A semi-automatic and quantitative method to evaluate behavioral photosensitivity in animals based on the optomotor response (OMR)

Matsuo, Megumi; Ando, Yumiko; Kamei, Yasuhiro; Fukamachi, Shoji

doi:10.1242/bio.033175

Cited by 12 publications

(31 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the apparent effects on mate choice, blue light sensitivity of the sws2 mutants was equivalent to that of the wild type (Figure 4). By contrast, in our previous study, an apparent reduction in photopic red light sensitivity could be detected in the lws mutants (Homma et al, 2017;Matsuo et al, 2018). These potentially controversial results are not at all surprising because whereas red light (λ > 740 nm) could only be absorbed by LWSs (λ max = 561-562 nm), blue light tested in this study (λ = 380-500 nm) could likely be absorbed by not only SWS2s (λ max = 405-439 nm) but also the neighboring SWS1 (λ max = 356 nm) and RH2s (λ max = 452-516 nm) (Matsumoto et al, 2006).…”

Section: Apparent Effects Of the Sws2 Mutations On Mate Choice But Nocontrasting

confidence: 85%

“…These potentially controversial results are not at all surprising because whereas red light (λ > 740 nm) could only be absorbed by LWSs (λ max = 561-562 nm), blue light tested in this study (λ = 380-500 nm) could likely be absorbed by not only SWS2s (λ max = 405-439 nm) but also the neighboring SWS1 (λ max = 356 nm) and RH2s (λ max = 452-516 nm) (Matsumoto et al, 2006). Indeed, we previously found that the wild-type and LWSa/b-knockout medaka could exhibit the OMR at λ ≤ 830 and ≤ 740 nm, respectively (Homma et al, 2017;Matsuo et al, 2018). That is, LWSs and RH2s can absorb and make medaka respond behaviorally to light at wavelengths > 200 nm longer than the λ max .…”

Section: Apparent Effects Of the Sws2 Mutations On Mate Choice But Nomentioning

confidence: 66%

“…As a lack of LWS clearly reduced sensitivity to red light (Homma et al, 2017;Matsuo et al, 2018), a lack of SWS2 may reduce sensitivity to blue light. Alternatively, the sensitivity may not be reduced because blue light could be absorbed by the neighboring violet and green opsins, whereas red (near infrared) light could not be absorbed by the neighboring green opsin.…”

Section: Behavioral Photosensitivity Of the Sws2 Mutantsmentioning

confidence: 99%

“…Colors represent genomic backgrounds of the strains [gray, color interfere (ci); orange, actin beta (Actb)-somatolactin alpha (SLα):green fluorescent protein (GFP)]. The OMR was quantified by three parameters: (A) delay: the time elapsed until the fish started the OMR after switching the direction of stripe rotation, (B) duration: the proportion of time the fish was exhibiting the OMR, and (C) distance: the distance the fish swam in the direction of stripe rotation during the test (Matsuo et al, 2018). Mean and standard error of the mean is shown as a box with a horizontal line.…”

Section: Adaptive Evolution Of Cone Opsin Repertoirementioning

confidence: 99%

See 3 more Smart Citations

Changes in a Cone Opsin Repertoire Affect Color-Dependent Social Behavior in Medaka but Not Behavioral Photosensitivity

et al. 2020

Self Cite

View full text Add to dashboard Cite

Common ancestors of vertebrates had four types of cone opsins: short-wavelength sensitive 1 (SWS1), SWS2, rhodopsin 2 (RH2), and long-wavelength sensitive (LWS) types. Whereas fish and birds retain all the types, mammals have lost two of them (SWS2 and RH2) possibly because of their nocturnal lifestyle during the Mesozoic Era. Considering that the loss of cone opsin types causes so-called color blindness in humans (e.g., protanopia), the ability to discriminate color by trichromatic humans could be lower than that in potentially tetrachromatic birds and fish. Behavioral studies using color-blind (cone opsin-knockout) animals would be helpful to address such questions, but it is only recently that the genome-editing technologies have opened up this pathway. Using medaka as a model, we introduced frameshift mutations in SWS2 (SWS2a and/or SWS2b) after detailed characterization of the loci in silico, which unveiled the existence of a GC-AG intron and non-optic expressed-sequence-tags (ESTs) that include SWS2a in part. Transcripts from the mutated SWS2 loci are commonly reduced, suggesting that the SWS2a/b-double mutants could produce, if any, severely truncated (likely dysfunctional) SWS2s in small amounts. The mutants exhibited weakened body color preferences during mate choice. However, the optomotor response (OMR) test under monochromatic light revealed that the mutants had no defect in spectral sensitivity, even at the absorbance maxima (λ max) of SWS2s. Evolutionary diversification of cone opsins has often been discussed in relation to adaptation to dominating light in habitats (i.e., changes in the repertoire or λ max are for increasing sensitivity to the dominating light). However, the present results seem to provide empirical evidence showing that acquiring or losing a type of cone opsin (or changes in λ max) need not

show abstract

Section: Apparent Effects Of the Sws2 Mutations On Mate Choice But Nocontrasting

confidence: 85%

Section: Apparent Effects Of the Sws2 Mutations On Mate Choice But Nomentioning

confidence: 66%

Section: Behavioral Photosensitivity Of the Sws2 Mutantsmentioning

confidence: 99%

Section: Adaptive Evolution Of Cone Opsin Repertoirementioning

confidence: 99%

See 2 more Smart Citations

Changes in a Cone Opsin Repertoire Affect Color-Dependent Social Behavior in Medaka but Not Behavioral Photosensitivity

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…In response to global, translational, whole-field motion, larval zebrafish will swim in the direction of perceived motion by generating swimming events called bouts. This reflex is known as the optomotor response (OMR) and can be elicited in both freely swimming and head-restrained zebrafish (Clark, 1981;Orger et al, 2000;Portugues and Engert, 2011), flies (Borst et al, 2010), mice (Matsuo et al, 2018;Shi et al, 2018), and many other species (Dieringer et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Optomotor Swimming in Larval Zebrafish Is Driven by Global Whole-Field Visual Motion and Local Light-Dark Transitions

Kist

Portugues

2019

Cell Reports

View full text Add to dashboard Cite

Graphical Abstract Highlights d Behavioral reverse correlation reveals the filter that drives optomotor swimming d The filter consists of a forward-moving local off edge and global whole-field motion d Light-dark transition is robustly found across both fish and stimuli parameters d Filter-specific neural activity is found across the zebrafish brain Authors Andreas M. Kist, Ruben Portugues Correspondence rportugues@neuro.mpg.de In BriefKist and Portugues use reverse correlation in an optomotor behavioral assay in larval zebrafish to identify the stereotypic filter that elicits swimming. It consists of a forward-moving local lightdark transition alongside global wholefield motion. The luminance profile strongly affects behavioral parameters, and filter-specific activity is spread across the brain. SUMMARYStabilizing gaze and position within an environment constitutes an important task for the nervous system of many animals. The optomotor response (OMR) is a reflexive behavior, present across many species, in which animals move in the direction of perceived whole-field visual motion, therefore stabilizing themselves with respect to the visual environment.Although the OMR has been extensively used to probe visuomotor neuronal circuitry, the exact visual cues that elicit the behavior remain unidentified. In this study, we use larval zebrafish to identify spatiotemporal visual features that robustly elicit forward OMR swimming. These cues consist of a local, forward-moving, off edge together with on/off symmetric, similarly directed, global motion. Imaging experiments reveal neural units specifically activated by the forward-moving light-dark transition. We conclude that the OMR is driven not just by wholefield motion but by the interplay between global and local visual stimuli, where the latter exhibits a strong light-dark asymmetry.

show abstract

The Opsin 3/Teleost multiple tissue opsin system: mRNA localization in the retina and brain of medaka ( Oryzias latipes )

2021

View full text Add to dashboard Cite

The photoreceptor protein, opsin, is one of the major components for vision and photoreceptive function in animals. Although many opsins have been discovered from animal genomes, only a few nonimage‐forming functions mediated by opsins have been identified. Understanding the mRNA distribution of photoreceptor proteins is one crucial step in uncovering their photoreceptive function in animals. Here, we focus on the medaka fish (Oryzias latipes) Opsin 3 (Opn3)/Teleost multiple opsin (Tmt) system, which constitutes a separate phylogenetic group, having putative blue light photoreceptors for nonimage‐forming functions. In medaka, there is one opn3 and five tmt‐opsin orthologs. The expression pattern of the opn3/tmt‐opsins in the retina and brain was investigated by in situ hybridization. mRNAs for opn3/tmt‐opsins were distributed in the retinal ganglion cells as well as interneurons and specific brain nuclei. Specifically, hybridization signals were observed in the glutamate decarboxylase 1 (gad1)‐expressing amacrine cells for opn3, tmt1a, tmt1b, and tmt2, in the caudal lobe of the cerebellum for tmt1b and tmt2, in the cranial nerve nuclei for opn3, tmt1a, tmt1b, tmt2, and in the rostral pars distalis (adenohypophysis) for opn3. These expression patterns suggest that blue light sensing in the fish retina and brain may be involved in the integration of visual inputs, vestibular function, somatosensation, motor outputs, and pituitary endocrine regulation.

show abstract

A semi-automatic and quantitative method to evaluate behavioral photosensitivity in animals based on the optomotor response (OMR)

Cited by 12 publications

References 31 publications

Changes in a Cone Opsin Repertoire Affect Color-Dependent Social Behavior in Medaka but Not Behavioral Photosensitivity

Changes in a Cone Opsin Repertoire Affect Color-Dependent Social Behavior in Medaka but Not Behavioral Photosensitivity

Optomotor Swimming in Larval Zebrafish Is Driven by Global Whole-Field Visual Motion and Local Light-Dark Transitions

The Opsin 3/Teleost multiple tissue opsin system: mRNA localization in the retina and brain of medaka ( Oryzias latipes )

Contact Info

Product

Resources

About