A semi-automated procedure for the assay of 23 enzymes from Drosophila melanogaster

Stam, Lynn F.; Laurie-Ahlberg, C. C.

doi:10.1016/0020-1790(82)90023-3

Cited by 24 publications

(18 citation statements)

References 32 publications

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“…16) and from the slow development line (lanes [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. Note the prevalence oflarge rDNA spacers (6.8-9.5 kb) having high copy number (dark intensity) in samples from the fast line (lanes [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] The weighted mean emergence time of all flies (n = 8,775) in the fast line was 11.19 days, whereas the flies (n = 6,530) in the slow line emerged in 11.74 days. Thus, flies in the fast line emerged about 13 hr earlier, on the average, than flies in the slow line; t tests showed that differences between the fast and slow lines were significant (P < 0.001) in all three experiments.…”

Section: Resultsmentioning

confidence: 99%

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Correlations between development rates, enzyme activities, ribosomal DNA spacer-length phenotypes, and adaptation in Drosophila melanogaster.

Cluster¹,

Marinković²,

Allard³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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Correction. In reference to the article "Correlations between development rates, enzyme activities, ribosomal DNA spacer-length phenotypes, and adaptation in Drosophila melanogaster" by P. D. Cluster, D. Marinkovic, R. W. Allard, and F. J. Ayala, which appeared in number 2, January 1987, of Proc. Natl. Acad. Sci. USA (84,[610][611][612][613][614], the following error should be noted. The first author's initials are correctly cited on the paper (P. D. Cluster) but are incorrect in the index (p. v) and the Contributed by R. W. Allard, September 26, 1986 ABSTRACT Selection for "fast" preadult development rate among the progeny offlies collected in a natural population of Drosophila melanogaster produced a line that developed more rapidly than a line selected for "slow" preadult development rate. Assays for enzyme activity levels showed that the activities of a-glycerophosphate dehydrogenase, alcohol dehydrogenase, and malic enzyme were higher in the fast than in the slow line, but that the activity of superoxide dismutase was lower in the fast line. Differences in the frequencies of spacerlength phenotypes of X chromosome-linked rRNA genes (rDNA), which developed between the lines during the selection process, are larger than can be explained on the basis of genetic drift alone. Long rDNA spacers had high frequency in the fast line; short spacers, in the slow line. We conclude that enzyme levels affected adaptation under the selective regimes imposed and that the different X-linked rDNA spacer-length phenotypes are either adaptive in themselves or that they mark chromosomal segments carrying genes relevant to adaptation.Interest in the role that regulatory genes play in adaptation has increased in recent years. In insects, regulatory variation for enzyme activities has frequently been found both within and among individuals (1-6); thus, for example, variation in the activity of six among seven enzymes studied has been reported to be correlated with preadult development rates in Drosophila melanogaster and Drosophila subobscura (7,8). Although allozyme variants have been implicated as sources of variation for enzyme activities (2,5,7,8) and for development rates (7-9), variation in enzyme activity has also been reported in stocks that are identical to each other for the chromosome carrying the structural locus but differ in other chromosomes, which demonstrates that regulatory genes other than structural allozymes may also be responsible for variation in enzyme activities (1, 5).rRNA gene (rDNA) mutants have been reported to be associated with retarded development in D. melanogaster (10), Drosophila hydei (11), and Drosophila mercatorum (12, 13), and it has been suggested that the slower rates of development may be due to decreased metabolic activity resulting from reduced numbers of functional rRNA genes. The transcriptional efficiency of the rDNA might also affect development rates. There is evidence that rDNA spacers may modify this transcriptional efficiency (14)(15)(16)(17)(18)(19) P.D.C., R.W.A., and A. A....

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Section: Resultsmentioning

confidence: 99%

“…Enzyme assays were performed as described (3,4) at 300C in 0.1 ml of homogenate per 0.9 ml of reaction mixture in a Gilford model 250 spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Correlations between development rates, enzyme activities, ribosomal DNA spacer-length phenotypes, and adaptation in Drosophila melanogaster.

Cluster¹,

Marinković²,

Allard³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…This result might be explained as the possible consequence of the egg production processes at metabolic level. Also, a large amount of variation was observed in assays of D. melanogaster females, and it was preferable to use males for enzyme assay procedures (Stam and Laurie-Ahlberg, 1982). In this study we analysed males in all other experiments.…”

Section: Resultsmentioning

confidence: 99%

“…The assay procedures have been described by Avise and McDonald (1976), Stam and Laurie-Ahlberg (1982), and Marinkovi6 et al (1984b). The homogenization buffer was 0.01 M KH 2 P0 4 , 1 mM EDTA, pH 7.4.…”

Section: Methodsmentioning

confidence: 99%

Activity of enzymes and fitness variation

Milošević¹,

Marinković²

1989

Genet Sel Evol

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“…The assay procedures have been described by Avise and McDonald (1976), Stam and Laurie-Ahlberg (1982), and Marinkovi6 et al (1984b Flies from the 1985 sample were also used for electrophoretic analysis of 7 gene-enzyme systems, i.e. of G-6pdh, 6-Pgdh, a-Gpdh, Adh, Hk, Me, and Idh.…”

Section: Introductionmentioning

confidence: 99%