1986
DOI: 10.1099/00222615-22-2-101
|View full text |Cite
|
Sign up to set email alerts
|

A Search for New Group-B Streptococcal Serotypes

Abstract: Summary. New serotypes were sought among 165 clinical isolates of group-B streptococci that were untypable by antisera for the conventional types Ia, Ib, I1 and 111. The strains were tested for sialic acid, an integral component of the group-B streptococcal type-polysaccharides; trypsin-treated bacteria were tested by slideagglutination with the sialic-acid-specific lectin from the snail Cepaea hortensis. Sialic acid was detected in 96 of the strains; in 95 of these, new type antigens were identified serologic… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
15
0

Year Published

1992
1992
2006
2006

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 19 publications
(15 citation statements)
references
References 12 publications
0
15
0
Order By: Relevance
“…The absorbance spectrum of the puri¢ed lectin was found typically for proteins having a speci¢c absorbance at 1 mg ml 31 of 1.0 at pH 7.6 (V max 277.5 nm, V min 251 nm, E V max =V min = 1.87). The puri¢ed lectin agglutinated speci¢-cally only group B streptococci and not group C streptococci as described by Wagner [11,20,22]. Inhibition of the hemagglutination was only possible by N-acetylneuraminic…”
Section: Puri¢cation Of the Lectinsmentioning
confidence: 90%
See 1 more Smart Citation
“…The absorbance spectrum of the puri¢ed lectin was found typically for proteins having a speci¢c absorbance at 1 mg ml 31 of 1.0 at pH 7.6 (V max 277.5 nm, V min 251 nm, E V max =V min = 1.87). The puri¢ed lectin agglutinated speci¢-cally only group B streptococci and not group C streptococci as described by Wagner [11,20,22]. Inhibition of the hemagglutination was only possible by N-acetylneuraminic…”
Section: Puri¢cation Of the Lectinsmentioning
confidence: 90%
“…In the albumin gland of the garden snail Cepaea hortensis two lectins with di¡erent binding speci¢cities were identi¢ed, a sialic acid-binding agglutinin (CHA-I) and an N-acetyl-D-galactosamine-speci¢c lectin (CHA-II) [20]. CHA-I reacts speci¢cally with all encapsulated group B streptococci (Streptococcus agalactiae) [11,22] and promotes also the phagocytosis of these bacteria (lectinophagocytosis) [6]. The speci¢city of this lectin has been reported to the submolecular level [2].…”
Section: Introductionmentioning
confidence: 99%
“…The slides were rotated gently and examined for an agglutination reaction within 1 min; the reaction was recorded as strong agglutination (++), agglutination (+) or no agglutination (2) (Schaefer et al, 1979;Motlova et al, 1986;Swenshon et al, 1998).…”
Section: Methodsmentioning
confidence: 99%
“…The extraction or enzymic treatment of streptococci may be expected to alter lectin aggregation patterns [4,[34][35][36][37][38], as streptococcal surfaces possess both hydrophobic and hydrophilic molecules [36]. Proteases might be expected to remove proteins, leaving exposed PG and group polysaccharides, while sonication would remove adventitiously bound medium components or non-c oval ently bound secreted molecules .…”
Section: Discussionmentioning
confidence: 99%