A role of estrogens in the regulation of RNA polymerase in surviving rat uteri

Nicolette, John A.; Lemahieu, M; Mueller, Gerald C.

doi:10.1016/0005-2787(68)90228-1

Cited by 29 publications

(9 citation statements)

References 6 publications

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“…In contrast, McEwen's group (31 ) have demonstrated the pituitary weight and total RNA (both mRNA and rRNA) levels. The estrogen-induced increases in total RNA are in agree-that the levels of E 2 retention, binding capacity and kinetics are similar in the pituitary of female and male rats by simultaneously ment with the classical reports by Gorski and Mueller, that estrogen treatment resulted in a rapid (within 1 h) elevation of measuring absolute levels of the cell nuclear 17b-estradiol retention and cytosol E 2 saturation binding capacities in gonadecto-RNA polymerase activity in the uterus (19,20). Very recently, Ying et al (21) also reported an estrogen-induced rRNA accumu-mized-adrenalectomized animals.…”

Section: Resultssupporting

confidence: 73%

“…This feature is consistent with the fact that estrogen induction of in the males, presence of specific ER repressor and the inability of ER to access the target genes in the males (28). ER are RNA polymerase activity in the rat uterus is dose-dependently blocked by the addition of cycloheximide ( 20). Thus, estrogen absolutely required for the genomic actions of estrogen, and the diÂerence in the levels of ER in some systems may be a factor may increase RNA level through induction of RNA polymerase synthesis.…”

Section: Resultsmentioning

confidence: 99%

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Gender‐Specific Induction of Pituitary RNA by Estrogen and its Modification by Thyroid Hormone

Dellovade

Pfaff

1997

J Neuroendocrinology

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Estrogen and thyroid hormones play important roles in the regulation of pituitary function. We presently show that pituitary weight and total cellular RNA levels were significantly decreased by ovariectomy in female rats and were significantly increased by castration in males, without alterations in pituitary DNA levels as compared to intact animals. Treatment with a single dose of estrogen produced a significant increase in pituitary RNA in ovariectomized females but not castrated males. This effect was more obvious following multiple doses of estrogen, and was blocked by pretreatment with cycloheximide, or surprisingly by concomitant administration of triiodothyronine (T3). Analysis of estrogen response element (ERE) binding activity in pituitary nuclear protein extracts revealed that estrogen produced a rapid induction of a slow mobility complex of ERE binding in ovariectomized females much greater than in castrated males. Thus, estrogen-induced increases in pituitary total RNA levels are dependent on new protein synthesis, are gender-specific, are inhibited by T3, and may be mediated via specific estrogen-induced changes in protein-DNA interactions.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Gender‐Specific Induction of Pituitary RNA by Estrogen and its Modification by Thyroid Hormone

Dellovade

Pfaff

1997

J Neuroendocrinology

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“…However, recent results of Knowler & Smellie (1971) suggest that a high-molecular-weight RNA (non-ribosomal) is synthesized within 30min after administration of oestradiol before the synthesis of ribosomal RNA is stimulated. In studies with isolated nuclei, the administration of oestradiol-17f to ovariectomized rats for 30-60min caused an increase in the endogenous RNA polymerase I activity (Noteboom & Gorski, 1963a;Hamilton et al, 1965Hamilton et al, , 1968Nicolette et al, 1968;Barry & Gorski, 1971;Raynaud-Jammet et al, 1971). No response was observed in the other class of endogenous RNA polymerase activity (II) until 12-24h after the injection of the hormone.…”

mentioning

confidence: 99%

Early effects of oestradiol-17β on the chromatin and activity of the deoxyribonucleic acid-dependent ribonucleic acid polymerases (I and II) of the rat uterus

Glasser

Chytil

Spelsberg

1972

Biochemical Journal

162

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Oestradiol-17beta (1.0mug) was injected intravenously into ovariectomized rats. The earliest detectable hormonal response in isolated uterine nuclei was an increase (10-15min) in RNA polymerase II activity (DNA-like RNA synthesis), which reached a peak at 30min and then decreased to control values (by 1-2h) before displaying a second increase over control activity from 2 to 12h. The next response to oestradiol-17beta was an increase (30-60min) in polymerase I activity (rRNA synthesis) and template capacity of the chromatin. The concentrations of acidic chromatin proteins did not begin to increase until 1h after injection of oestradiol-17beta and histone concentrations showed no significant changes during the 8h period after administration. The early (15min) increase in RNA synthesis in ;high-salt conditions' can be completely eliminated by alpha-amanitin, an inhibitor of the RNA polymerase II. The exact nature of this early increase in endogenous polymerase II activity remains to be determined, e.g. whether it is caused by the increased availability of transcribable DNA of the chromatin or via direct hormonal activation of the enzyme per se.

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“…Indirect evidence suggests that production of messenger RNA for a specific soluble protein in the rat uterus is an especially early event (DeAngelo Sc Gorski 1970, Baulieu et al 1972. It was found that the RNA polymerase activity of nuclei isolated from uteri of immature or castrate rats is increased two-to three-fold by administration of estradiol to the animal one to four hours before the uteri are excised (Gorski 1964, Nicolette et al 1968, Raynaud-Jammet et al 1971, Barry Se Gorski 1971. This effect on RNA polymerase appears to be qualitative as well as quantitative-, nearest neighbor frequency analysis shows that the RNA synthesized by uterine nuclei isolated from estrogen-treated animals contains a higher content of cytidine and guanosine linkages as compared to adenosine and uridine (Barton and Liao 1967, Trachewsky Se Segal 1967, Nicolette Se Babler 1972.…”

Section: Estrogen-receptor Complexes and Their Interrelationmentioning

confidence: 99%