A Satellite RNA of Groundnut Rosette Virus that Is Largely Responsible for Symptoms of Groundnut Rosette Disease

Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts

“…4, lane C). The dsRNA pattern obtained by immunoblotting was identical to the pattern described in (10), and two of the major dsRNA species were tentatively identified as dsRNA-1 and dsRNA-3 (Fig. 4).…”

“…All dsRNAs represent replicative forms and replication intermediates of GRV genomic RNA and of its satellite RNA. On the basis of their relative abundance and electrophoretic mobility two of the major dsRNAs were tentatively identified as dsRNA-1 (RF of genomic RNA) and dsRNA-3 (highly abundant ds-satellite RNA) according to the nomenclature given in (10). Reovirus dsRNAs were used as MW markers (M; MW range 3.8 kbp to 1.2 kbp).…”

“…In purified dsRNA preparations from GRV infected plants showing chlorotic or green types of rosette symptoms three prominent (dsRNA 1-3) and several minor dsRNA species were detected. Two of the major dsRNAs, dsRNA-1 (4.6 kbp) and dsRNA-2 (1.3 kbp) both hybridize to the genomic ssRNA and probably represent its replicative forms (10). The highly abundant dsRNA-3 (900 bp) seems to be a satellite RNA which is probably responsible for the symptoms of groundnut rosette (10,11).…”

“…Two of the major dsRNAs, dsRNA-1 (4.6 kbp) and dsRNA-2 (1.3 kbp) both hybridize to the genomic ssRNA and probably represent its replicative forms (10). The highly abundant dsRNA-3 (900 bp) seems to be a satellite RNA which is probably responsible for the symptoms of groundnut rosette (10,11). By using dsRNA specific MAB we detected the same dsRNA species in aqueous leaf extracts as those described in the literature (10) and determined the thermal denaturation profile of dsRNA-3.…”

Groundnut Rossette: A Virus Disease Affecting Groundnut Production in Sub-Saharan Africa

“…GRAV, GRV, and sat RNA are intricately dependent on each other, and all three play a crucial role in the biology and perpetuation of the disease. The sat RNA was shown to be largely responsible for disease symptoms (48). Variants of sat RNA cause different forms of the disease (46,48), whereas GRAV or GRV alone cause asymptomatic infections.…”