A Self-Contained Miniaturized PCR System using Electromagnetic Actuators

Chia, Bonnie Tingting; Yang, Sheng-An; Cheng, Ming-Yuan; Lin, Chong; Lin, Chii‐Wann; Yang, Yao‐Joe

doi:10.1109/memsys.2009.4805376

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…In another approach, Chia et al developed fully integrated, portable PCR device that consists of the following four major parts: a disposable chamber chip with microchannels and pumping membranes, a heater chip with microheaters and temperature sensors, a linear array of electromagnetic actuators and a control/sensing circuit. Apart from the small size (67 × 67 × 25 mm 3 ) and less power consumption (5V DC) and reduced volume of DNA solution, this system could effectively reduce the PCR process time into one-third of the time required by typical commercial PCR system [19]. In another approach, Steinbach et al [20] came forward with their K-Ras mutation detection on chip.…”

Section: On-chip Dna Hybridization and Pcrmentioning

confidence: 99%

Cells and Organs on Chip—A Revolutionary Platform for Biomedicine

Joshi¹

2016

Lab-on-a-Chip Fabrication and Application

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Lab-on-a-chip (LOC) and microfluidics are important technologies with numerous applications from drug delivery to tissue engineering. LOC integrates fluidic and electronic components on a single chip and becomes very attractive due to the possibility of their state-of-art implementation in personalized devices for the point-of-care treatments. Microfluidics is the technique that deals with small (10-9 to 10-18 L) amounts of fluids, using channels with dimensions of 10 to 100 μm. These LOC and microfluidics devices enable the development of next-generation portable and implantable bioelectronics devices. Superior chip-based technologies are emerging with the advances in microfluidics and motivating various chip-based methods for rapid lowcost analysis as compared to traditional laboratory method.An organ-on-chip (OOC) is on-chip cell culture device created with microfabrication techniques and contains continuously perfused chambers inhabited by living cells that simulate tissue-and organ-level physiology. In vitro models of cells, tissues and organ based on LOC devices are a major breakthrough for research in biologic systems and mechanisms. The recapitulations of cellular events in OOC devices provide them an edge over twodimensional (2D) and three-dimensional (3D) cultures and open a gateway for their newer applications in biomedicine such as tissue engineering, drug discovery and disease modeling. In this chapter, the advancement and potential applications of OOC devices are discussed. Keywords: lab-on-chip, MEMS, organ-on-chip, 3D cell culture, drug discovery 1. Introduction: why cell and organ on chip? The field of microfluidics or lab-on-chip (LOC) technology aims to advance and broaden the possibilities of bioassays, cell biology and biomedical research based on the idea of miniaturi

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Section: On-chip Dna Hybridization and Pcrmentioning

confidence: 99%

Cells and Organs on Chip—A Revolutionary Platform for Biomedicine

Joshi¹

2016

Lab-on-a-Chip Fabrication and Application

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“…The PCR thermal cycling reactions consist of the periodical repetition of three different temperatures (denaturation, annealing and extension temperature) [3]. In the three phases the usual reaction temperatures were 94℃, 55℃, 72℃.…”

Section: Introductionmentioning

confidence: 99%

A Design of DSPIC Based Microfluidic PCR Chip Temperature Controlling System

Wang

Song

Tan

et al. 2011

AMM

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In this paper a dsPIC microcontroller based temperature control system is developed for polymerase chain reaction (PCR) on-chip. PCR is one of the most important techniques in molecular biology and temperature control is the key technique of PCR. Yet, most of the works are based on PC, through an RS 232 interface to set and control the temperature. Here we design a temperature control system based on DSPIC Microcontroller. It can be much portable and cheaper than PC based systems. The system configuration mainly consists of a high precision, stable performance sensor PT1000, a signal amplification circuit, a PID algorithm. And then, we use MAX1978 to improve the accuracy of temperature control. After that we choose thermoelectric cooler (TEC) modules as actuator to improve system heating and cooling rate. It is believe that the portable PCR temperature control system will play an important role in the development of the Microfluidic PCR.

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A Self-Contained Miniaturized PCR System using Electromagnetic Actuators

Cited by 2 publications

References 8 publications

Cells and Organs on Chip—A Revolutionary Platform for Biomedicine

Cells and Organs on Chip—A Revolutionary Platform for Biomedicine

A Design of DSPIC Based Microfluidic PCR Chip Temperature Controlling System

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