A sandwich-type electrochemical immunosensor using Ag@CeO2-Au as a lable for sensitive detection of carcinoembryonic antigen

Chen, Siyu; Yang, Yuxiao; Li, Wenjun; Song, Yiju; Shi, Lei

doi:10.1016/j.microc.2020.105415

Cited by 19 publications

(10 citation statements)

References 40 publications

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“…Carcinoma embryonic antigen (CEA) is a cell adhesive acidic glycoprotein with properties similar to the human embryonic cell. Normally, the level of CEA is around 5 µg/mL in serum, but in the blood the level is very low (<5 ng/mL) [ 195 , 196 , 197 , 198 ]. Meanwhile, serum CEA has been found to elevate up to 20 µg/mL in people with lung cancer [ 197 ].…”

Section: Mnp-based Ecbs For Biomolecule Detectionmentioning

confidence: 99%

“…In several other types of carcinomas, such as breast cancer, ovarian cancer, pancreatic cancer, and gastrointestinal cancer, CEA often shows elevation in serum level, which indicates its potentiality as being a tumor marker for clinical cancer diagnosis [ 195 , 196 , 197 ]. Therefore, quantitative measurements of CEA in biological fluids such as blood and serum are critical for locating, and understanding the prognosis, staging, and recurrence of multiple cancers [ 197 , 198 , 199 , 200 , 201 ]. For electrochemical immunosensor/aptasensor assays, MNP-based probes which have strong biocompatibility and electrical conductivity are of great interest [ 201 , 202 , 203 , 204 , 205 ].…”

Section: Mnp-based Ecbs For Biomolecule Detectionmentioning

confidence: 99%

“…For electrochemical immunosensor/aptasensor assays, MNP-based probes which have strong biocompatibility and electrical conductivity are of great interest [ 201 , 202 , 203 , 204 , 205 ]. MNPs have superior efficiency as tags or labels for amplifying biomolecular interactions and as the enhancers of electrochemical signals [ 198 , 199 , 200 , 201 , 202 , 203 ]. Depending upon the complexity and necessities, mono/bi/tri-metallic composites are utilized for constructing different types of ECBs with label-free or labeled, sandwich or non-sandwich strategies for CEA immunosensor/aptasensor [ 195 , 196 , 199 , 207 , 210 ].…”

Section: Mnp-based Ecbs For Biomolecule Detectionmentioning

confidence: 99%

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Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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With the rise in public health awareness, research on point-of-care testing (POCT) has significantly advanced. Electrochemical biosensors (ECBs) are one of the most promising candidates for the future of POCT due to their quick and accurate response, ease of operation, and cost effectiveness. This review focuses on the use of metal nanoparticles (MNPs) for fabricating ECBs that has a potential to be used for POCT. The field has expanded remarkably from its initial enzymatic and immunosensor-based setups. This review provides a concise categorization of the ECBs to allow for a better understanding of the development process. The influence of structural aspects of MNPs in biocompatibility and effective sensor design has been explored. The advances in MNP-based ECBs for the detection of some of the most prominent cancer biomarkers (carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), Herceptin-2 (HER2), etc.) and small biomolecules (glucose, dopamine, hydrogen peroxide, etc.) have been discussed in detail. Additionally, the novel coronavirus (2019-nCoV) ECBs have been briefly discussed. Beyond that, the limitations and challenges that ECBs face in clinical applications are examined and possible pathways for overcoming these limitations are discussed.

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Section: Mnp-based Ecbs For Biomolecule Detectionmentioning

confidence: 99%

Section: Mnp-based Ecbs For Biomolecule Detectionmentioning

confidence: 99%

Section: Mnp-based Ecbs For Biomolecule Detectionmentioning

confidence: 99%

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Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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“…Acta. 88, 135-140. doi: 10.1016/j.electacta.2012.10.081 Xu, Q., Jia, H., Duan, X., Lu, L., Tian, Q., Chen, S., et al (2020). Labelfree electrochemical immunosensor for the detection of prostate specific antigen based three-dimensional Au nanoparticles/MoS 2graphene aerogels composite.…”

Section: Data Availability Statementmentioning

confidence: 99%

“…Although the immunosensors show high performance in the detection of target analytes, some devices present limits of detection (LOD) comparable to those of label-free configuration, which has less complexity. Chen et al ( 2020 ) reported that a sandwich-type sensor with a dual-signal amplification element mechanism used in the detection of carcinoembryonic antigen (CEA) obtained an LOD of 0.032 pg ml −1 . Additionally, signal amplification using high-surface area graphene (GP) nanocomposites presented an LOD value of 100 pg ml −1 (Chen et al, 2013 ), and Ag/Au nanoparticles (NPs)/GP provided an LOD value of 8 pg ml −1 (Huang et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing

Chanarsa

Jakmunee

2021

Front. Chem.

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A bi-functional material based on silver nanoparticles (AgNPs)-reduced graphene oxide (rGO) composite for both electrode modification and signal generation is successfully synthesized for use in the construction of a label-free electrochemical immunosensor. An AgNPs/rGO nanocomposite is prepared by a one-pot wet chemical process. The AgNPs/rGO composite dispersion is simply cast on a screen-printed carbon electrode (SPCE) to fabricate the electrochemical immunosensor. It possesses a sufficient conductivity/electroreactivity and improves the electrode reactivity of SPCE. Moreover, the material can generate an analytical response due to the formation of immunocomplexes for detection of human immunoglobulin G (IgG), a model biomarker. Based on electrochemical stripping of AgNPs, the material reveals signal amplification without external redox molecules/probes. Under optimized conditions, the square wave voltammetric peak current is responded to the logarithm of IgG concentration in two wide linear ranges from 1 to 50 pg.ml−1 and 0.05 to 50 ng.ml−1, and the limit of detection (LOD) is estimated to be 0.86 pg.ml−1. The proposed immunosensor displays satisfactory sensitivity and selectivity. Importantly, detection of IgG in human serum using the immunosensor shows satisfactory accuracy, suggesting that the immunosensor possesses a huge potential for further development in clinical diagnosis.

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Coronary Au@Bi Nanospheres for Highly Impedance‐Sensitive Immunosensing toward CEA

Wang

Ouyang

Liu

et al. 2023

Advanced Sensor Research

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Novel coronary Au@Bi nanospheres (NSs) are simply synthesized by sparkling gold nanoparticles (Au NPs) on the surface of bismuth (Bi) NSs and successfully used as highly impedance‐sensitive immunosensing platform to develop a new label‐free electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA). Firstly, Bi NSs are prepared by one‐pot hydrothermal method, showing uniform sphere morphology with a dense oxide film formed outside Bi NSs due to the easy oxidation of Bi in the air. The oxide film significantly hinders the transmission of electrons and effectively improves the sensitivity of the proposed electrochemical impedance immunosensor. The surface functionalization of Bi NSs with Au NPs enhances the biocompatibility, stability, as well as active surface area of the produced Au@Bi NSs, preferable for the efficient loading of antibody molecules. The electrochemical impedance spectroscopy (EIS) is selected as the optical technique to measure CEA with the constructed immunosensor based on Au@Bi NSs. Because of the excellent impedance‐responsive performance, this new immunosensor displays highly sensitive detection toward CEA in a linear range of 50 fg mL−1–100 ng mL−1 with an extremely low detection limit of 9.83 fg mL−1 and could be potentially used in early clinical detection of cancer.

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A sandwich-type electrochemical immunosensor using Ag@CeO2-Au as a lable for sensitive detection of carcinoembryonic antigen

Cited by 19 publications

References 40 publications

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing

Coronary Au@Bi Nanospheres for Highly Impedance‐Sensitive Immunosensing toward CEA

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