2014
DOI: 10.1074/mcp.o113.033571
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A Second-generation Protein–Protein Interaction Network of Helicobacter pylori

Abstract: Helicobacter pylori infections cause gastric ulcers and play a major role in the development of gastric cancer. In 2001, the first protein interactome was published for this species, revealing over 1500 binary protein interactions resulting from 261 yeast two-hybrid screens. Here we roughly double the number of previously published interactions using an ORFeome-based, proteome-wide yeast two-hybrid screening strategy. We identified a total of 1515 protein-protein interactions, of which 1461 are new. The integr… Show more

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Cited by 60 publications
(60 citation statements)
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References 73 publications
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“…The presence of both FliY and FliN as switch proteins is a common feature within theproteobacteria and their co-existence could be associated with the adaptation of the motility system in these species (5) (48,49). The idea that the FliY-FliN and FliY-FliM heterodimers form the functional units of the flagellar motor is also supported by the experimental evidence that both complexes interacted with FliH.…”
Section: Discussionsupporting
confidence: 55%
See 1 more Smart Citation
“…The presence of both FliY and FliN as switch proteins is a common feature within theproteobacteria and their co-existence could be associated with the adaptation of the motility system in these species (5) (48,49). The idea that the FliY-FliN and FliY-FliM heterodimers form the functional units of the flagellar motor is also supported by the experimental evidence that both complexes interacted with FliH.…”
Section: Discussionsupporting
confidence: 55%
“…Although T. maritima carries only the fliY and fliM genes, there is an alternative translation initiation site in the fliY gene that allows the expression of both FliY and FliN-like proteins (47). Alternatively, the accumulated protein-protein interaction data in H. pylori and C. jejuni suggest that FliY and FliN might interact further with a different set of proteins (48,49)…”
Section: Discussionmentioning
confidence: 99%
“…For example, even when overlap is computed only for proteins present in both an E. coli AP-MS (17) and an E. coli Y2H (4) interactome, the overlaps are only 13 and 24%, respectively. Such poor agreement could result from the different specificities of each method for detecting classes of PPIs (3,54), high false positive rates (55,56), or high false negative rates. A further complication is that there are many non-functional PPIs that are not under evolutionary constraint but that result because short amino acid sequences that bind at low affinity evolve frequently by chance (57).…”
Section: Discussionmentioning
confidence: 99%
“…Over the last 15 years, protein-protein "interactomes" have been characterized on a genome-wide scale in bacteria and eukaryotes by yeast 2-hybrid (Y2H) 1 and affinity purificationmass spectrometry (AP-MS) screens (1,(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Y2H screens detect binary physical interactions between pairs of proteins expressed in a non-native host, whereas AP-MS detects proteins that co-purify with a tagged protein expressed in the native organism.…”
mentioning
confidence: 99%
“…Proteins were defined as significantly regulated between the different cell states if they fulfilled both thresholds, an average FC exceeding ± 0.5 and a t-test p-value lower than 0.05. Protein-protein interaction data were extracted from Rain et al [50] and Häuser et al [51]. To identify significant regulated biological processes based on all significantly regulated proteins either including or excluding their annotated protein interaction partners the web based bioinformatics tool DAVID was used [52].…”
Section: Discussionmentioning
confidence: 99%