A Salmonella enterica conjugative plasmid impairs autophagic flux in infected macrophages

Chu, Yuanyuan; Yang, Yanru; Li, Yuanyuan; Ye, Ying; Yan, Jing; Wang, Ting; Wu, Shuyan; Huang, Rui

doi:10.1016/j.micinf.2014.05.001

Cited by 7 publications

(8 citation statements)

References 34 publications

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“…It provided the direct evidence that spvB could inhibit autophagy. In line with this finding, it had been confirmed that the Salmonella virulence plasmid harboring spv genes was involved in blocking the formation of autophagosome in the earlier autophagy process in our previous study [ 20 ].…”

Section: Discussionsupporting

confidence: 83%

“…Recently, Tattoli et al found that S. Typhimurium escaped from autophagy by an unknown mechanism [ 19 ]. Our previous study demonstrated that the Salmonella virulence plasmid harboring spv genes could enhance intracellular bacterial growth by suppressing autophagy of host cells [ 20 ]. However, the exact mechanisms were not yet elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Proteins were separated by 12% SDS-PAGE, followed by transfer onto a nitrocellulose membrane (Pall Corporation). The following membrane blocking, antibody incubations and chemiluminescence (ECL, Biological Industries) detection were performed according to the reference [ 20 ]. The amount of correlated proteins was analyzed by Image J launcher broken symmetry software program.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous studies had shown that Salmonella virulent plasmid harboring spv genes could enhance bacterial cytotoxicity by inhibiting autophagy of host cells [ 20 ]. It was known that SpvB played an important role in Salmonella pathogenesis by depolymerizing of host cell actin, and polymerization of actin cytoskeleton participates in the early events of autophagosome formation [ 8 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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A novel contribution ofspvBto pathogenesis ofSalmonellaTyphimurium by inhibiting autophagy in host cells

et al. 2016

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Salmonella plasmid virulence genes (spv) are highly conserved in strains of clinically important Salmonella serovars. It is essential for Salmonella plasmid-correlated virulence, although the exact mechanism remains to be elucidated. Autophagy has been reported to play an important role in host immune responses limiting Salmonella infection. Our previous studies demonstrated that Salmonella conjugative plasmid harboring spv genes could enhance bacterial cytotoxicity by inhibiting autophagy. In the present study, we investigated whether spvB, which is one of the most important constituents of spv ORF could intervene in autophagy pathway. Murine macrophage-like cells J774A.1, human epithelial HeLa cells, and BALB/c mice infected with Salmonella Typhimurium wild type, mutant and complementary strains (carrying or free spvB or complemented only with ADP-ribosyltransferase activity of SpvB) were used in vitro and in vivo assay, respectively. To further explore the molecular mechanisms, both SpvB ectopic eukaryotic expression system and cells deficient in essential autophagy components by siRNA were generated. Results indicated that spvB could suppress autophagosome formation through its function in depolymerizing actin, and aggravate inflammatory injury of the host in response to S. Typhimurium infection. Our studies demonstrated virulence of spvB involving in inhibition of autophagic flux for the first time, which could provide novel insights into Salmonella pathogenesis, and have potential application to develop new antibacterial strategies for Salmonellosis.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel contribution ofspvBto pathogenesis ofSalmonellaTyphimurium by inhibiting autophagy in host cells

et al. 2016

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“…pR (ST98) has a molecular weight of 98.6 × 10 6 Da (about 159 kb), and was first identified and reported by Huang et al (2005). Subsequent studies in their laboratory have shown that mutant Salmonella, harboring a pR (ST98) plasmid deletion, inhibited autophagy in infected macrophages (He et al, 2012;Chu et al, 2014;Wu et al, 2014) and mouse embryonic fibroblasts (Lv et al, 2012), while enhancing Salmonella proliferation within cells and consequently promoting cell death. These results demonstrate that the pR (ST98) plasmid enables Salmonella to escape autophagy.…”

Section: The Escape Mechanism Employed By Salmonella To Evade Host Cementioning

confidence: 99%

Salmonella Interacts With Autophagy to Offense or Defense

Shen

Zhang

et al. 2020

Front. Microbiol.

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Autophagy is an important component of the innate immune system in mammals. Low levels of basic autophagy are sustained in normal cells, to help with the clearance of aging organelles and misfolded proteins, thus maintaining their structural and functional stability. However, when cells are faced with challenges, such as starvation or pathogenic infection, their level of autophagy increases significantly. Salmonella is a facultative intracellular pathogen, which imposes an economic burden on the poultry farming industry and human public health. Previous studies have shown that Salmonella can induce the autophagy of cells following invasion, which to a certain extent helps to protect the cells from bacterial colonization. This review summarizes the latest research in the field of Salmonella-induced autophagy, including: (i) the autophagy induction and escape mechanisms employed by Salmonella during the infection of host cells; (ii) the effect of autophagy on intracellular Salmonella; (iii) the important autophagy adaptors that recognize intracellular Salmonella in host cells; and (iv) the effect of autophagy-modulating drugs on Salmonella infection.

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Salmonella plasmid virulence gene spvB enhances bacterial virulence by inhibiting autophagy in a zebrafish infection model

Wang

Gao

et al. 2016

Fish & Shellfish Immunology

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A Salmonella enterica conjugative plasmid impairs autophagic flux in infected macrophages

Cited by 7 publications

References 34 publications

A novel contribution ofspvBto pathogenesis ofSalmonellaTyphimurium by inhibiting autophagy in host cells

A novel contribution ofspvBto pathogenesis ofSalmonellaTyphimurium by inhibiting autophagy in host cells

Salmonella Interacts With Autophagy to Offense or Defense

Salmonella plasmid virulence gene spvB enhances bacterial virulence by inhibiting autophagy in a zebrafish infection model

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