A seed coat-specific promoter for canola

El-Mezawy, Aliaa; Wu, Limin; Shah, Saleh

doi:10.1007/s10529-009-0098-y

Cited by 13 publications

(8 citation statements)

References 18 publications

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“…AtTT10 was mainly expressed in developing siliques and seeds, specifically in the seed coat [15], [16], [20], [30]. To verify the organ-specific function of Brassica TT10 genes, qRT-PCR was used to determine their mRNA levels in different organs of the black-seeded lines from the three Brassica species.…”

Section: Resultsmentioning

confidence: 99%

Gene Silencing of BnTT10 Family Genes Causes Retarded Pigmentation and Lignin Reduction in the Seed Coat of Brassica napus

Zhang

et al. 2013

PLoS ONE

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Yellow-seed (i.e., yellow seed coat) is one of the most important agronomic traits of Brassica plants, which is correlated with seed oil and meal qualities. Previous studies on the Brassicaceae, including Arabidopsis and Brassica species, proposed that the seed-color trait is correlative to flavonoid and lignin biosynthesis, at the molecular level. In Arabidopsis thaliana, the oxidative polymerization of flavonoid and biosynthesis of lignin has been demonstrated to be catalyzed by laccase 15, a functional enzyme encoded by the AtTT10 gene. In this study, eight Brassica TT10 genes (three from B. napus, three from B. rapa and two from B. oleracea) were isolated and their roles in flavonoid oxidation/polymerization and lignin biosynthesis were investigated. Based on our phylogenetic analysis, these genes could be divided into two groups with obvious structural and functional differentiation. Expression studies showed that Brassica TT10 genes are active in developing seeds, but with differential expression patterns in yellow- and black-seeded near-isogenic lines. For functional analyses, three black-seeded B. napus cultivars were chosen for transgenic studies. Transgenic B. napus plants expressing antisense TT10 constructs exhibited retarded pigmentation in the seed coat. Chemical composition analysis revealed increased levels of soluble proanthocyanidins, and decreased extractable lignin in the seed coats of these transgenic plants compared with that of the controls. These findings indicate a role for the Brassica TT10 genes in proanthocyanidin polymerization and lignin biosynthesis, as well as seed coat pigmentation in B. napus.

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Section: Resultsmentioning

confidence: 99%

Gene Silencing of BnTT10 Family Genes Causes Retarded Pigmentation and Lignin Reduction in the Seed Coat of Brassica napus

Zhang

et al. 2013

PLoS ONE

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“…On the other hand, Liang et al (2006) did not detect any activity in roots and leaves. Interestingly, the TT10 promoter construct studied by Liang et al (2006) was also shown to be active in the seed coat of canola (Brassica napus), but activity was restricted to outer integument (El-Mezawy et al, 2009).…”

Section: Flavonoid Polymerization In Seeds: Other Roles For Arabidmentioning

confidence: 99%

Role of Plant Laccases in Lignin Polymerization

Berthet

Thévenin

Baratiny

et al. 2012

Advances in Botanical Research

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Laccases are ubiquitous oxidases present in animals, plants, bacteria, and fungi. In plant species, they occur as large multigenic families. The involvement of peroxidases in lignification is supported by a wealth of literature data. In contrast, the role of laccases in this major plant process is less firmly established. The large number of plant laccases, which argues for a variety of functions in plant development, makes the identification of lignin-specific laccases a challenge. However, in the past decade, the development of new genetic technologies and tools has played a central role towards resolving this issue. In addition, the plant model, Arabidopsis thaliana, has recently provided novel insights about the occurrence of laccases involved in stem lignification. Information about lignin-related laccases is also available from other species, such as poplar, or other organs and tissues, such as seed coats. This review brings a short and cutting edge survey of laccases and lignification.

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“…Testing the promoter activity of well-characterized Arabidopsis genes in Brassicaceae oilseed crop species is an attractive approach which bypasses the requirement of comprehensive transcriptome analysis in the target species to discover candidate promoters. Several Arabidopsis seed coat specific gene promoters including TT10, DP1, GILT [29], BAN [27], and δVPE [28] have been tested in canola [30,[33][34][35]. In this study, we tested the activity of Arabidopsis TT10 and DP1 promoters in two emerging biofuel oilseed crops, pennycress and camelina.…”

Section: Both Attt10 and Atdp1 Drive Gene Expression Predominantly In...mentioning

confidence: 99%

“…Several seed coatspecific genes have been experimentally identified in the model plant Arabidopsis thaliana [23,[27][28][29][30][31][32]. The promoter of some of these genes were shown to be able to drive gene expression in canola (Brassica napus) in a seed coat-specific manner [33][34][35]. Like canola, pennycress and camelina are members of the Brassicaceae family and are closely related to Arabidopsis [36].…”

Section: Introductionmentioning

confidence: 99%

Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina

Li,

Yell,

2023

Plant Methods

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Background Pennycress and camelina are two important novel biofuel oilseed crop species. Their seeds contain high content of oil that can be easily converted into biodiesel or jet fuel, while the left-over materials are usually made into press cake meals for feeding livestock. Therefore, the ability to manipulate the seed coat encapsulating the oil- and protein-rich embryos is critical for improving seed oil production and press cake quality. Results Here, we tested the promoter activity of two Arabidopsis seed coat genes, AtTT10 and AtDP1, in pennycress and camelina by using eGFP and GUS reporters. Overall, both promoters show high levels of activities in the seed coat in these two biofuel crops, with very low or no expression in other tissues. Importantly, AtTT10 promoter activity in camelina shows differences from that in Arabidopsis, which highlights that the behavior of an exogenous promoter in closely related species cannot be assumed the same and still requires experimental determination. Conclusion Our work demonstrates that AtTT10 and AtDP1 promoters are suitable for driving gene expression in the outer integument of the seed coat in pennycress and camelina.

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A seed coat-specific promoter for canola

Cited by 13 publications

References 18 publications

Gene Silencing of BnTT10 Family Genes Causes Retarded Pigmentation and Lignin Reduction in the Seed Coat of Brassica napus

Gene Silencing of BnTT10 Family Genes Causes Retarded Pigmentation and Lignin Reduction in the Seed Coat of Brassica napus

Role of Plant Laccases in Lignin Polymerization

Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina

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