A sandwich ELISA for porcine alpha-1 acid glycoprotein (pAGP, ORM-1) and further demonstration of its use to evaluate growth potential in newborn pigs

Caperna, Thomas J.; Shannon, Amy E.; Stoll, M. J.; Kahl, Stanislaw; Blomberg, Le Ann; Vallet, J. L.; Ramsay, Timothy

doi:10.1016/j.domaniend.2017.04.001

Cited by 9 publications

(11 citation statements)

References 19 publications

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“…Circulatory AGP predicts differences in growth rate at day 1 of age [2] [3]. This was confirmed in the present study with the control litters in which AGP predicted the lower growth rates of pigs with higher concentrations of plasma AGP.…”

Section: Discussionsupporting

confidence: 87%

“…Plasma samples prepared from the vials were used for analysis of AGP concentration on day 2 post farrowing by ELISA analysis [3]. Piglets were cross-fostered on day 3 at approximately 08:00 and distributed among the foster recipient sows in pairs according to their birth weight, gender and a difference in plasma AGP > 500 µg/mL [3].…”

Section: Methodsmentioning

confidence: 99%

“…Piglets were cross-fostered on day 3 at approximately 08:00 and distributed among the foster recipient sows in pairs according to their birth weight, gender and a difference in plasma AGP > 500 µg/mL [3]. In no case did a piglet suckle its own mother after cross-fostering.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Recent research has demonstrated that neonatal serum or plasma AGP can be used at birth to identify pigs that will grow poorly and can also be used to predict overall litter performance [2] [3]. The circulating concentration of AGP in the newborn is negatively correlated with postnatal growth rate up to 8 weeks of age [3]. Neonatal pigs of an average litter birth weight but with the highest circulating concentrations of AGP will grow more slowly than littermates of similar birth weight [2] and have a different body composition after weaning [4].…”

Section: Introductionmentioning

confidence: 99%

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Use of Cross-Fostering to Enhance Growth of Pigs That Are Predicted to Grow Poorly Based on Plasma α-1 Acid Glycoprotein Concentration

Ramsay¹,

Stoll²,

Schreier³

et al. 2018

OJAS

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Porcine α-1 acid glycoprotein (AGP) in newborn pigs can be used to predict growth rate through weaning and is a marker for growth impairment. This study examined whether nutritional support can improve the growth rate of piglets identified as having poor growth potential. Cross-fostering (CF) and CF plus a milk supplement (CF + MS) were used to attempt to improve the growth performance of pigs. Blood was collected at d1 post-parturition for measurement of plasma AGP for all pigs in 28 litters contributing to the experiment. Piglets with the highest plasma AGP level were weight and sex matched to a littermate with a low plasma AGP concentration and four pairs of these weight and sex matched pigs were grouped into four foster litters per treatment (control, CF, CF + MS). The control group was assembled by pairing littermates remaining in donor litters. Pigs stayed on treatment until weaning at 21 days of age. At 35 days of age, dual energy X-ray absorptiometry (DXA) was performed on CF and CF + MS pigs to evaluate carcass composition. Control pairs differed in weaning weight, with pigs with higher plasma AGP at 1 day of age having smaller weaning weights than their littermates of similar birth weight (P < 0.05). However, CF eliminated the difference in weaning weight between the slow growing pigs and their birth weight matched littermates. CF + MS produced a similar effect as CF (P > 0.05). At 35 days of age, body weights were still similar between CF littermates and between CF + MS littermates (P > 0.05). DXA analysis demonstrated that body composition was similar between CF or CF + MS treated pigs and their litter-

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Section: Discussionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Use of Cross-Fostering to Enhance Growth of Pigs That Are Predicted to Grow Poorly Based on Plasma α-1 Acid Glycoprotein Concentration

Ramsay¹,

Stoll²,

Schreier³

et al. 2018

OJAS

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Proteomic methods for the study of porcine acute phase proteins – anything new to detect?

Miller

Gianazza

2023

Vet Res Commun

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Acute phase proteins (APPs) reflect the health status of individuals and are important tools in diagnostics, as their altered levels are a sign of disturbed homeostasis. While, in most cases, quantitation of known serum APPs is routinely performed by immunoassays, proteomics is helpful in discovery of new biomarker candidates, especially in samples other than body fluids. Besides putting APP regulation into an overall context of differentially abundant proteins, this approach can detect further details or outright new features in protein structure or specific modifications, and help understand better their function. Thus, it can show up ways to make present diagnostic assays more sensitive and/or specific, or correlate regulations of disease-specific proteins. The APP repertoire is dependent on the species. The pig is both, an important farm animal and a model animal for human diseases, due to similarities in physiology. Besides reviewing existing literature, yet unpublished examples for two-dimensional electrophoresis in connection with pig APPs highlight some of the benefits of proteomics. Of further help would be the emerging targeted proteomics, offering the possibility to determine particular isoforms or proteoforms, without the need of specific antibodies, but this method is presently scarcely used in veterinary medicine.

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Peripheral histamine and neonatal growth performance in swine

Ramsay

Kahl

Long

et al. 2020

Domestic Animal Endocrinology

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A sandwich ELISA for porcine alpha-1 acid glycoprotein (pAGP, ORM-1) and further demonstration of its use to evaluate growth potential in newborn pigs

Cited by 9 publications

References 19 publications

Use of Cross-Fostering to Enhance Growth of Pigs That Are Predicted to Grow Poorly Based on Plasma α-1 Acid Glycoprotein Concentration

Use of Cross-Fostering to Enhance Growth of Pigs That Are Predicted to Grow Poorly Based on Plasma α-1 Acid Glycoprotein Concentration

Proteomic methods for the study of porcine acute phase proteins – anything new to detect?

Peripheral histamine and neonatal growth performance in swine

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