A selective survey of potato cyst‐nematode pathotypes in Great Britain

Stone, Alan; Holliday, J. M.; Mathias, Paul; Parrott, D. M.

doi:10.1111/j.1365-3059.1986.tb01976.x

Cited by 10 publications

(10 citation statements)

References 21 publications

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“…These include the inhibition of DNA amplification at high concentrations of template DNA (Ibrahim SK, unpublished data), heterogeneity in the ITS sites,14 genetic differences within the species G pallida ,31 genomic variation,32, 33 or the presence of different pathotypes in the same sample 34. 35 The sensitivity and specificity of PCR and ELISA make them obvious choices for determination and quantification of PCN species in field populations.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of PCR, IEF and ELISA techniques for the detection and identification of potato cyst nematodes from field soil samples in England and Wales

Ibrahim

Minnis

Barker

et al. 2001

Pest Management Science

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Effective management of potato cyst nematodes (PCNs) requires simple, rapid and accurate identification and quantification of field populations. Soil samples from a survey of 484 fields in potato rotations in England and Wales were used to compare the identification and quantification of PCNs using IEF, PCR, ELISA and bait plant tests. The cyst counts and bait plant test revealed that 64.3% of field samples contained PCNs. Bait plant tests increased the detection rate of PCNs in field samples by 4-6.4%. This means that some infestations are cryptic and would not normally be detected by standard counts. IEF, PCR and ELISA methods distinguished between Globodera rostochiensis and G pallida and were able to register mixed populations; however they were not in full agreement. All methods suggested that G pallida is the dominant species in the field samples tested. The PCR results indicated that 66% of field samples contained pure G pallida, 8% contained pure G rostochiensis and 26% contained mixtures of the two species. Estimates of the relative process times taken per sample in the PCR, IEF and ELISA techniques are given.

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Section: Discussionmentioning

confidence: 99%

Evaluation of PCR, IEF and ELISA techniques for the detection and identification of potato cyst nematodes from field soil samples in England and Wales

Ibrahim

Minnis

Barker

et al. 2001

Pest Management Science

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“…Certainly the scarcity of cultivars resistant to G. pallida in the UK and Europe reduces the risk of selection for increased virulence in this species (Stone et al, 1986;. However, the limited availability of resistant cul-tivars and the time required for their development raises concerns about the risks associated with the introduction of a wider genetic diversity of PCN, with a potentially greater ability to overcome cultivar resistance.…”

Section: The Significance and Vulnerability Of Resistant Potato Cultimentioning

confidence: 99%

An evaluation of the implications of virulence in non-European populations of Globodera pallida and G. rostochiensis for potato cultivation in Europe

Hockland

Niere

Grenier³

et al. 2012

Nematol

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The potato cyst nematodes Globodera pallida and G. rostochiensis are listed in the EU Plant Health Directive 2000/29/EC and are also subject to the new EU Council Directive 2007/33/EC on the control of potato cyst nematodes, requiring unilateral suppression of these pests in Europe. At the same time there is also pressure to increase world trade in potatoes. Such pressure has to be balanced by the risks involved in the associated spread of these pests and subsequent problems in management. Populations of the potato cyst nematodes from outside Europe, in particular South America, which is considered the origin of G. pallida and G. rostochiensis, pose a risk to those European countries where limited genetic variability of these nematode species has been recorded. The development and usage of resistant cultivars under such conditions has formed a pivotal role in integrated management programmes in Europe. Molecular studies have shown that populations of G. pallida and G. rostochiensis from South America have a different genetic composition from those in Europe. The introduction of such populations would pose a threat to the use of resistant cultivars as a major tool in reducing the potential spread and damage caused by these species. At present, an inability to link precisely genetic variability to the virulence characteristics of a specific nematode population, and quickly identify the virulence status of intercepted populations for inspection purposes, strengthens the case for using plant health legislation to prevent their introduction.

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“…Susceptibility of a number of potato clones to Globodera pallida relative to 'Pentland Javelin', set, at 100% . After Dale (1985), Dale & Phillips (1985), Foot (1978), Fuller & Howard (1977), Parrott & Trudgill (1972), Phillips & Trudgill (1983) and Stone et al . (1986) Kort et al .…”

Section: Definition Of Resistancementioning

confidence: 99%

“…Resistance to G. pallida is said to be polygenically inherited (Stone et al ., 1986) . If so, it is not compatible with easy and unambiguous identification of pathotypes unless a single virulence factor could overcome the collective result of several polygenes, a rather unlikely event as no host-parasite system is known where this has been observed .…”

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confidence: 99%

Pathotype-specificity in potato cyst nematodes, a reconsideration

Nijboer

Parlevliet

1990

Euphytica

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Within the two potato cyst nematode (PCN) species, Globodera rostochiensis and G.. pallida, eight pathotypes are presently discerned by seven differential Solanum clones, whereby resistance is defined as a Pf/Pi ratio < 1 .0, and susceptibility as a ratio above 1 .0 . This pathotyping is felt to be unsatisfactory . Reconsidering the original data and data published by others resulted in the conclusion that there are only three reliably recognizable Ro-pathotypes Rol/Ro4, Ro2/Ro3 and Ro5 . The differential clones 60 .21 .19 and, 65 .346 .19 differentiate only between the two species . Within G. pallida it does not seem possible to identify pathotypes reliably . The resistance-virulence pattern is of a quantitative nature fitting a polygenic system . There appear to be pathotype-specific, effects, which are not large enough to identify and classify pathotypes reliably . It is therefore suggested to rename the Ro-pathotypes into Rol (old Rol and Ro4), Ro3 (old Ro2 and Ro3) and Ro5 (old Ro5) and to speak of more and less virulent Pa-populations .The rigorous requirements for resistance in various countries present great problems to the breeders and result in the removal of much valuable partial resistance . Especially with G. pallida these rigorous requirements are probably an obstacle rather than an encouragement to solve the pallida problem .

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A selective survey of potato cyst‐nematode pathotypes in Great Britain

Cited by 10 publications

References 21 publications

Evaluation of PCR, IEF and ELISA techniques for the detection and identification of potato cyst nematodes from field soil samples in England and Wales

Evaluation of PCR, IEF and ELISA techniques for the detection and identification of potato cyst nematodes from field soil samples in England and Wales

An evaluation of the implications of virulence in non-European populations of Globodera pallida and G. rostochiensis for potato cultivation in Europe

Pathotype-specificity in potato cyst nematodes, a reconsideration

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