A self-assembling peptide hydrogel-based drug co-delivery platform to improve tissue repair after ischemia-reperfusion injury

Liu, Shuyun; Zhao, Meng; Zhou, Yijie; Li, Ling; Wang, Chengshi; Yuan, Yujia; Li, Lan; Liao, Guangneng; Bresette, William; Chen, Younan; Cheng, Jun; Lu, Yanrong; Liu, Jingping

doi:10.1016/j.actbio.2019.12.011

Cited by 77 publications

(59 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mice were randomly divided into four groups (6 per group): the control (Con), I/R, I/R + CUR, and I/R + CUR + ROT groups. The mice were anaesthetized by ip injection of a 1% pentobarbital sodium solution, and the renal artery and vein of the bilateral kidney were occluded for 30 min with a vascular clamp as previously described 20 . For treatment, CUR (50 mg/kg, dissolved in DMSO/0.9% normal saline, v/v = 2:1) was injected into the peritoneal cavity of the mouse, and mice in the I/R group received vehicle (DMSO in 0.9% normal saline).…”

Section: Methodsmentioning

confidence: 99%

“…The mice were anaesthetized by ip injection of a 1% pentobarbital sodium solution, and the renal artery and vein of the bilateral kidney were occluded for 30 min with a vascular clamp as previously described. 20 For treatment, CUR (50 mg/kg, dissolved in DMSO/0.9% normal saline, v/v = 2:1) was injected into the peritoneal cavity of the mouse, and mice in the I/R group received vehicle (DMSO in 0.9% normal saline). To disrupt renal mitochondrial function, ROT (5 mg/kg, dissolved in DMSO/0.9% NS, v/v = 2:3) was injected into the kidneys of the mice with an insulin syringe.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Indispensable role of mitochondria in maintaining the therapeutic potential of curcumin in acute kidney injury

Liu

Zhou

et al. 2021

J Cellular Molecular Medi

Self Cite

View full text Add to dashboard Cite

Acute kidney injury (AKI) is a serious disease for which effective therapeutic agents are required. The capacity of curcumin (CUR) to resolve renal inflammation/oxidative stress and mitochondrial damage has been reported, but crosstalk between these effects and the consequence of this crosstalk remain elusive. In this study, a hypoxia/reoxygenation (H/R)‐induced renal tubular epithelial cell (TEC) injury model and an ischaemia/reperfusion (I/R)‐induced mouse AKI model were treated with CUR with or without mitochondrial inhibitors (rotenone and FCCP) or siRNA targeting mitochondrial transcription factor A (TFAM). Changes in mitochondrial function, inflammation, the antioxidant system and related pathways were analysed. In vitro, CUR suppressed NFκB activation and cytokine production and induced NRF2/HO‐1 signalling in TECs under H/R conditions. CUR treatment also reduced mitochondrial ROS (mtROS) and mitochondrial fragmentation and enhanced mitochondrial biogenesis, TCA cycle activity and ATP synthesis in damaged TECs. However, the anti‐inflammatory and antioxidant effects of CUR in damaged TECs were markedly abolished upon mitochondrial disruption. In vivo, CUR treatment improved renal function and antioxidant protein (NRF2 and SOD2) expression and reduced oxidative stress (8‐OHdG), tubular apoptosis/death, cytokine release/macrophage infiltration and mitochondrial damage in the kidneys of AKI mice. In vitro, the anti‐inflammatory and antioxidant effects of CUR in damaged kidneys were impaired when mitochondrial function was disrupted. These results suggest mitochondrial damage is a driving factor of renal inflammation and redox imbalance. The therapeutic capacity of CUR in kidneys with AKI is primarily dependent on mitochondrial mechanisms; thus, CUR is a potential therapy for various diseases characterized by mitochondrial damage.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Indispensable role of mitochondria in maintaining the therapeutic potential of curcumin in acute kidney injury

Liu

Zhou

et al. 2021

J Cellular Molecular Medi

Self Cite

View full text Add to dashboard Cite

show abstract

“…Its similarity to extracellular matrix (ECM) 96 opens an access to cell delivery therapy and regenerative medicine in histogenesis and tissue repair. Besides, the importance of hydrogels as drug storage vault is of great interest, and according to different purposes, hydrogels of various sizes, structures, and ingredients have been applied in different aspects: tissue regeneration 97 ; anti‐inflammatory 98 ; antimicrobial 99 and antitumor 100 . The introduction of hydrogel to antitumor field has received widespread attention due to its excellent biocompatibility, biodegradability, controllable drug release capacity, and directed therapeutic ability, which observably minimize the toxicity toward normal cells and enhance tumor cell killing efficiency by promoting local chemotherapeutic drug concentration 101 …”

Section: The Classification Preparation Of Hydrogel and Its Advantamentioning

confidence: 99%

Advances and trends of hydrogel therapy platform in localized tumor treatment: A review

Tan

Huang

et al. 2020

J Biomedical Materials Res

View full text Add to dashboard Cite

Due to limitations of treatment and the stubbornness of infiltrative tumor cells, the outcome of conventional antitumor treatment is often compromised by a variety of factors, including severe side effects, unexpected recurrence, and massive tissue loss during the treatment. Hydrogel‐based therapy is becoming a promising option of cancer treatment, because of its controllability, biocompatibility, high drug loading, prolonged drug release, and specific stimuli‐sensitivity. Hydrogel‐based therapy has good malleability and can reach some areas that cannot be easily touched by surgeons. Furthermore, hydrogel can be used not only as a carrier for tumor treatment agents, but also as a scaffold for tissue repair. In this review, we presented the latest researches in hydrogel applications of localized tumor therapy and highlighted the recent progress of hydrogel‐based therapy in preventing postoperative tumor recurrence and improving tissue repair, thus proposing a new trend of hydrogel‐based technology in localized tumor therapy. And this review aims to provide a novel reference and inspire thoughts for a more accurate and individualized cancer treatment.

show abstract

“…HK2 cells (5×10 3 cells/well) were seeded at 37°C overnight. Then, cells were treated with emodin (10,20,40, and 60 μM) for 2 days. After that, 10 μL CCK8 reagent (Beyotime) per well was added for 2 hours.…”

Section: Cck8 Assaysmentioning

confidence: 99%

“…8 Recent reports have indicated that HGF has a significant antifibrotic effect. 9,10 In addition, Rheum officinale has been confirmed to have some bioactivity, including antifibrotic activity. 11,12 Emodin is the major bioactive compound of R. officinale and has strong anti-inflammatory and antioxidant properties.…”

Section: Introductionmentioning

confidence: 99%

<p>Emodin Retarded Renal Fibrosis Through Regulating HGF and TGFβ–Smad Signaling Pathway</p>

Yang

Deng

et al. 2020

DDDT

View full text Add to dashboard Cite

Background: Renal fibrosis is a frequently occurring type of chronic kidney disease that can cause end-stage renal disease. It has been verified that emodin or HGF can inhibit the development of renal fibrosis. However, the antifibrotic effect of emodin in combination with HGF remains unclear. Methods: Cell viability was detected with CCK8. Gene and protein expression in HK2 cells was detected by qRT-PCR and Western blot, respectively. Moreover, a unilateral ureteral obstruction-induced mouse model of renal fibrosis was established for investigating the antifibrotic effect of emodin in combination with HGF in vivo. Results: HGF notably increased the expression of collagen II in TGFβ-treated HK2 cells. In addition, HGF-induced increase in collagen II expression was further enhanced by emodin. In contrast, fibronectin, αSMA and Smad2 expression in TGFβ-stimulated HK2 cells was significantly inhibited by HGF and further decreased by combination treatment (emodin plus HGF). Moreover, we found that combination treatment exhibited better antifibrotic effects compared with emodin or HGF in vivo. Conclusion: These data demonstrated that emodin plus HGF exhibited better antifibrotic effects compared with emodin or HGF. As such, emodin in combination with HGF may serve as a new possibilty for treatment of renal fibrosis.

show abstract

A self-assembling peptide hydrogel-based drug co-delivery platform to improve tissue repair after ischemia-reperfusion injury

Cited by 77 publications

References 38 publications

Indispensable role of mitochondria in maintaining the therapeutic potential of curcumin in acute kidney injury

Indispensable role of mitochondria in maintaining the therapeutic potential of curcumin in acute kidney injury

Advances and trends of hydrogel therapy platform in localized tumor treatment: A review

<p>Emodin Retarded Renal Fibrosis Through Regulating HGF and TGFβ–Smad Signaling Pathway</p>

Contact Info

Product

Resources

About