A selective and diagnostic medium for use in the enumeration of Listeria spp. in foods

Netten, P. van; Ven, Annenienke van de; Perales, Ildefonso; Mossel, D. À. A.

doi:10.1016/0168-1605(88)90011-6

Cited by 28 publications

(11 citation statements)

References 32 publications

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“…The resultant filtrate was serially‐diluted in MRD, plated in 1·0 ml aliquots onto Palcam Agar (PA, Oxoid), incubated at 30°C for 48 h, and examined for colonies exhibiting typical Listeria morphology, i.e. grey‐green colonies with a sunken black centre and black halo against a cherry red medium background (Van Netten et al . 1988), to provide an estimate of the number of uninjured Listeria cells present.…”

Section: Methodsmentioning

confidence: 99%

Thermal resistance of wild-type and antibiotic-resistant Listeria monocytogenes in meat and potato substrates

et al. 2001

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Aims: This study aimed to elucidate the relationship, if any, between the acquisition/ possession of antibiotic resistance in strains of Listeria monocytogenes and the resistance of such strains to heat stress. Methods and Results: D-values calculated using a linear survival model were used to compare the heat resistance of two wild-type (WT) and two antibiotic (streptomycin)-resistant (AR) mutant strains of L. monocytogenes measured in minced beef and potato substrates at 55°C, with and without prior heat shock at 48°C. In both minced beef and potato, no signi®cant differences (P < 0á05) between D-values of AR and WT strains were noted. Heat shock did not signi®cantly increase D-values of WT or AR strains in minced beef, while in potato slices, D-values in almost all cases were signi®cantly higher in samples which had received heat-shock treatment. In minced beef, the use of a non-selective/overlay recovery medium did not result in higher D-values for any strains, while in potato, signi®cantly higher (P < 0á05) D-values were obtained in most cases. Conclusions:The presence or absence of antibiotic resistance genes did not modulate the heat resistance of the strains examined in this study. Signi®cance and Impact of the Study: The study demonstrated that heat shock, and the type of media used to determine bacterial numbers during heat processing, can signi®cantly affect the D-values obtained.

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Section: Methodsmentioning

confidence: 99%

Thermal resistance of wild-type and antibiotic-resistant Listeria monocytogenes in meat and potato substrates

et al. 2001

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“…Because LPM was more selective than MMA, the use of MMA decreased. The introduction of media containing aesculin and ferric citrate as elective system was a further improvement [35,36]. Studies comparing many different plating media have been published since [37–41].…”

Section: Detection Of Listeria Monocytogenesmentioning

confidence: 99%

Listeria monocytogenes: diagnostic problems

Beumer

Hazeleger

2003

FEMS Immunology &Amp; Medical Microbiology

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The first isolation methods for the detection of Listeria spp. were generally based on the direct culture of samples on simple agar media, but isolation of the pathogenic Listeria monocytogenes was difficult. In time, new techniques were developed, based on a variety of selective and elective agents in isolation and enrichment media, which gained better and quicker results. Current reference methods allow the recovery of L. monocytogenes from a variety of foods with relative ease. However, more comparative studies are needed to select one horizontal method. It is suggested that the procedure of the International Organization for Standardization is a good base for such comparisons.

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“…Most published formulations of plating media do not contain any added carbohydrates that can be utilized by L. monocytogenes. Some media do contain esculin as a diagnostic test (17,20). However, it is not used as efficiently as glucose by L. monocytogenes, as pointed out by Swaminathan et al (19).…”

Section: Discussionmentioning

confidence: 99%

“…It obviated the need to incorporate a diagnostic test that could complicate the chemical makeup of LCA. Nevertheless, investigators have had some difficulties with the technique (4,17,20), which is quite cumbersome. Consequently, a modified version of the technique that is more reproducible and convenient than the current version is being developed in this laboratory.…”

Section: Discussionmentioning

confidence: 99%

Selective plating medium for quantitative recovery of food-borne Listeria monocytogenes

Lachica

1990

Appl Environ Microbiol

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A new plating medium (lithium chloride-ceftazidime agar [LCA]) was designed to quantitatively recover food-borne Listeria monocytogenes in the form of large colonies while inhibiting most other food-borne microorganisms. This medium included brain heart infusion agar as the nutritive agar base and a combination of selective agents (LiCl, glycine anhydride, and ceftazidime). Comparison of LCA and lithium chloridephenylethanol-moxalactam agar (LPM) indicated that both were equally effective for the enumeration of the cold-tolerant pathogen in artfficially and naturally contaminated foods. However, LCA was more effective than LPM in the recovery of sublethally heat-injured cells. Moreover, Listeria colonies on LCA exhibited a more distinct bluish hue than those on LPM when viewed by the Henry oblique transillumination technique.

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A selective and diagnostic medium for use in the enumeration of Listeria spp. in foods

Cited by 28 publications

References 32 publications

Thermal resistance of wild-type and antibiotic-resistant Listeria monocytogenes in meat and potato substrates

Thermal resistance of wild-type and antibiotic-resistant Listeria monocytogenes in meat and potato substrates

Listeria monocytogenes: diagnostic problems

Selective plating medium for quantitative recovery of food-borne Listeria monocytogenes

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