A self-assembled, modular DNA delivery system mediated by silica nanoparticles

Luo, Dan; Han, Ernest; Belcheva, Nadya; Saltzman, W. Mark

doi:10.1016/j.jconrel.2003.11.019

Cited by 164 publications

(113 citation statements)

References 9 publications

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“…Substrate-mediated delivery is based on the immobilization of DNA complexes to the culture substrate [9,10,14], resulting in elevated DNA concentrations in the cellular microenvironment, which has been shown to enhance gene delivery [7,33]. While immobilizing DNA to a substrate may seem counterintuitive as cellular internalization is necessary for expression, viral vectors have been shown to associate with the extracellular matrix to facilitate cellular binding and internalization [34,35].…”

Section: Transfection On Bulk Sams On Goldmentioning

confidence: 99%

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

2005

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Gene transfer has many potential applications in basic and applied sciences. In vitro, DNA delivery can be enhanced by increasing the concentration of DNA in the cellular microenvironment through immobilization of DNA to a substrate that supports cell adhesion. Substrate-mediated delivery describes the immobilization of DNA, complexed with cationic lipids or polymers, to a biomaterial or substrate. As surface properties are critical to the efficiency of the surface delivery approach, selfassembled monolayers (SAMs) of alkanethiols on gold were used to correlate surface chemistry of the substrate to binding, release, and transfection of non-specifically immobilized complexes. Surface hydrophobicity and ionization were found to mediate both DNA complex immobilization and transfection, but had no effect on complex release. Additionally, SAMs were used in conjunction with soft lithographic techniques to imprint substrates with specific patterns, resulting in patterned DNA complex deposition and transfection, with transfection efficiencies in the patterns nearing 40%. Controlling the interactions between complexes and substrates, with the potential for patterned delivery, can be used to locally enhance or regulate gene transfer, with applications to tissue engineering scaffolds and transfected cell arrays.

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Section: Transfection On Bulk Sams On Goldmentioning

confidence: 99%

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

2005

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“…Direct delivery of naked DNA can induce transgene expression in vivo; however, plasmids are rapidly degraded by DNase and are rapidly cleared from the tissue [4,5]. Controlled release systems can protect plasmid from nucleases, and a sustained release can replace DNA that is cleared or degraded thereby maintaining elevated levels within the tissue [6][7][8][9][10][11]. These controlled release systems often involve the use of biodegradable polymers to encapsulate the plasmid.…”

Section: Introductionmentioning

confidence: 99%

Intramuscular delivery of DNA releasing microspheres: Microsphere properties and transgene expression

Jang

Shea²

2006

Journal of Controlled Release

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Plasmid-loaded microspheres can provide localized and sustained release into the target tissue, and thus have the potential to enhance the efficiency of naked DNA at promoting transgene expression. In this report, microsphere design parameters are investigated by correlating the extent and duration of transgene expression intramuscularly to the polymer molecular weight and the mass of DNA delivered. Plasmid DNA was incorporated into poly (lactide-co-glycolide) microspheres using a cryogenic double emulsion process, and microspheres were injected intramuscularly. Bolus injection of naked plasmid was used for control, which exhibited transfection of muscle cells with transgene expression that gradually decreased over time. Microspheres fabricated from low molecular weight polymer had expression levels that increased from day 1 to day 92, which subsequently decreased through day 174. Decreasing the microsphere mass delivered resulted in steady expression during the same time. However, microspheres fabricated with high molecular weight polymer had expression for only 14 days. Intramuscular injection resulted in a foreign body response to the microspheres, and these infiltrating cells adjacent were primarily transfected. This understanding of microsphere properties that determine transgene expression and the distribution of transfected cells may facilitate their application to fields such as tissue engineering or DNA vaccines.

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“…Nanoparticles are used to transfer DNA and drugs into animal cell and tissues by the process of endocytosis [7], but the use of nanoparticles for DNA delivery in plants was not possible because the plant cell possess an inflexible cell wall. The nanoparticles which can be used for DNA delivery are zinc, starch, calcium phosphate, carbon materials, silica, gold, magnetite, strontium phosphate, magnesium phosphate and manganese phosphate [8].…”

Section: Transgene Nanovehiclesmentioning

confidence: 99%