A sandwich ELISA for the detection of fish and fish products

Development and Characterization of Monoclonal Antibodies for the Detection of Fish Protein

“…The pAb previously used in the sELISA apparently recognized the same major 36 kDa protein in all 63 cooked fish samples tested [20]. This supports our contention that this protein is not only highly antigenic, but also ubiquitously present in all common food fish species with fish-specific epitopes, making it an ideal biomarker for the immunodetection of fish.…”

“…The pAb reagent used in the previously reported sELISA, which successfully detected all 63 fish species tested, also recognized a major 36 kDa protein band in all the cooked fish samples [20]. The 36 kDa protein recognized by mAb 8F5 and pAb could be the same antigenic protein because their immunogens were similarly prepared in our laboratory.…”

“…In an earlier study, we developed a sandwich enzyme-linked immunosorbent assay (sELISA) using pAbs raised against a mixture of crude protein extracts of cooked fish muscle from ten species (Atlantic salmon, yellowfin tuna, swordfish, black grouper, tilapia, red snapper, amberjack, basa, catfish, and perch) [20]. After cleaning by immunoabsorption, the pAbs recognized a major 36 kDa protein band in the sample protein extract.…”

Commercial fish ELISA kits have a limited capacity to detect different fish species and their products

“…9,11,36,37 This region contributes 89% of the global aquaculture and over 50% of the world's wild fish catch (Asia-Pacific Fishery Commission, FAO 2019). Some inhouse ELISA assays have been developed for different fish species, [38][39][40] however, they are not commercially available. It is to note that none of these ELISA approaches are suitable for fish species authentication.…”

Development of a real-time PCR system for the detection of the potential allergen fish in food

“…For detection of fish allergens in food, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) technology are used most widely [7][8][9][10][11]. The majority of ELISA systems are based on the detection of the major fish allergenic protein, parvalbumin [8].…”