A screen-printed biosensor using pyruvate oxidase for rapid determination of phosphate in synthetic wastewater

Kwan, Roger C.H.; Leung, Hiu Fai; Hon, Phoebe Y.T.; Barford, J. P.; Renneberg, Reinhard

doi:10.1007/s00253-004-1701-8

Cited by 51 publications

(31 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These characteristics make it a very useful enzyme for clinical diagnostic applications and environmental monitor. Combined with peroxidase, it has been used to determine the alanine transaminase (ALT) and aspartate transaminase (AST) in blood [18] and phosphate ion in river water [13] and wastewater [7]. Up to now, however, commercial product of PyOD is usually produced by conventional cultivation of the wild Lactobacillus lantarum or Aerococcus viridans strain and isolation of the secreted enzyme.…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli

Zhao

Wang

Chu

et al. 2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Pyruvate oxidase (PyOD) is a very useful enzyme for clinical diagnostic applications and environmental monitor. Optimization of the fermentation medium for maximization of PyOD constitutively, production by Escherichia coli DH5alpha/pSMLPyOD was carried out. Response surface methodology (RSM) was used to optimize the medium constituents. A 2(6-2) fractional factorial design (first order model) was carried out to identify the significant effect of medium components towards PyOD production. Statistical analysis of results shows that yeast extract, ammonium sulfate and composite phosphate were significant factors on PyOD production. The optimized values of these three factors were obtained by RSM based on the result of a 2(3) central composite rotatable design. Under these proposed optimized medium, the model predicted a PyOD activity of 610 U/L and via experimental rechecking the model, an activity of 670 U/L was attained.

show abstract

Section: Introductionmentioning

confidence: 99%

Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli

Zhao

Wang

Chu

et al. 2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…But most of these analytical techniques are often limited to low sensitivity, less improved detection limits, small sensitivity, negligible interference from real sample matrix, cost and/or fast response time for selective determination of phosphorous species [16][17][18][19][20][21][22][23][24][25][26][27][28]. Among these analytical techniques, CL can be considered as a promising detection system [15].…”

Section: Introductionmentioning

confidence: 99%

Aerosol Hygroscopic Growth as a New Factor for Trace and Ultra-Trace Determination of Phosphorous in Flame Containing Optical Trapping-Cavity Ring-Down Spectroscopy

Doroodmand¹,

Ghasemi²

2017

J Anal Bioanal Tech

View full text Add to dashboard Cite

A new method has been introduced based on aerosol hygroscopic growth as a new factor for trace and ultra-trace determination of phosphorous in flame containing optical trapping-cavity ring down aerosol extinction (emission) spectrometer (OT-CRD-AES). In this study, a cavity ring down has been designed using hydrogen and air as fuel and oxidant during introduction of the aerosols containing phosphorous species using an ultrasonic generator (humidifier) from an acidic solution by a flow rate of N 2 , followed by detection of the Mie scattering using a charged coupling device (CCD) system. Parameters having strong influence during following scattering of the aerosols during their hygroscopic growth inside the humidified flame (H 2 /air), include: influence and amount of Na + as radiation buffer (as light source), flow rates of H 2 , air and N 2 , kind and concentrations of acid, evaluation of the aerosols inside flame, etc. These parameters were optimized using simplex and one at a time methods. Based on the figures of merit under optimized condition, two linear calibration curves with reverse slopes were evaluated between 10.0 -250.0 ng mL -1 and 1.0 -20.0 µg mL -1 with correlation coefficients (R 2 ) the same as 0.999 and 0.998, respectively. The calibration sensitivities were also estimated to 57.46 and 0.348 (a.u), respectively, with detection limit of 5.0 ng mL -1 . The mechanism of the radiation (Mie scattering) was also evidenced based on i) dependency of the scattered radiations to the quantity of an alkali ions such as Na+ as well as the humidity, ii) presence of acceptable correlation between the response of the cavity with turbidometry, iii) observation of blue shift from green (color related to the luminescence of HPO* in H 2 /air flame) to blue (scattered radiation) and finally iv) effect of hydration number during stability and growth of the aerosols inside the flames.No serious interference was evaluated during analysis of at least 500-fold excess of various foreign species. However, the only observed interference was evaluated during introduction of 200-fold excess of SO 4 2-. Good correlation was also evaluated between the results obtained from this technique and ion exchange chromatography during analysis of wastewater samples that clearly revealed the reliability of this method for phosphorous detection and determination at µg mL -1 and ng mL -1 levels.

show abstract

“…However, these methods are time consuming, laborious, require expensive instruments, well trained operators, and often employ potentially carcinogenic chemicals for the analysis5. The drawbacks of these techniques make them not easily to be used for on-line monitoring.…”

mentioning

confidence: 99%

Highly selective and sensitive phosphate anion sensors based on AlGaN/GaN high electron mobility transistors functionalized by ion imprinted polymer

Jia

Chen

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

A novel ion-imprinted electrochemical sensor based on AlGaN/GaN high electron mobility transistors (HEMTs) was developed to detect trace amounts of phosphate anion. This sensor combined the advantages of the ion sensitivity of AlGaN/GaN HEMTs and specific recognition of ion imprinted polymers. The current response showed that the fabricated sensor is highly sensitive and selective to phosphate anions. The current change exhibited approximate linear dependence for phosphate concentration from 0.02 mg L−1 to 2 mg L−1, the sensitivity and detection limit of the sensor is 3.191 μA/mg L−1 and 1.97 μg L−1, respectively. The results indicated that this AlGaN/GaN HEMT-based electrochemical sensor has the potential applications on phosphate anion detection.

show abstract

A screen-printed biosensor using pyruvate oxidase for rapid determination of phosphate in synthetic wastewater

Cited by 51 publications

References 25 publications

Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli

Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli

Aerosol Hygroscopic Growth as a New Factor for Trace and Ultra-Trace Determination of Phosphorous in Flame Containing Optical Trapping-Cavity Ring-Down Spectroscopy

Highly selective and sensitive phosphate anion sensors based on AlGaN/GaN high electron mobility transistors functionalized by ion imprinted polymer

Contact Info

Product

Resources

About