A selective medium for the isolation of the acinetobacter genus bacteria

Zebral, Altair A.

doi:10.1590/s0074-02761980000200002

Cited by 3 publications

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Association of the Genus Acinetobacter with the Decomposition of a Swine Carcass and the Isolation and Characterization of a Novel Strain of Acinetobacter sp. P4

et al. 2011

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Using fluorescence in situ hybridization (FISH) and a selective and differential medium, Acinetobacter numbers were enumerated over the time course of decomposition, from fresh to putrid/dry, of a swine carcass. In addition, Acinetobacter diversity and succession were also characterized. Acinetobacter bacterial counts were observed to be the lowest before exposure (undetectable) and increased to their highest during active decay then decreased and leveled during advanced decay through putrid/dry. FISH analysis revealed Acinetobacter cells were mostly clustered together, which is consistent with growth in a non-mixed environment, such as soil. The abundance of Acinetobacter cells decreased from active decomposition to putrid/dry. BLAST analysis using the 16S rRNA-gene sequence identified the isolates as one of the following Acinetobacter spp: A. baumannii, A. haemolyticus, A. junii, A. johnsonii, and A. gerneri. Phenotypic description of the identified isolates closely matched those of known genomic species. One isolate, P4, was observed to be unique in its phenotypic and phylogenetic characteristics and was more closely related to A. sp E10. The isolates from this study displayed multi-antibiotic resistance. The results from the study revealed the association of Acinetobacter spp. with that of carrion which adds to our knowledge of the ecology of this genus along with the potential implications of infection for this opportunistic pathogen.

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Association of the Genus Acinetobacter with the Decomposition of a Swine Carcass and the Isolation and Characterization of a Novel Strain of Acinetobacter sp. P4

et al. 2011

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Draft genome and description of Mixta mediterraneensis strain Marseille-Q2057T sp.nov., a new bacterium isolated from human healthy skin

Antezack

Magnien

Cassir

et al. 2021

New Microbes and New Infections

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In 2019, by culturing a skin swab from the hand of a 30-year-old healthy woman using the culturomic method, we isolated the new bacterial strain Marseille-Q2057 T (= CSUR-Q2057). Matrix-assisted desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) failed to identify this isolate. Analysis of the 16S rRNA gene and Genome-to-Genome comparison suggested that this taxon belongs to a novel bacterial species within the family Erwiniaceae , phylum Proteobacteria. We describe here its main phenotypic characteristics, genome sequence and annotation of Mixta mediterraneensis strain Marseille-Q2057 T , a new member of the Mixta genus, that we propose as type strain.

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Culture Media for Food Spoilage Bacteria of the Order Pseudomonadales: Pseudomonas, Acinetobacter and Psychrobacter spp.

Druggan¹,

Iversen²

2011

Handbook of Culture Media for Food and Water Microbiology

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The common Gram-negative food spoilage bacteria consist primarily of Pseudomonas, Acinetobacter and Psychrobacter spp. The genus Pseudomonas has undergone extensive taxonomic changes in recent years, with species being removed from the order Pseudomonadales and placed in other orders. Selective isolation of Pseudomonadales is facilitated by higher resistance to antimicrobials than other Gram-negative bacteria. Cephaloridine, fucidin and cetrimide can be used to select for Pseudomonas spp., while cefsulodin (a specific inhibitor of Ps. aeruginosa) can be incorporated into media for selection of Acinetobacter spp. Isolation of Psychrobacter spp. is facilitated by their ability to grow at very low temperatures. A fundamental problem in designing media for these organisms is that they have a respiratory metabolism, which does not generate enough acid to lower the pH of media sufficiently to enable differentiation strategies based on sugar metabolism. Differentiation of fluorescent and pigment producing strains is possible on media designed to enhance these traits. Samples with low numbers of competing flora can be examined using non-selective pre-enrichment. However, a selective medium is required for most food and environment samples and a balance needs to be achieved between selectivity, to inhibit competing organisms, and sensitivity, to optimise the recovery of stressed cells. As with all selective media, confirmatory tests need to be made on presumptive positive colonies.

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A selective medium for the isolation of the acinetobacter genus bacteria

Cited by 3 publications

References 0 publications

Association of the Genus Acinetobacter with the Decomposition of a Swine Carcass and the Isolation and Characterization of a Novel Strain of Acinetobacter sp. P4

Association of the Genus Acinetobacter with the Decomposition of a Swine Carcass and the Isolation and Characterization of a Novel Strain of Acinetobacter sp. P4

Draft genome and description of Mixta mediterraneensis strain Marseille-Q2057T sp.nov., a new bacterium isolated from human healthy skin

Culture Media for Food Spoilage Bacteria of the Order Pseudomonadales: Pseudomonas, Acinetobacter and Psychrobacter spp.

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