A Self‐Powered Piezo‐Bioelectric Device Regulates Tendon Repair‐Associated Signaling Pathways through Modulation of Mechanosensitive Ion Channels (Adv. Mater. 40/2021)

Fernández‐Yagüe, Marc A.; Trotier, Alexandre; Demir, Seçil; Abbah, Sunny Akogwu; Larrañaga, Aitor; Thirumaran, Arun; Stapleton, Aimee; Tofail, Syed A. M.; Palma, Matteo; Kilcoyne, Michelle; Pandit, Abhay; Biggs, Manus

doi:10.1002/adma.202170315

Cited by 5 publications

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“…The protein–antibody array was constructed as previously described. [ 9,159–162 ] A total of 42 commercial antibodies (see Table S2, Supporting Information, for catalogue numbers and dilution detail) were buffer‐exchanged with PBS and quantified using the Pierce BCA Protein Assay Kit (23227, Thermo Scientific, Waltham, USA). Approximately 1 nL was printed per feature on Nexterion H amine‐reactive hydrogel‐coated glass slides using a SciFLEXARRAYER S3 piezoelectric printer (Scienion, Berlin, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Prior to use, the antibody microarray slides were allowed to equilibrate to RT for 30 min under desiccant. Fluorescently labeled protein lysates were incubated on microarrays as previously described [ 159–161,163 ] with limited light exposure throughout the process. Initially, two labeled samples were titrated (2.5 to 10 µg mL −1 ) to determine the optimal concentration for all samples to obtain an extractable, nonsaturated signal response (i.e., N1000 and 65 000 relative fluorescence units (RFU)) with low background (b500 RFU) for all samples (Figure S5, Supporting Information).…”

Section: Methodsmentioning

confidence: 99%

“…Microarray data extraction was performed as previously described. [ 159,160,162,164 ] In short, GenePix Pro v6.1.0.4 (Molecular Devices, Berkshire, UK) was used to extract raw intensity values from image files using a proprietary *.gal file which enabled the identification of 230 µm printed protein spots using adaptive diameter (70–130%) circular alignment. The data were then exported to Excel (version 2010, Microsoft).…”

Section: Methodsmentioning

confidence: 99%

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Micromotion Derived Fluid Shear Stress Mediates Peri‐Electrode Gliosis through Mechanosensitive Ion Channels

et al. 2023

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The development of bioelectronic neural implant technologies has advanced significantly over the past 5 years, particularly in brain–machine interfaces and electronic medicine. However, neuroelectrode‐based therapies require invasive neurosurgery and can subject neural tissues to micromotion‐induced mechanical shear, leading to chronic inflammation, the formation of a peri‐electrode void and the deposition of reactive glial scar tissue. These structures act as physical barriers, hindering electrical signal propagation and reducing neural implant functionality. Although well documented, the mechanisms behind the initiation and progression of these processes are poorly understood. Herein, in silico analysis of micromotion‐induced peri‐electrode void progression and gliosis is described. Subsequently, ventral mesencephalic cells exposed to milliscale fluid shear stress in vitro exhibited increased expression of gliosis‐associated proteins and overexpression of mechanosensitive ion channels PIEZO1 (piezo‐type mechanosensitive ion channel component 1) and TRPA1 (transient receptor potential ankyrin 1), effects further confirmed in vivo in a rat model of peri‐electrode gliosis. Furthermore, in vitro analysis indicates that chemical inhibition/activation of PIEZO1 affects fluid shear stress mediated astrocyte reactivity in a mitochondrial‐dependent manner. Together, the results suggest that mechanosensitive ion channels play a major role in the development of a peri‐electrode void and micromotion‐induced glial scarring at the peri‐electrode region.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Micromotion Derived Fluid Shear Stress Mediates Peri‐Electrode Gliosis through Mechanosensitive Ion Channels

et al. 2023

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“…6a). 129 Electrical stimulation is one of the most commonly used stimuli. 128 Recently, it has been found that Col I in tendon has piezoelectric properties, which has prompted people to conduct in-depth research on the role of bioelectricity in tissue repair.…”

Section: Stimulus-responsive Nanofibrous Scaffoldsmentioning

confidence: 99%

“…6b). 129 To simulate the layered structure of the tendon collagen fibers, the collector velocity was controlled between 4.2 m s À1 and 29.3 m s À1 (Fig. 6c).…”

Section: Stimulus-responsive Nanofibrous Scaffoldsmentioning

confidence: 99%

Nanofibrous scaffolds for the healing of the fibrocartilaginous enthesis: advances and prospects

Li¹,

Ren²,

Xue³

et al. 2023

Nanoscale Horiz.

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Highly Stretchable Piezoelectric Elastomer for Accelerated Repairing of Skeletal Muscles Loss

Fang,

Wang,

Lin

et al. 2024

Adv Funct Materials

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Skeletal muscle loss is a common clinical disease, whose long treatment period and expensive treatment costs bring a heavy burden to patients. Currently, the use of piezoelectric materials to generate in situ and efficient electrical stimulation has been proven to significantly promote wound healing and other defects. However, skeletal muscle requires repaired materials with high elastic deformation to accompany its reciprocating movement during the healing process. Therefore, developing biocompatible materials that combine high piezoelectricity and stretchability is a great challenge. In the present work, a piezoelectric elastomer PPBE is prepared by copolymerization of bio‐based diacid and diol. PPBE has matching elastic modulus with the skeletal muscle to accompanied proceed stretching and recovery movements. Piezoelectrical charge from PPBE scaffold under ultrasound and mechanical stimuli could promote myogenic differentiation via Ca2+ signaling pathway and modulate cell functions, resulting in improved muscle morphology and functional performance of skeletal muscle. Thus, development of the degradable PPBE scaffold would provide new therapy strategy for volumetric muscle loss (VML) and present great potential in repairing of other tissues.

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A Self‐Powered Piezo‐Bioelectric Device Regulates Tendon Repair‐Associated Signaling Pathways through Modulation of Mechanosensitive Ion Channels (Adv. Mater. 40/2021)

Cited by 5 publications

References 0 publications

Micromotion Derived Fluid Shear Stress Mediates Peri‐Electrode Gliosis through Mechanosensitive Ion Channels

Micromotion Derived Fluid Shear Stress Mediates Peri‐Electrode Gliosis through Mechanosensitive Ion Channels

Nanofibrous scaffolds for the healing of the fibrocartilaginous enthesis: advances and prospects

Highly Stretchable Piezoelectric Elastomer for Accelerated Repairing of Skeletal Muscles Loss

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