Abstract:Human mesenchymal stem cells (hMSCs) are effective in treating disorders resulting from an inflammatory or heightened immune response. The hMSCs derived from induced pluripotent stem cells (ihMSCs) share the characteristics of tissue derived hMSCs but lack challenges associated with limited tissue sources and donor variation. To meet the expected future demand for ihMSCs, there is a need to develop scalable methods for their production at clinical yields while retaining immunomodulatory efficacy. Herein, we de… Show more
“…Obviously, CM/GN facilitated cell proliferation on 4th and 7th day and the promoting effect hoisted with GelNB content increasing. It was explained that gelatin, the raw material for GelNB synthesis, was a species of protein and it would release nutrient substance for cell growth during degradation, leading to the acceleration of BMSCs proliferation ( Rogers et al., 2021 ). Figure 5 Cytotoxicity of CM/GN hydrogels (A) Live/Dead assay of BMSCs cultured with extract of CM/GN hydrogels at day 1 and day 4: images (left, scale bar: 200 μm) and percentage of viability for each group (right).…”
“…Obviously, CM/GN facilitated cell proliferation on 4th and 7th day and the promoting effect hoisted with GelNB content increasing. It was explained that gelatin, the raw material for GelNB synthesis, was a species of protein and it would release nutrient substance for cell growth during degradation, leading to the acceleration of BMSCs proliferation ( Rogers et al., 2021 ). Figure 5 Cytotoxicity of CM/GN hydrogels (A) Live/Dead assay of BMSCs cultured with extract of CM/GN hydrogels at day 1 and day 4: images (left, scale bar: 200 μm) and percentage of viability for each group (right).…”
“…As a result, there has been a great interest in generating MSCs from alternative sources. One such novel alternative method is to generate MSCs (iMSCs) from iPSCs[ 3 , 4 ]. iPSCs are a well-characterized source of pluripotent cells.…”
Section: To the Editormentioning
confidence: 99%
“…iMSCs are a next generation alternative source of stem cells with potential therapeutic applications in regenerative medicine[ 3 , 4 , 9 ]. iPSC-derived iMSCs could significantly elevate the therapeutic values for clinical applications.…”
Mesenchymal stem cells (MSCs) have the potential for use in cell-based regenerative therapies. Currently, hundreds of clinical trials are using MSCs for the treatment of various diseases. However, MSCs are low in number in adult tissues; they show heterogeneity depending upon the cell source and exhibit limited proliferative potential and early senescence in
in vitro
cultures. These factors negatively impact the regenerative potential of MSCs and therefore restrict their use for clinical applications. As a result, novel methods to generate induced MSCs (iMSCs) from induced pluripotent stem cells have been explored. The development and optimization of protocols for generation of iMSCs from induced pluripotent stem cells is necessary to evaluate their regenerative potential
in vivo
and
in vitro
. In addition, it is important to compare iMSCs with primary MSCs (isolated from adult tissues) in terms of their safety and efficacy. Careful investigation of the properties of iMSCs
in vitro
and their long term behavior in animals is important for their translation from bench to bedside.
“…The culture, scale up of manufacturing and processing of adherent cells requires innovative solutions to enable translation of emerging cell-based technologies in the fields of regenerative medicine and cultivated meat [1][2]. Although a range of platforms have been designed and proposed to answer these challenges, from hollow fibre reactors to plastic and hydrogel microcarriers for stirred tank reactors [3][4][5][6], these remain associated with complex cell processing steps, as well as high costs and risks of contamination with microplastics.…”
Bioemulsions are attractive platforms for the expansion of adherent cells in bioreactors. Their design relies on the self-assembly of protein nanosheets at liquid-liquid interfaces, displaying strong interfacial mechanical properties and promoting integrin-mediated cell adhesion. However, most systems developed to date have focused on fluorinated oils, which are unlikely to be accepted for direct implantation of resulting cell products for regenerative medicine, and protein nanosheets self-assembly at other interfaces has not been investigated. In this report, the composition of aliphatic pro-surfactants palmitoyl chloride and sebacoyl chloride, on the assembly kinetics of poly(L-lysine) at silicone oil interfaces and characterisation of ultimate interfacial shear mechanics and viscoelasticity is presented. The impact of the resulting nanosheets on the adhesion of mesenchymal stem cells (MSCs) is investigated via immunostaining and fluorescence microscopy, demonstrating the engagement of the classic focal adhesion-actin cytoskeleton machinery. The ability of MSCs to proliferate at the corresponding interfaces is quantified. In addition, expansion of MSCs at other non-fluorinated oil interfaces, based on mineral and plant-based oils is investigated. Finally, the proof-of-concept of such non-fluorinated oil systems for the formulation of bioemulsions supporting stem cell adhesion and expansion is demonstrated.
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