A Scanning Electron Microscopic Study of Surface Changes during Development of Artificial Caries

Holmen, L.; Thylstrup, A.; Featherstone, J.D.B.; Fredebo, L.; Shariati, Mohammad

doi:10.1159/000260825

Cited by 32 publications

(26 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also possible that enamel abrasion played a role in the reduction of fluorescence loss. It has been reported that abrasion or polishing of demineralized surfaces can result in the exposure of more tightly packed crystals [Holmen et al, 1985] explaining the change in ⌬ F. However, this cannot be the only mechanism responsible as the fluoride-containing groups clearly exhibited statistically superior remineralization.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Autofluorescence to Detect the Remineralization Capabilities of Sodium Fluoride, Monofluorophosphate and Non-Fluoride Dentifrices

Feng

Yin

et al. 2007

Caries Res

View full text Add to dashboard Cite

The purpose of the study was to determine if longitudinal measurements of enamel autofluorescence (quantitative light-induced fluorescence, QLF) could detect differences in remineralization of early enamel caries on buccal surfaces of anterior teeth following supervised daily brushing with either sodium fluoride (NaF; 1,450 ppm F), sodium monofluorophosphate (MFP; 1,450 ppm F) dentifrices or a herbal, non-fluoride placebo dentifrice. The study was a pragmatic cluster-randomized controlled trial with schools as the unit of randomization. Twenty-one schools in Chengdu, China, comprised the clusters; 296 children with at least 1 visible white-spot lesion were examined using QLF at baseline and after 3 and 6 months. Each of the 21 clusters was randomly assigned 1 of the 3 dentifrices, and the childrenbrushed under supervision once per day for 2 min. The primary outcome measure was ΔQ (product of fluorescence loss and area) at a 5% threshold after 6 months of product use. A multi-level model was fitted to the data at the site level, taking into account the hierarchical structure with baseline ΔQ, age and sex as covariates. After 3 months there was a significant difference between the MFP group and placebo (p = 0.02) and after 6 months between the NaF group (p = 0.002), MFP group (p < 0.001) and the placebo. QLF methodology could detect, within 3- and 6-month periods of supervised brushing, a difference in remineralization between fluoride-containing and non-fluoride-containing dentifrices. Typically lesions in all 3 treatment groups demonstrated improvement. Groups receiving fluoride experienced a more rapid and more substantial remineralization than those in the placebo group.

show abstract

Section: Discussionmentioning

confidence: 99%

Assessment of Autofluorescence to Detect the Remineralization Capabilities of Sodium Fluoride, Monofluorophosphate and Non-Fluoride Dentifrices

Feng

Yin

et al. 2007

Caries Res

View full text Add to dashboard Cite

show abstract

“…The SEM photographs show qualitatively an increase in the number of features, which will increase the surface roughness. Holmen et al [1985] suggested from SEM studies of the development of artificial caries that acid exposure opened up the intercrystalline spaces, thus it is to be expected that there would be an increase in surface roughness.…”

Section: Main Experimentsmentioning

confidence: 99%

Optical Profilometric Study of Changes in Surface Roughness of Enamel during in vitro Demineralization

Zhang

Anderson²,

Dowker³

et al. 2000

Caries Res

View full text Add to dashboard Cite

The application of non–contact optical profilometry for non–destructive study of changes in the surface roughness of natural enamel surfaces during in vitro demineralization was investigated. Repeated measurement of profiles of the same enamel surfaces after successive demineralization episodes was achieved by a kinematic specimen mount that could be removed, then accurately repositioned. Surface roughness parameters and reflectivity of natural enamel surfaces exposed to either a demineralizing solution (test) or deionized water (control) for up to 6 days were measured at 6– to 24–hour intervals. The results prior to demineralization showed that surface roughness varies with position on the enamel surface. During demineralization there was an approximately linear increase in surface roughness for the first 70 h followed by somewhat erratic behaviour, and a saturating exponential with time increase in reflectivity for the first 70 h which subsequently remained constant. Changes in enamel surface during in vitro demineralization were also observed using scanning electron microscopy.

show abstract

“…12 Based on chemical analysis and histopathological observations, the initial stage of caries development is characterized by the opening of the intercrystalline spaces without the destruction of the surface and subsequent creation of microchannels. [13][14][15] These microchannels are found to be about 0.5 to 1.5 μm in width in artificial lesions 5 and range from 0.2 to 1.0 μm in width in early natural enamel lesions. 16 When white spot lesions (the early stage of demineralized enamel) are hydrated/wet, microchannels in lesions are filled with fluid/water.…”

Section: Introductionmentioning

confidence: 99%

Pilot clinical study to assess caries lesion activity using quantitative light-induced fluorescence during dehydration

et al. 2017

View full text Add to dashboard Cite

"Pilot clinical study to assess caries lesion activity using quantitative light-induced fluorescence during dehydration," J. Biomed. Opt. Abstract. This study aimed to evaluate the ability of quantitative light-induced fluorescence (QLF) to assess caries lesion activity using visual examination (VE) as the gold standard. Twenty-four visible white spot lesions on buccal surfaces were examined from 23 children, ages 9 to 14 years. At baseline, the surface was hydrated with water, and thereafter, it was dehydrated with continuous compressed air during image acquisition. QLF images were acquired at 0 (baseline), 5, and 15 s. QLF variables [QLF V : fluorescence loss (ΔF), lesion size (S), ΔQ: ΔF × S] was recorded. Changes-in-QLF V per second (ΔQLF V ) were determined: ΔQLF V ¼ ðQLF VN − QLF VBaseline Þ∕N, where N indicates dehydration time. One experienced dentist conducted VE independently using a dental unit's light, compressed air, and explorer. QLF V and ΔQLF V of the active group (n ¼ 11) were compared with those of the inactive group (n ¼ 13) using two-sample t -tests. As the surface was dehydrated, S and ΔQ values of the active group increased, whereas QLF V of the inactive group showed only a small change. ΔQLF V of the active group were larger than those of the inactive group; however, the difference did not reach statistical significance (p > 0.11). Within the limitations of this study, QLF data indicated increments for lesions designated as active and minimal change for lesions defined as inactive.

show abstract

A Scanning Electron Microscopic Study of Surface Changes during Development of Artificial Caries

Cited by 32 publications

References 11 publications

Assessment of Autofluorescence to Detect the Remineralization Capabilities of Sodium Fluoride, Monofluorophosphate and Non-Fluoride Dentifrices

Assessment of Autofluorescence to Detect the Remineralization Capabilities of Sodium Fluoride, Monofluorophosphate and Non-Fluoride Dentifrices

Optical Profilometric Study of Changes in Surface Roughness of Enamel during in vitro Demineralization

Pilot clinical study to assess caries lesion activity using quantitative light-induced fluorescence during dehydration

Contact Info

Product

Resources

About