A sample-to-answer, quantitative real-time PCR system with low-cost, gravity-driven microfluidic cartridge for rapid detection of SARS-CoV-2, influenza A/B, and human papillomavirus 16/18

Abstract

The pandemic of coronavirus disease 2019 (COVID-19), due to the novel coronavirus (SARS-CoV-2), has created an unprecedented threat to global health system, especially in resource-limited areas. This challenge shines a...

A sample-to-answer DNA detection microfluidic system integrating sample pretreatment and smartphone-readable gradient plasmonic photothermal continuous-flow PCR

“…20 Although this CF-PCR has a speed gap compared to some ultra-fast PCR devices that complete amplification within 1 minute, it still has significant practical value because its time consumption is at least 50% shorter than that of most benchtop PCR instruments, which greatly simplifies thermal control. 49…”

Centrifugal microfluidic system for colorimetric sample-to-answer detection of viral pathogens

“…Advances in microfluidic technology have made viral pathogen detection a prospective application in medical diagnostics, 24 food safety inspections, 25 and environmental monitoring. 26 Sample-toanswer workflows have been attempted using several fluid actuation schemes, ranging from paper 26,27 and capillarybased devices 28 to electrowetting-on-dielectric, 29 gravitydriven 30 and pressure-mediated flow, 31 as well as centrifugal systems. 32,33 Among these, centrifugal platforms stand out due to the relative simplicity and ease at which microfluidic operations such as liquid transfer, mixing and metering can be implemented, scaled and standardized using a convenient disk or cartridge format.…”

An integrated microfluidic chip for nucleic acid extraction and continued cdPCR detection of pathogens

“…7,8 PCR-based nucleic acid detection is now considered the gold standard for infectious disease diagnosis. 9 It can rapidly and efficiently amplify the characteristic nucleotide sequences of pathogens and has become an important tool for disease diagnosis. The standard method of nucleic acid detection includes two basic steps: nucleic acid extraction and nucleic acid amplification detection.…”