A safety and feasibility study with platelet lysate expanded bone marrow mesenchymal stromal cells for the treatment of acute graft-versus-host disease in Brazil

Adipocyte Derived Mesenchymal Stromal Cell and Platelet Lysate: Ideal Cell and Supplement for the Treatment of Immune-Inflammatory Diseases?

“…As shown in our previous study, in platelet lysate (PL) supplemented cultures, MSC proliferates significantly better then when cultured with FCS [9]. Along with a higher proliferative index, PL could also alter MSC characteristics and its efficacy, leading to concerns about using fetal bovine serum and other non-human materials, evidence ensuring this is that MSC proliferates better using human derived materials, like platelet lisate (LP).…”

“…PL was produced in house. Briefly, platelets passed through freezing and thawing cycle, centrifuged 4 times at 3000 g for 30 min, sterilized through 0.22 µm filter, heparinized and stored in −20˚C freezer for up to 3 months [9].…”

“…Concerns about xenoreactions to fetal calf serum supplemented cultured cells for cellular therapy, led our group [9] and others [10] to explore human derived supplements. As shown in our previous study, in platelet lysate (PL) supplemented cultures, MSC proliferates significantly better then when cultured with FCS [9].…”

The Effect of Human Platelet-Rich Plasma on Adipose-Derived Stem Cell Proliferation and Osteogenic Differentiation

“…Because of their immunosuppressory and engraftment-promoting properties, MSC have been used routinely in the clinical settings both to facilitate hematopoietic recovery and to treat steroid-resistant acute graft-versus-host disease [2]. Compared to other types of stem cells, MSC are considered as a useful cell source in tissue engineering owing to their high capacity for proliferation and differentiation [1, 3].…”

“…Following promising results of mesenchymal stem cells (MSC) in experimental studies and clinical trials, these cells have been recently presented as a candidate for cell-based therapy in solid organ transplantation and repair of damaged tissues such as bone, cartilage, muscle, ligament, tendon, and adipose [ 1 ]. Because of their immunosuppressory and engraftment-promoting properties, MSC have been used routinely in the clinical settings both to facilitate hematopoietic recovery and to treat steroid-resistant acute graft-versus-host disease [ 2 ]. Compared to other types of stem cells, MSC are considered as a useful cell source in tissue engineering owing to their high capacity for proliferation and differentiation [ 1 , 3 ].…”