A <scp>MYB</scp>‐type transcription factor, <scp>MYB</scp>2, represses light‐harvesting protein genes in <i>Cyanidioschyzon merolae</i>

Kawase, Yasuko; Imamura, Sousuke; Tanaka, Kan

doi:10.1002/1873-3468.12763

Cited by 4 publications

(3 citation statements)

References 26 publications

(41 reference statements)

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“…Amongst the seven nucleus-encoded chloroplast RNA polymerase sigma factors (Gasu_29800, Gasu_17390, Gasu_43280, Gasu_54330, Gasu_44980, Gasu_28760, Gasu_35420) found in the DEGs between heterotrophy and phototrophy, two (Gasu_28760, Gasu_35420) were significantly downregulated, which would suggest that they are required for controlling expression of the chloroplast genome in heterotrophy. In Cyanidoschyzon merolae , a red microalga very close to G. sulphuraria , a MYB2 transcription factor represses the expression of the nuclear-encoded chloroplast RNA polymerase sigma factor gene SIG2, which results in the downregulation of the expression of the chloroplast-encoded phycobilisome genes ( Kawase et al, 2017 ). Only one myb-family related factor was found upregulated in heterotrophy (Gasu_46180, +1.12), which could thus be considered as a good candidate for the repression of sigma factors controlling chloroplast-encoded RNA polymerase.…”

Section: Discussionmentioning

confidence: 99%

Cell adaptation of the extremophilic red microalga Galdieria sulphuraria to the availability of carbon sources

et al. 2022

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were analyzed: heterotrophy with glycerol or glucose and phototrophy. This allowed us to understand the transcriptional response of cells to light and dark environments both at the nuclear and chloroplast levels, and to show that transcription of gene families, acquired by horizontal gene transfer, such as sugar, amino acid, or acetate transporters, were involved in the response to the availability of different (in)organic sources.

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Section: Discussionmentioning

confidence: 99%

Cell adaptation of the extremophilic red microalga Galdieria sulphuraria to the availability of carbon sources

et al. 2022

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“…A previous study showed that CPCC mRNA level in the dark was lower than that in the light under photoautotrophic condition (Kawase, Imamura, & Tanaka, 2017). Taking this result into account, we compared the HA-SPT1 protein level between Cp-SPT1 in the dark with glucose and that in the light with glucose and DCMU ( Figure 4b).…”

Section: Increased Gsspt1 Levels Accelerate C Merolae Heterotrophimentioning

confidence: 96%

Integration of a Galdieria plasma membrane sugar transporter enables heterotrophic growth of the obligate photoautotrophic red alga Cynanidioschyzon merolae

et al. 2019

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The unicellular thermoacidophilic red alga Cyanidioschyzon merolae is an emerging model organism of photosynthetic eukaryotes. Its relatively simple genome (16.5 Mbp) with very low‐genetic redundancy and its cellular structure possessing one chloroplast, mitochondrion, peroxisome, and other organelles have facilitated studies. In addition, this alga is genetically tractable, and the nuclear and chloroplast genomes can be modified by integration of transgenes via homologous recombination. Recent studies have attempted to clarify the structure and function of the photosystems of this alga. However, it is difficult to obtain photosynthesis‐defective mutants for molecular genetic studies because this organism is an obligate autotroph. To overcome this issue in C. merolae , we expressed a plasma membrane sugar transporter, Gs SPT 1, from Galdieria sulphuraria , which is an evolutionary relative of C. merolae and capable of heterotrophic growth. The heterologously expressed GsSPT1 localized at the plasma membrane. GsSPT1 enabled C. merolae to grow mixotrophically and heterotrophically, in which cells grew in the dark with glucose or in the light with a photosynthetic inhibitor 3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea ( DCMU ) and glucose. When the GsSPT1 transgene multiplied on the C. merolae chromosome via the URA Cm‐Gs selection marker, which can multiply itself and its flanking transgene, GsSPT1 protein level increased and the heterotrophic and mixotrophic growth of the transformant accelerated. We also found that Gs SPT 1 overexpressing C. merolae efficiently formed colonies on solidified medium under light with glucose and DCMU . Thus, Gs SPT 1 overexpresser will facilitate single colony isolation and analyses of photosynthesis‐deficient mutants produced either by random or site‐directed mutagenesis. In addition, our results yielded evidence supporting that the presence or absence of plasma membrane sugar transporters is a major cause of difference in trophic properties between C. merolae and G. sulphuraria .

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“…Gene expression in higher plants is commonly regulated through the interplay of specific transcription factors and cis-acting elements. Among these, the MYB2 protein stands out, playing a crucial role in regulating processes like secondary metabolism [26,27], the meristem and pollen development [28,29], and response to abiotic stress [30]. Many experiments have been conducted to study Arabidopsis MYB2, a typical R2R3-MYB gene, and have found that it is associated with improved plant resistance to hormone and environmental stress.…”

Section: Atmyb2 Promotes Tolerance To Salt Stress By Improving Antiox...mentioning

confidence: 99%

Overexpression of AtMYB2 Promotes Tolerance to Salt Stress and Accumulations of Tanshinones and Phenolic Acid in Salvia miltiorrhiza

Li,

Zhang,

et al. 2024

IJMS

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Salvia miltiorrhiza is a prized traditional Chinese medicinal plant species. Its red storage roots are primarily used for the treatment of cardiovascular and cerebrovascular diseases. In this study, a transcription factor gene AtMYB2 was cloned and introduced into Salvia miltiorrhiza for ectopic expression. Overexpression of AtMYB2 enhanced salt stress resistance in S. miltiorrhiza, leading to a more resilient phenotype in transgenic plants exposed to high-salinity conditions. Physiological experiments have revealed that overexpression of AtMYB2 can decrease the accumulation of reactive oxygen species (ROS) during salt stress, boost the activity of antioxidant enzymes, and mitigate oxidative damage to cell membranes. In addition, overexpression of AtMYB2 promotes the synthesis of tanshinones and phenolic acids by upregulating the expression of biosynthetic pathway genes, resulting in increased levels of these secondary metabolites. In summary, our findings demonstrate that AtMYB2 not only enhances plant tolerance to salt stress, but also increases the accumulation of secondary metabolites in S. miltiorrhiza. Our study lays a solid foundation for uncovering the molecular mechanisms governed by AtMYB2 and holds significant implications for the molecular breeding of high-quality S. miltiorrhiza varieties.

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A MYB‐type transcription factor, MYB2, represses light‐harvesting protein genes in Cyanidioschyzon merolae

Cited by 4 publications

References 26 publications

Cell adaptation of the extremophilic red microalga Galdieria sulphuraria to the availability of carbon sources

Cell adaptation of the extremophilic red microalga Galdieria sulphuraria to the availability of carbon sources

Integration of a Galdieria plasma membrane sugar transporter enables heterotrophic growth of the obligate photoautotrophic red alga Cynanidioschyzon merolae

Overexpression of AtMYB2 Promotes Tolerance to Salt Stress and Accumulations of Tanshinones and Phenolic Acid in Salvia miltiorrhiza

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