A salivary factor binds a cuticular protein and modulates biting by inducing morphological changes in the mosquito labrum

Arnoldi, Irene; Mancini, Giulia F.; Fumagalli, Marco; Gastaldi, Dario; D’Andrea, Luca; Bandi, Claudio; Venere, Monica; Iadarola, Paolo; Forneris, Federico; Gabrieli, Paolo

doi:10.1016/j.cub.2022.06.049

Cited by 11 publications

(7 citation statements)

References 85 publications

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“…Genetic differentiation of the population suggests that the locus was under selection. The reported interaction of this gene with LIPS causes changes in the morphology of the labral cuticle (Arnoldi et al, 2022), which we presume is related to the fact that SansaId colony has significantly shorter tongue lengths than others.…”

Section: Genetic Variation and Environmental Adaptationmentioning

confidence: 75%

Genomic and morphological analyses of Asian honeybee subspecies, Apis cerana sanshasis, suggest it's evolutional origin and adaption to island

Gao

Tang

Zhao

et al. 2022

Preprint

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It’s important to discover new resources and expand knowledge of the distribution range of Apis cerana for the conservation and utilization of genetic resources. Here, we newly sequenced genomes and apply population genetic and morphological analysis methods using publicly available genome-wide datasets to investigate the origin and adaptation of A.cerana sanshasis from Yongxing Island. The results showed SanshaId colony was significantly genetically differentiated from others with an average Fst of 0.2669, indicating a level of subspecies differentiation. Morphological clustering also showed significant differences, especially the tongue length of SanshaId colony was significantly smaller than that of others. In summary, we concluded that A.cerana sanshasis is a subspecies of A.cerana. Population history analysis showed SanshaId population has a recent common ancestral feature with Hainan’s, and subspecies differentiation occurred around 0.57 Ma due to geological movements causing geographic isolation. We examined the genetic variation between the recently marginalized colonies from HainanId and SanshaId, and found high frequency nonsynonymous mutations gene in SanshaId colony involved in Glutathione metabolism and other signaling pathways, among which a gene potentially related to tongue length morphology, Cuticular, was subject to evolutionary selection; meanwhile, differential tissue expression profiles showed a correlation with glucose metabolism genes are highly expressed in the midgut, suggesting these genes may be associated with adaptation to tropical island environments. Our results expand the understanding of the distribution range of Asian honeybee and provide the basis for understanding the population dynamics of A.cerana sanshasis and the molecular evolutionary mechanisms of adaptation to tropical island environments.

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Section: Genetic Variation and Environmental Adaptationmentioning

confidence: 75%

Genomic and morphological analyses of Asian honeybee subspecies, Apis cerana sanshasis, suggest it's evolutional origin and adaption to island

Gao

Tang

Zhao

et al. 2022

Preprint

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“…Thus, we conclude that there are no significant structural changes to AaPC upon binding with DENV E protein; however, it is not clear that any other binding partners would alter the conformation of AaPC. Besides chitin-binding, it was reported that cuticle protein 19 from Culex pipiens (Cp19), an RR-2 protein sharing about 86% sequence similarity with AaPC (Table S1), interacts with the abrum-interacting protein of the saliva 2 (LIPS-2) to induce a change in the morphology of the cuticle and affects mosquito intradermal probing before blood engorgement (Arnoldi et al, 2022). However, whether RR-2 consensus participates in the interaction remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Structural and functional characterization of Aedes aegypti pupal cuticle protein that controls dengue virus infection

Huang,

Gavor,

Tulsian

et al. 2023

Protein Science

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The pupal cuticle protein from Aedes aegypti (AaPC) inhibits dengue virus (DENV) infection; however, the underlying mechanism of this inhibition remains unknown. Here, we report that AaPC is an intrinsically disordered protein and interacts with domain I/II of the DENV envelope protein via residues Asp59, Asp61, Glu71, Asp73, Ser75 and Asp80. AaPC can directly bind to and cause the aggregation of DENV, which in turn blocks virus infection during the virus‐cell fusion stage. AaPC may also influence viral recognition and attachment by interacting with human immune receptors DC‐SIGN and CD4. These findings enhance our understanding of the role of AaPC in mitigating viral infection and suggest that AaPC is a potential target for developing inhibitors or antibodies to control dengue virus infection.This article is protected by copyright. All rights reserved.

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“…Interestingly, beyond aphid acrostyle at the tip of maxillary stylets, a cuticular structure known as V-shaped ridges located at the tip of the stylet fascicle of Aedes mosquitoes was recently shown to exhibit salivary protein-binding capacity [ 44 ]. In their study, the authors demonstrated that LIPS-2, the labrum-interacting protein of the saliva 2 of Aedes mosquitoes, interacts with Cp19, a cuticular protein from the CPR_RR-2 subfamily emerging on the external cuticle of the apical part of the labrum.…”

Section: Discussionmentioning

confidence: 99%

“…LIPS-2 is a female-specific salivary factor shown to have immunogenic properties in humans [ 45 ]. Arnoldi and colleagues characterized a new role for this protein in establishing a feedback signaling pathway in aedines, and further showed that interaction with Cp19 is the initial trigger of this signaling pathway [ 44 ]. Echoing our present study, this article highlights that the cuticular proteins at the tip of piercing-sucking insects can act as a natural platform for key molecular interactions, in this case, with a mosquito salivary protein involved in the initial phases of biting behavior.…”

Section: Discussionmentioning

confidence: 99%

Role of Acrostyle Cuticular Proteins in the Retention of an Aphid Salivary Effector

Deshoux

Monsion

Pichon

et al. 2022

IJMS

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To avoid the activation of plant defenses and ensure sustained feeding, aphids are assumed to use their mouthparts to deliver effectors into plant cells. A recent study has shown that effectors detected near feeding sites are differentially distributed in plant tissues. However, the precise process of effector delivery into specific plant compartments is unknown. The acrostyle, a cuticular organ located at the tip of maxillary stylets that transiently binds plant viruses via its stylin proteins, may participate in this specific delivery process. Here, we demonstrate that Mp10, a saliva effector released into the plant cytoplasm during aphid probing, binds to the acrostyles of Acyrthosiphon pisum and Myzus persicae. The effector probably interacts with Stylin-03 as a lowered Mp10-binding to the acrostyle was observed upon RNAi-mediated reduction in Stylin-03 production. In addition, Stylin-03 and Stylin-01 RNAi aphids exhibited changes in their feeding behavior as evidenced by electrical penetration graph experiments showing longer aphid probing behaviors associated with watery saliva release into the cytoplasm of plant cells. Taken together, these data demonstrate that the acrostyle also has effector binding capacity and supports its role in the delivery of aphid effectors into plant cells.

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A salivary factor binds a cuticular protein and modulates biting by inducing morphological changes in the mosquito labrum

Cited by 11 publications

References 85 publications

Genomic and morphological analyses of Asian honeybee subspecies, Apis cerana sanshasis, suggest it's evolutional origin and adaption to island

Genomic and morphological analyses of Asian honeybee subspecies, Apis cerana sanshasis, suggest it's evolutional origin and adaption to island

Structural and functional characterization of Aedes aegypti pupal cuticle protein that controls dengue virus infection

Role of Acrostyle Cuticular Proteins in the Retention of an Aphid Salivary Effector

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