A scoring system for histopathologic and immunohistochemical evaluations of uterine leiomyosarcomas

Yoshida, Chikara; Ichimura, Tsuyoshi; Kawamura, Naoki; Nakano, Akemi; Kasai, Mari; Sumi, Toshiyuki; Ishiko, Osamu

doi:10.3892/or_00000493

Cited by 12 publications

(9 citation statements)

References 11 publications

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“…In the IHC evaluation of Ki-67 using surgical specimens, all LMS specimens showed an LI of ≥15%. Therefore, we classified the Ki-67 LI as 5% and 15% [8]. In the present study, the expression of Ki-67 was high in LMS, but there was no difference between AL and LMS.…”

Section: Discussionmentioning

confidence: 63%

“…The sections were counterstained with Mayer's hematoxylin. The specificity of the IHC reactions was checked by omitting the primary antibody [8]. …”

Section: Methodsmentioning

confidence: 99%

“…We have attempted to improve the preoperative diagnosis using immunohistochemistry (IHC). We previously reported that the expression of Ki-67 in LMS was higher than that in UL using biopsy specimens [8]. …”

Section: Introductionmentioning

confidence: 99%

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Preoperative Diagnosis of Usual Leiomyoma, Atypical Leiomyoma, and Leiomyosarcoma

Matsuda

Ichimura

Kasai

et al. 2014

Sarcoma

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Uterine smooth muscle tumors (SMTs) are common pelvic tumors in women, and most of them are diagnosed as usual leiomyoma (UL). Exclusion of malignant disease is important in the management of SMTs. However, differentiation of SMTs remains difficult. In this study, we aimed to improve the preoperative diagnosis of SMTs. We examined 21 ULs, 7 atypical leiomyomas (ALs), and 6 leiomyosarcomas (LMSs), all of which were diagnosed by uterine tumor biopsy. Immunohistochemical findings (low-molecular-mass polypeptide 2 (LMP2) and Ki-67) and clinical features (serum lactate dehydrogenase level and menopause) were evaluated. Statistically significant differences in the expression of LMP2 and Ki-67 were observed between UL and AL and between UL and LMS. The combined LMP2 and Ki-67 score was significantly different between UL and AL, between UL and LMS, and between AL and LMS. The combined immunohistochemistry and clinical findings score (total score) was also significantly different between pathological types. The findings of this study suggest that the accuracy of the preoperative diagnosis of SMTs may be improved by using a combination of immunohistochemical and clinical findings.

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Section: Discussionmentioning

confidence: 63%

“…The sections were counterstained with Mayer's hematoxylin. The specificity of the IHC reactions was checked by omitting the primary antibody [8]. …”

Section: Methodsmentioning

confidence: 99%

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Preoperative Diagnosis of Usual Leiomyoma, Atypical Leiomyoma, and Leiomyosarcoma

Matsuda

Ichimura

Kasai

et al. 2014

Sarcoma

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“…In the present study, CD34-stained vessels were counted for each specimen at high-power fields (magnification, x40) and the highest number of positive vessels was recorded. We found that ~80% of leiomyosarcomas exhibit >10 CD34-stained vessels in necrotic areas and that the highest combined sensitivity and specificity is achieved by setting the cut-off score at 10 CD34-stained vessels (8). None of the smooth muscle tumors of uncertain malignant potential examined in the hospital of Osaka City University have exhibited coagulative tumor cell necrosis, thus, this combination is likely to be rare.…”

Section: Discussionmentioning

confidence: 80%

Examination of the use of needle biopsy to perform laparoscopic surgery safely on uterine smooth muscle tumors

et al. 2018

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Abstract. The warning statement issued by the United States Food and Drug Administration against the use of laparoscopic power morcellators prompted a discussion about the methods of preoperative diagnosis of uterine myometrial lesions. Since 1994, transcervical needle biopsies have been performed to differentiate between uterine leiomyomas and leiomyosarcomas. Needle biopsies are also useful for performing laparoscopic surgery on uterine smooth muscle tumors with histopathological safety. In the present study, data from hematoxylin and eosin (HE)-stained specimens obtained by transcervical needle biopsies from 331 patients with smooth muscle tumors and high intensity regions on T1 weighted images (WI) and/or T2WI from magnetic resonance imaging (MRI) scans were retrospectively examined. From a total of 10 patients with moderate or severe cytological atypia, 4 exhibited smooth muscle tumors of uncertain malignant potential and 6 exhibited leiomyosarcomas. The final diagnosis in 3 patients with ≥10 mitotic figures/high-power field was leiomyosarcoma. A total of 5 patients with coagulative tumor cell necrosis exhibited final diagnoses of leiomyosarcoma. Patients without cytological atypia, mitotic figures or coagulative tumor cell necrosis were not diagnosed with either leiomyosarcomas or smooth muscle tumors of uncertain malignant potential. The present study revealed that laparoscopic surgery is safe when HE-stained specimens obtained by transcervical needle biopsy from areas of high intensity on an MRI scan are negative for all three criteria assessed-cytological atypia, mitotic figures and coagulative tumor cell necrosis.

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“…For angiogenesis immunoreaction, we used the CD34 monoclonal mouse antibody (Dako, 1:50 dilution) diluted in 1% BSA in PBS. We defined the scores of CD34 expression as negative, 0 points (Grade 0), mild 1 point (Grade 1), moderate 2 points (Grade 2), and severe 3 points (Grade 3) [ 28 ]. The intensity of staining was recorded as weak, moderate, and strong [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Angiogenesis activity of Jatropha curcas L. latex in cream formulation on wound healing in mice

et al. 2018

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Aim:The aim of this research was to determine the angiogenesis activity of Jatropha curcas latex in cream formulation on CD34 immune expression during wound healing phase in mice skin.Materials and Methods:Amount of 36 2-month-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. All mice were divided into four groups, namely the base cream as control group (A), sulfadiazine 0.1% cream (B), Jatropha curcas latex cream 10% (C), and J. curcas latex cream 15% (D). All groups were treated entire surface of wound. All experiments were performed twice a day for 10 days. Experiments were terminated on days 3, 7, and 10, respectively. The wound healing was assayed in stained histological section in immunohistochemical of the wounds. The CD34 expression was investigated under a microscope.Results:The results showed that the cream from 10% and 15% latex J. curcas revealed moderate immune reaction to CD34 on days 3 and 7 in wound healing of mice skin.Conclusion:We concluded that the cream from 10% and 15% latex J. curcas has potential as angiogenesis activity in wound healing of mice skin.

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A scoring system for histopathologic and immunohistochemical evaluations of uterine leiomyosarcomas

Cited by 12 publications

References 11 publications

Preoperative Diagnosis of Usual Leiomyoma, Atypical Leiomyoma, and Leiomyosarcoma

Preoperative Diagnosis of Usual Leiomyoma, Atypical Leiomyoma, and Leiomyosarcoma

Examination of the use of needle biopsy to perform laparoscopic surgery safely on uterine smooth muscle tumors

Angiogenesis activity of Jatropha curcas L. latex in cream formulation on wound healing in mice

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