A self-healing and bioactive coating based on duplex plasma electrolytic oxidation/polydopamine on AZ91 alloy for bone implants

Farshid, Safoora; Kharaziha, Mahshid; Atapour, Masoud

doi:10.1016/j.jma.2022.05.020

Cited by 40 publications

(14 citation statements)

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“…The observed phenomenon could be attributed to the enlargement of the discharge diameter over time during the PEO process, resulting in the formation of larger pores and an increase in the roughness of the HA/PEO-coated sample. Since a surface roughness of 0.5–1.5 μm could improve cell adhesion and bone regeneration, F0.8-D50-40-HA and F1-D50-40-HA samples might be considered suitable candidates for bone tissue applications. Furthermore, based on the water contact angle results (Figure D), it was evident that HA coating significantly enhanced the wettability of WE43, which could be attributed to HA-containing OH groups .…”

Section: Resultsmentioning

confidence: 99%

“…Cell responses varied based on surface morphology, chemistry, corrosion resistance, water contact angle, and roughness. The WE43 sample exhibited minimal cell spreading with predominantly round-shaped cells after 1 and 3 days of culture, likely due to its reduced corrosion resistance leading to the release of hydrogen and fluctuations in pH levels …”

Section: Resultsmentioning

confidence: 99%

“…The WE43 sample exhibited minimal cell spreading with predominantly round-shaped cells after 1 and 3 days of culture, likely due to its reduced corrosion resistance leading to the release of hydrogen and fluctuations in pH levels. 23 In contrast, the F0.8-D70-40-HA sample displayed a higher cell density than that of the WE43 sample. Nevertheless, cells in this sample retained their spherical shape initially.…”

Section: Electrochemical Properties Of Ha/peo Coatingsmentioning

confidence: 89%

“…Subsequently, samples were meticulously abraded using SiC papers and subjected to ultrasonic cleaning with water and acetone. As described in prior studies 23 the PEO process utilized a water-based electrolyte comprising 10 g/L Na 3 PO 4 and 2 g/L KOH within a stainless-steel bath. The optimal applied voltage and current density were determined to be 420 V and 1.5 A/cm 2 , respectively, based on previously established criteria 23 .…”

Section: Methodsmentioning

confidence: 99%

“…As described in prior studies 23 the PEO process utilized a water-based electrolyte comprising 10 g/L Na 3 PO 4 and 2 g/L KOH within a stainless-steel bath. The optimal applied voltage and current density were determined to be 420 V and 1.5 A/cm 2 , respectively, based on previously established criteria 23 . The parameters, namely, frequency, duty cycle, and deposition time, were systematically varied to elucidate the impact of altering PEO parameters on the ultimate morphology of the HA/PEO coatings, as outlined in Table 1.…”

Section: Methodsmentioning

confidence: 99%

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Morphology-Dependent Immunomodulatory Coating of Hydroxyapatite/PEO for Magnesium-Based Bone Implants

Farshid,

Kharaziha,

Salehi

et al. 2023

ACS Appl. Mater. Interfaces

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One of the most critical issues concerning orthopedic implants is the risk of chronic inflammation, which poses a threat to the bone healing process. Osteo-immunomodulation plays a pivotal role in implant technology by influencing proinflammatory and anti-inflammatory responses, ultimately promoting bone healing. This study aims to investigate the morphology-dependent osteo-immunomodulatory properties of a hydroxyapatite (HA)/plasma electrolytic oxidation (PEO)-coated WE43 alloy. In this context, following the PEO process with various operational parameters (duty cycles of 50–40, 50–20, 70–40%, and frequencies of 0.5, 0.8, and 1 kHz), a layer of HA was applied as the top coating using a straightforward hot-dip process. The results revealed the formation of the PEO layer with distinct morphologies and pore sizes, depending on the operational parameters. Specifically, a uniform PEO coating with small pore sizes (5.2–5.3 μm) led to the creation of plate-like HA particles, while a random-like HA structure formed on nonuniform surfaces with large pores (7.0–11.1 μm) of PEO. Moreover, it was observed that the plate-like HA coating exhibited higher adhesion strength than the random one (classified as class 2 vs class 3 based on cross-cut standards). Furthermore, electrochemical impedance spectroscopy (EIS) and polarization studies confirmed a substantial increase in the polarization resistance (680 kΩ) and total impedance (48 559.6 Ω) for the plate-like HA/PEO as compared to the substrate (an increase of 1511-fold and 311-fold, respectively) and the random HA/PEO samples (an increase of 85-fold and 18-fold, respectively). In addition, compared to random HA coatings, there was a significant enhancement in the viability (150% control vs 96% control), proliferation, and differentiation of MG63 cells when exposed to plate-like HA coatings. Moreover, surface morphology and chemistry pronouncedly impacted macrophages’ viability, morphology, and phenotype. Notably, plate-like HA coatings resulted in a higher upregulation of BMP-2 and TGF-β than proinflammatory cytokines (IL-6 and M-CSF), indicating a polarization of macrophage type 1 (M1) toward type 2 (M2). In summary, the bilayer HA/PEO coating exhibited remarkable osteo-immunomodulatory activity, making it highly appealing for use in bone implant applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Electrochemical Properties Of Ha/peo Coatingsmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Morphology-Dependent Immunomodulatory Coating of Hydroxyapatite/PEO for Magnesium-Based Bone Implants

Farshid,

Kharaziha,

Salehi

et al. 2023

ACS Appl. Mater. Interfaces

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Hierarchical Integration of Curcumin‐Loaded CaCO₃ Nanoparticles and Black Phosphorus Nanosheets in Core/Shell Nanofiber for Cranial Defect Repair

Zheng,

Tan,

Chen

et al. 2024

Adv Healthcare Materials

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Reconstruction and healing of large craniofacial bone defects are major clinical challenges due to high risk of chronic inflammation and reduced cell mineralization levels. Herein, a core‐shell nanofiber‐based implant with significant pro‐osteogenesis capability for treating skull defects is reported, which is hierarchically integrated with curcumin‐loaded calcium carbonate nanoparticles (CaCO3@Cur NPs) in the outer layers and black phosphorus nanosheets (BPNSs) in the core compartments. The radical alignment of the integrated nanocomponents allows the sequential in situ release of the therapeutic agents in a controlled manner after implantation. Curcumin can repolarize M1 macrophages into M2 phenotypes for anti‐inflammation purposes. Meanwhile, the released calcium and phosphate ions can promote the biomineralization of hydroxyapatite at the defect site and facilitate bone regeneration. Evaluations on cranial defect‐bearing rat models demonstrated that the electrospun fibers in the present study substantially promoted restoration of the damaged skulls and inhibited inflammation in the wound bed. This strategy provides a new idea for the treatment of skull defects in the clinic.

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Characterization and In Vitro Behavior of PEO Coated Mg Modified with Antibacterial Ag(I) and Cu(II) Complexes

Tsimafeyeva,

Starykevich,

Snihirova

et al. 2024

Chemistry A European J

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The use of Mg‐based biomaterials with a number of their advantageous properties are overshadowed by uncontrollable metal corrosion. Moreover, the use of implants goes alongside with the threat of pathogens‐associated complications. In this study, PEO coated Mg biomaterial loaded with antibacterial Ag(I) and Cu(II) complexes is produced and tested to meet both appropriate protective characteristics as well as sufficient level of antibacterial activity. To achieve a suitable level of anticorrosion protection phosphate and fluoride‐phosphate electrolytes are used in the PEO process. Investigation of the surface thickness and morphology done by means of cross‐section analysis and scanning electron microscopy (SEM), as well as electrochemical impedance spectroscopy (EIS) assay show precedence of the fluoride containing PEO coating and make it the material of choice for further modification with Ag(I) and Cu(II) complexes. The presence of the complexes on the PEO surface is confirmed by energy dispersive X‐ray spectroscopy (EDX). X‐ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and glow discharge optical emission spectroscopy (GDOES) are used to estimate the complexes’ chemical state and depth of penetration in the coating surface. Based on the results of antibacterial assay, the modified coatings are found to be active against both Gram‐positive and Gram‐negative bacteria.

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A self-healing and bioactive coating based on duplex plasma electrolytic oxidation/polydopamine on AZ91 alloy for bone implants

Cited by 40 publications

References 74 publications

Morphology-Dependent Immunomodulatory Coating of Hydroxyapatite/PEO for Magnesium-Based Bone Implants

Morphology-Dependent Immunomodulatory Coating of Hydroxyapatite/PEO for Magnesium-Based Bone Implants

Hierarchical Integration of Curcumin‐Loaded CaCO₃ Nanoparticles and Black Phosphorus Nanosheets in Core/Shell Nanofiber for Cranial Defect Repair

Characterization and In Vitro Behavior of PEO Coated Mg Modified with Antibacterial Ag(I) and Cu(II) Complexes

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A self-healing and bioactive coating based on duplex plasma electrolytic oxidation/polydopamine on AZ91 alloy for bone implants

Cited by 40 publications

References 74 publications

Morphology-Dependent Immunomodulatory Coating of Hydroxyapatite/PEO for Magnesium-Based Bone Implants

Morphology-Dependent Immunomodulatory Coating of Hydroxyapatite/PEO for Magnesium-Based Bone Implants

Hierarchical Integration of Curcumin‐Loaded CaCO3 Nanoparticles and Black Phosphorus Nanosheets in Core/Shell Nanofiber for Cranial Defect Repair

Characterization and In Vitro Behavior of PEO Coated Mg Modified with Antibacterial Ag(I) and Cu(II) Complexes

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Product

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Hierarchical Integration of Curcumin‐Loaded CaCO₃ Nanoparticles and Black Phosphorus Nanosheets in Core/Shell Nanofiber for Cranial Defect Repair