A second polymorphic lens crystallin (LEN-2) in the mouse: Genetic and biochemical analysis of LEN-1 and LEN-2

Skow, Loren C.; Donner, M.; Popp, Raymond A.; Bailiff, E. G.

doi:10.1007/bf00499122

Cited by 10 publications

(2 citation statements)

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“…Previous studies of mouse lens crystallins by analytical isoelectric focusing had identified inherited polymorphisms among LM crystallins, one of which was encoded by a locus, Len-l, on chromosome 1 (Skow, 1982;Skow et al, 1985). Biochemical studies indicated that LEN-1 was a gamma crystallin but did not establish whether the electrophoretic polymorphism originated from amino acid substitution or posttranslational modification of a gamma crystallin polypeptide.…”

Section: Discussionmentioning

confidence: 94%

“…The mouse gamma crystallin genes have recently been mapped with respect to each other and eye lens obsolescence (Elo) (Quinlan et al, 1987) but have not been positioned relative to other gene markers on chromosome 1. An inherited electrophoretic polymorphism in a gamma crystallin polypeptide, designated Len-1, has been located on mouse chromosome 1 about 5 cM distal to Idh-1 (Skow, 1982;Skow et al, 1985) and closely linked to the fibronectin locus (Skow et al, 1985). In this communication, we further examine the linkage relationships of the gamma crystallin gene family to four other gene markers, including Len-1, in the centromerie portion of mouse chromosome 1.…”

Section: Introductionmentioning

confidence: 96%

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Mapping of mouse gamma crystallin genes on chromosome 1

et al. 1988

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Restriction fragments analysis of DNA from mouse-hamster somatic-cell hybrid clones revealed that a mouse gamma crystallin cDNA hybridized to genomic sequences located on mouse chromosome 1. Identification of restriction fragment length polymorphisms (RFLPs) in the gamma crystallin sequences of inbred strains of mice permitted the further localization of the gamma crystallin genes (Cryg) to the proximal region of chromosome 1 closely linked to the loci encoding isocitrate dehydrogenase (Idh-1), a low molecular weight (LM) crystallin protein polymorphism (Len-1), and fibronectin (Fn-1). A single recombinant was observed between Len-1 and an RFLP in the gamma crystallin gene family, consistent with the hypothesis that Len-1 is one of the several structural loci encoding gamma crystallin genes. Len-1 is probably located on the centromeric end of the Cryg gene family. Linkage of Idh-1, Cryg, and Fn-1 in mice extends the syntenic relationship of those loci to the human, bovine, and rodent genomes and may define a chromosomal region that is generally conserved among mammals. The map position of Cryg, near the eye lens obsolescence (Elo) locus, was confirmed by the discovery that the restriction fragment patterns of gamma crystallin sequences differed between strain C3H/HeJ and the congenic anophthalmic mutant strain, C3H.Elo. Therefore, the gamma crystallin genes were cotransferred with the mutant Elo gene in the derivation of C3H.Elo. The results establish that LEN-1 is a marker for the gamma crystallin gene family, position the gamma crystallin gene family relative to other markers on mouse chromosome 1, and provide additional evidence that the Elo mutation is encoded at a locus closely linked to the gamma crystallin gene cluster. This study found no evidence of recombination hot spots within the gamma crystallin gene cluster.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 96%

Mapping of mouse gamma crystallin genes on chromosome 1

et al. 1988

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Assignment of the microtubule-associated protein 2 gene to mouse Chromosome 1

1992

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Electrophoretic variation in low molecular weight lens crystallins from inbred strains of rats

et al. 1985

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Analysis of rat lens soluble proteins by analytical isoelectric focusing detected two inherited electrophoretic differences in low molecular weight (LM) crystallins from inbred strains of rats (Rattus norvegicus). The polymorphic lens crystallins were shown to be similar to a genetically variant LM crystallin, LEN-1, previously described in mice (Mus musculus) and encoded on chromosome 1, at a locus linked to Pep-3 (dipeptidase). Linkage analysis demonstrated that the rat crystallin locus was loosely linked toPep-3 at a recombination distance of 38 +_ 4.5 U. These data suggest the conservation of a large chromosomal region during the evolution of Rodentia and support the hypothesis that the "y-crystallins are evolving more rapidly than c~-or fl-crystallins.

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A second polymorphic lens crystallin (LEN-2) in the mouse: Genetic and biochemical analysis of LEN-1 and LEN-2

Cited by 10 publications

References 19 publications

Mapping of mouse gamma crystallin genes on chromosome 1

Mapping of mouse gamma crystallin genes on chromosome 1

Assignment of the microtubule-associated protein 2 gene to mouse Chromosome 1

Electrophoretic variation in low molecular weight lens crystallins from inbred strains of rats

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