A Salt-Inducible Mn-Catalase (KatB) Protects Cyanobacterium from Oxidative Stress

Chakravarty, Dhiman; Banerjee, Manas; Bihani, Subhash C.; Ballal, Anand

doi:10.1104/pp.15.01632

Cited by 35 publications

(30 citation statements)

References 47 publications

(60 reference statements)

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“…Unlike the wild-type Anabaena PCC 7120, the NaCl-treated katB mutant was found to be particularly sensitive to H 2 O 2 , and exposure to H 2 O 2 caused increased formation of lipid peroxides, oxidized proteins and total peroxides in the katB mutant 13 . In fact, a considerably higher level of oxidized proteins was found in the culture medium of the mutant at the end of 24 h (Fig.…”

mentioning

confidence: 86%

“…Anabaena cells that were pre-treated with NaCl showed high degree of resistance to H 2 O 2 and could withstand exposure to as high as 3 mM H 2 O 2 13 . Keeping in mind the presence of katA / B genes in Anabaena and catalase being the known ‘classical’ enzyme that protects organisms from H 2 O 2 , catalase activity of the above-mentioned cultures was assessed on zymograms.…”

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confidence: 99%

“…The katB promoter- gfp fusion construct 13 was gainfully employed to determine the various osmotic stimuli that could activate katB expression. All the osmolytes tested, i.e.…”

mentioning

confidence: 99%

“…Desiccation, an extreme form of osmotic stress, also induces synthesis of KatB in Anabaena . Also, katB expression is regulated by the nitrogen status of the medium and there is a distinct lack of katB transcription in heterocysts (cells that fix nitrogen) 13 . Thus mechanism underlying the transcriptional activation of katB is complicated and several environmental cues are integrated in this process.…”

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confidence: 99%

“…The presence of these homologs in Anabaena lends support to the idea that katB too may be regulated by proteins such as histidine kinases.

Figure 3.The katB promoter- gfp fusion construct was transformed into Anabaena PCC 7120 and the katB promoter activity was monitored 13 in the presence of various osmolytes such as NaCl (150 mM), sucrose (300 mM), glycerol (300 mM), manitol (300 mM), sorbitol (300 mM), and PEG (100 mM). Cells were exposed to the above-mentioned osmolytes for 18h.…”

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confidence: 99%

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Cyanobacterial Mn-catalase ‘KatB’: Molecular link between salinity and oxidative stress resistance

Chakravarty

Banerjee

Waghmare

et al. 2016

Communicative & Integrative Biology

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Catalases are ubiquitous enzymes that detoxify H2O2 in virtually all organisms exposed to oxygen. The filamentous, nitrogen-fixing cyanobacterium, Anabaena PCC 7120, shows the presence of 2 genes (katA and katB) that encode Mn-catalases. We have recently shown that pre-treatment of Anabaena with NaCl causes substantial induction of the KatB protein, which consequently leads to increased oxidative stress resistance in that cyanobacterium. Interestingly, when compared to the wild-type, the katB mutant shows decreased growth and impaired photosynthetic activity in the presence of NaCl. Furthermore, the NaCl-treated katB mutant is extremely sensitive to H2O2. In this study, the ultrastructural changes occurring in the katB mutant and the wild-type Anabaena cells are analyzed to understand the cellular basis of the above-mentioned protective phenomena. Other data show that a wide variety of osmolytes induce katB expression in Anabaena, indicating that katB is a genuine osmo-inducible gene. These results have important biotechnological implications for the development of novel cyanobacterial biofertilzers and transgenic plants with improved resistance to salinity.

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confidence: 86%

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confidence: 99%

“…The katB promoter- gfp fusion construct 13 was gainfully employed to determine the various osmotic stimuli that could activate katB expression. All the osmolytes tested, i.e.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…The presence of these homologs in Anabaena lends support to the idea that katB too may be regulated by proteins such as histidine kinases.

mentioning

confidence: 99%

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Cyanobacterial Mn-catalase ‘KatB’: Molecular link between salinity and oxidative stress resistance

Chakravarty

Banerjee

Waghmare

et al. 2016

Communicative & Integrative Biology

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Nickel and arsenite‐induced differential oxidative stress and antioxidant responses in two Anabaena species

2018

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In recent years, release of chemical pollutants has increased due to anthropogenic activities. Heterocystous filamentous cyanobacteria constitute dominant paddy microflora and are excellent biofertilizers augmenting rice productivity. Cyanobacteria are frequently exposed to toxic metals, nickel and arsenic are one of the major toxicants present. We exposed two species of diazotrophic cyanobacteria Anabaena sp. PCC 7120 and Anabaena doliolum, to sub-lethal concentrations (15.0 and 9.0 μM) of Ni and (17.0 and 11 mM) of arsenite (AsIII) and analyzed at different days of treatments (0, 1, 7, and 15 days) for oxidative damage and antioxidative biomarkers. Lipid peroxidation was enhanced (1.5- to 2.5-fold increase in MDA content), indicating damaging effects of Ni and As(III) on membrane. Although Ni and As(III), both induced oxidative stress in both species, Anabaena PCC 7120 experienced less stress than A. doliolum. This could be explained by a higher activity of antioxidant enzymes catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR) in Anabaena PCC 7120 (4.6-, 2.0- and 1.4-fold [Ni ] 3.2-, 2.5-, and 2.08-fold [As]) compared to A. doliolum (4.2-, 2.5-, and 1.3-fold [Ni ] and 3.2-, 3.33-, and 1.8-fold [As]). Moreover, superoxide dismutase registered less inhibition in Anabaena sp. PCC 7120 (1.5 and 1.8) compared to A. doliolum (1.8 and 2.3) under Ni and As(III) stress. In addition to, IBR revealed that As(III) imposes severe impact on both strain, however, A. doliolum suffers most. Therefore, the study demonstrates interspecies variation in survival strategy of two Anabaena species and difference in potential of two different toxicants to produce oxidative stress.

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Cyanobacteria: Role in Agriculture, Environmental Sustainability, Biotechnological Potential and Agroecological Impact

Yadav

Rai

et al. 2017

Plant-Microbe Interactions in Agro-Ecological Perspectives

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A Salt-Inducible Mn-Catalase (KatB) Protects Cyanobacterium from Oxidative Stress

Cited by 35 publications

References 47 publications

Cyanobacterial Mn-catalase ‘KatB’: Molecular link between salinity and oxidative stress resistance

Cyanobacterial Mn-catalase ‘KatB’: Molecular link between salinity and oxidative stress resistance

Nickel and arsenite‐induced differential oxidative stress and antioxidant responses in two Anabaena species

Cyanobacteria: Role in Agriculture, Environmental Sustainability, Biotechnological Potential and Agroecological Impact

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