidermal adhesion. This partly explains how the use of topical leave‐on products helps to prevent skin tears.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of a leave‐on product on the strength of the dermoepidermal junction: An exploratory, intraindividual, randomized controlled trial in older adults with dry skin

Amin,

Rancan,

Hillmann

et al. 2024

Health Science Reports

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Induction of interleukin (IL)-1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain-induced keratinocyte proliferation and morphological change

Takei

Konishi

et al. 1998

J. Cell. Biochem.

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Recent studies in our laboratory have demonstrated that mechanical strain alters many facets of keratinocyte biology including proliferation, protein synthesis, and morphology. IL-1 is known to play an important role in the autocrine regulation of these basic cellular properties under basal and stimulated conditions. However, it is not known whether IL-1 plays a role in strain-induced alteration of keratinocyte biology. Thus, the objective of this study was to test the hypothesis that cyclic strain stimulates IL-1 expression and that strain-induced changes in keratinocyte function is regulated by IL-1. To test this hypothesis, we examined the effect of cyclic strain (10% average deformation) on keratinocyte IL-1 gene expression and the effect of neutralizing antibodies of IL-1 alpha and IL-1 beta on strain-induced changes in keratinocyte proliferation, morphology, and orientation. Northern blot analyses demonstrated that steady state levels of IL-1 alpha and beta mRNA were elevated by 4 h, peaked at 1 2 h of cyclic strain (IL-1 alpha, 304+/-14.2%; IL-1 beta, 212+/-5.6% increase vs. static controls) and decreased gradually by 24 h. IL-1 antibodies (IL-1 alpha, 0.01 microg/ml; IL-1 beta, 0.01 microg/ml) significantly blocked strain-induced keratinocyte proliferation as well as the basal rate of proliferation. In contrast, IL-1 antibodies (IL-1 alpha, 0.01 microg/ml; IL-1 beta, 0.1 microg/ml) had no effect on strain-induced morphological changes such as elongation and alignment. We conclude that mechanical strain induces IL-1 mRNA expression in keratinocytes. The role of IL-1 in mediating strain-induced changes in keratinocyte biology remains to be determined but appears to be independent of morphological changes.

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Retinoic acid induces expression of the interleukin‐1β gene in cultured normal human mammary epithelial cells and in human breast carcinoma lines

Liu

Gudas

2002

Journal Cellular Physiology

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Retinoic acid (RA) and its derivatives inhibit the proliferation of normal human mammary epithelial cells (HMEC) and some breast carcinoma lines by mechanisms which are not fully understood. To identify genes that mediate RA-induced cell growth arrest, an HMEC cDNA library was synthesized and subtractive screening was performed. We identified the interleukin-1beta (IL-1beta) gene as an RA induced gene in HMEC. Northern blot analyses showed that the IL-1beta gene was up-regulated as early as 2 h after RA treatment. Results from the treatment of HMEC with cycloheximide and actinomycin D indicated that the regulation of the IL-1beta gene by RA occurred at the transcriptional level and that the IL-1beta gene is a direct, downstream target gene of RA. To evaluate the effects of IL-1beta on cell proliferation, the proliferation of HMEC was measured in the presence of RA or IL-1beta, or both. Either RA or IL-1beta could significantly inhibit the proliferation of HMEC. However, the addition of soluble IL-1 receptor antagonist (sIL-1ra) to the cell culture medium did not block RA-induced HMEC growth inhibition, whereas sIL-1ra did block the growth inhibition of HMEC by IL-1beta. IL-1beta expression was not observed in the three carcinoma cell lines, MCF-7, MDA-MB-231, and MDA-MB-468, as compared to the HMEC. Growth curves of the breast carcinoma cell lines showed strong inhibitory effects of RA and IL-1beta on the growth of the estrogen receptor (ER) positive MCF-7 cell line, but only a small effect on the ER negative MDA-MB-231 cells. The expression of the IL-1beta gene was also transcriptionally activated by RA in normal epithelial cells of prostate and oral cavity. Our results suggest that: (a) the IL-1beta gene is a primary target of RA receptors in HMEC; (b) the enhanced expression of the IL-1beta gene does not mediate the RA-induced growth arrest of HMEC; and (c) the expression of the IL-1beta gene is low or absent in all three human breast carcinoma cell lines examined, but the defect in the IL-1beta signaling pathway may be different in ER positive versus ER negative carcinoma cells.

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A role for interleukin-1 in epidermal differentiation: Regulation by expression of functional versus decoy receptors

Cited by 39 publications

References 40 publications

Effects of a leave‐on product on the strength of the dermoepidermal junction: An exploratory, intraindividual, randomized controlled trial in older adults with dry skin

Effects of a leave‐on product on the strength of the dermoepidermal junction: An exploratory, intraindividual, randomized controlled trial in older adults with dry skin

Induction of interleukin (IL)-1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain-induced keratinocyte proliferation and morphological change

Retinoic acid induces expression of the interleukin‐1β gene in cultured normal human mammary epithelial cells and in human breast carcinoma lines

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