A scanning electron microscopic evaluation of in vitro dentinal tubules penetration by selected anaerobic bacteria

Siqueira, José F.; Uzeda, Milton de; Fonseca, M. E. F.

doi:10.1016/s0099-2399(96)80265-2

Cited by 101 publications

(78 citation statements)

References 14 publications

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“…On the other hand, it has been reported that Porphyromonas endodontalis BN11a-f and P. gingivalis ATCC 33277 in monoculture were able to invade bovine dentinal tubules, although very few tubules were infected (40). While the number, distribution, and size of bovine dentinal tubules are similar to those in human dentin, Siqueira et al (40) removed the cementum from the dentin blocks in their experiments, and this artificially enhances bacterial penetration. Thus, the reported penetration of very few tubules may have occurred by nonspecific incorporation and not through cell division and growth along the tubule length.…”

Section: Discussionmentioning

confidence: 99%

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

et al. 2000

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Cell wall-anchored polypeptides of the antigen I/II family are produced by many species of oral streptococci. These proteins mediate adhesion of streptococci to salivary glycoproteins and to other oral microorganisms and promote binding of cells to collagen type I and invasion of dentinal tubules. Since infections of the root canal system have a mixed anaerobic bacterial etiology, we investigated the hypothesis that coadhesion of anaerobic bacteria with streptococci may facilitate invasive endodontic disease. Porphyromonas gingivalis ATCC 33277 cells were able to invade dentinal tubules when cocultured with Streptococcus gordonii DL1 (Challis) but not when cocultured with Streptococcus mutans NG8. An isogenic noninvasive mutant of S. gordonii, with production of SspA and SspB (antigen I/II family) polypeptides abrogated, was deficient in binding to collagen and had a 40% reduced ability to support adhesion of P. gingivalis. Heterologous expression of the S. mutans SpaP (antigen I/II) protein in this mutant restored collagen binding and tubule invasion but not adhesion to P. gingivalis or the ability to promote P. gingivalis coinvasion of dentin. An isogenic afimbrial mutant of P. gingivalis had 50% reduced binding to S. gordonii cells but was unaffected in the ability to coinvade dentinal tubules with S. gordonii wild-type cells. Expression of the S. gordonii SspA or SspB polypeptide on the surface of Lactococcus lactis cells endowed these bacteria with the abilities to bind P. gingivalis, penetrate dentinal tubules, and promote P. gingivalis coinvasion of dentin. The results demonstrate that collagen-binding and P. gingivalis-binding properties of antigen I/II polypeptides are discrete functions. Specificity of antigen I/II polypeptide recognition accounts for the ability of P. gingivalis to coinvade dentinal tubules with S. gordonii but not with S. mutans. This provides evidence that the specificity of interbacterial coadhesion may influence directly the etiology of pulpal and periapical diseases.

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Section: Discussionmentioning

confidence: 99%

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

et al. 2000

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“…Migration of dormant bacteria: Bacteria residing in anatomical areas of the root canal such as isthmuses, apical ramifications, irregularities, deltas and dentinal tubules may remain unaffected by disinfection procedures [Siqueira et al 1996] and survive by deriving nutrients from tissue remnants, although some might be killed or prevented from gaining access to the periradicular tissues after the canals are obturated. However, if the obturation seal is inadequate (under extended), it may allow seepage of tissue fluids providing a substrate for bacterial growth, which, then gain access to the periradicular area and cause periapical inflammation [Siqueira 2001].…”

Section: )mentioning

confidence: 99%

Endodontic flare ups: A proposal for etiopathogenesis and psychological management

Kaur

2015

IJH

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<p>Flare ups are undesirable yet unavoidable, true endodontic emergencies, presenting with severe pain and/or swelling of the facial soft tissues and the oral mucosa in the area of endodontically treated tooth, in association with strong expression of clinical symptoms (tooth pain when biting, chewing or by itself), occuring within a few hours or a few days of instrumentation. Prevention and management of such situations necessitates standardized execution of all the steps comprising root canal therapy alongwith comprehensive knowledge of its possible etiological factors, pathogenesis, pharmacological and non-pharmacological treatment methods. This article presents a pro-posal for etiopathogenesis and psychological management besides accounting a brief description of its definition, epidemiology, treatment steps and prevention.</p>

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“…1 Based on the available data, the studies mostly suggest the polymicrobial infection related with root canals as the reason for failure of endodontic treatment. 2 Ability to survive in the poor environmental conditions for long term (12 months) 3 and penetration capacity into dentinal tubules, isthmuses and lateral canals 4 might be the most prominent features that enable the microorganisms to provide infection persistence. Thus, researchers attempt to improve some alternative methods which might provide the total remove of remaining filling materials or the achievement to possible access points within the root canals in spite of the filling materials.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Ethylenediamine Tetraacetic Acid and Calcium Hydroxide in the Removal of Root Canal Filling During Retreatment

Güven¹

2017

Cumhuriyet Dental Journal

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A B S T R A C TObjective: To compare the effect of Ethylenediamine tetraacetic acid (EDTA) and calcium hydroxide [Ca(OH)2] in the removal of root canal filling material during retreatment procedure.Methodology: Eighty extracted, single-rooted human teeth were used. Root canals were shaped with a crown-down technique using Ni-Ti rotary instruments (ProTaper, Dentsply Maillefer, Switzerland) and filled with a cold lateral condensation method. AH Plus was used as a sealer. After filling, the teeth were divided into 4 groups and root fillings were removed by ProTaper Universal rotary retreatment system. In each group different irrigation and disinfection protocols were followed; Group I. Sodium hypochlorite (5.25%) + EDTA (17%-1 min.) Group II. Sodium hypochlorite (5.25%) Group III. Sodium hypochlorite (5.25%) + Ca (OH)2 Group IV. Sodium hypochlorite (5.25%) + Ca(OH)2 + EDTA (17%-1 min.). Irrigation with 17% EDTA was performed only before D3-Retreatment instrumentation in all groups. AutoCAD system was used to detect the amount of residual root canal filling material. The results were statistically analyzed by SPSS (Statistical Package for Social Sciences) for Windows 15.0, one way ANOVA and Tukey's HSD tests. The significance was evaluated at the level of p<0.05. Results:The amount of the remnant filling material in the root canals retreated in Group I was less than the amount of the material in Group II (p<0.01) and Group III (p<0.05). There was no significant difference observed between the other groups in terms of the quantity of the remnant root canal filling materials (p>0.05).Conclusions: Irrigating the canals with EDTA enhanced the removal of root canal filling during retreatment. However, disinfecting the canals with Ca(OH)2 had no effect in the removal of filling material.

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A scanning electron microscopic evaluation of in vitro dentinal tubules penetration by selected anaerobic bacteria

Cited by 101 publications

References 14 publications

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

Endodontic flare ups: A proposal for etiopathogenesis and psychological management

Efficacy of Ethylenediamine Tetraacetic Acid and Calcium Hydroxide in the Removal of Root Canal Filling During Retreatment

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