A semi-automatic, non-radioactive 384-well high throughput screening DIO2 assay

Frädrich, Caroline; Renko, Kostja; Wiese, Niklas; Fischbach, Anja; Seyffarth, Carola; Neuenschwander, Martin; von, Kries Jens Peter; Köhrle, Josef

doi:10.1530/endoabs.73.pep10.1

Cited by 2 publications

(3 citation statements)

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“…Automated pipetting systems are critical in the current rapid-development era, which can save time during detection or operation, reduce the risk of repetitive strain error from humans and ensure the reliability of pipetting results with high accuracy. They can also be used for special occasions that are not suitable for human involvement, such as with radioactive or infectious fluid (Allende et al, 2020;Frdrich et al, 2021;Kumar et al, 2021). As a result, increasing attention has been paid to highly automated micropump systems with the vast demand of the detection market, especially automated multichannel pipetting systems (Panackal et al, 2002;Quintanar-Melendez et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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An integrated low-cost automatic pipetting used in nucleic acid detection PCR instrument and clinical detection

Lin,

2024

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Purpose This paper aims to introduce a custom-designed integrated nucleic acid detection polymerase chain reaction (PCR) instrument for clinical detection applications. Design/methodology/approach The PCR instrument can make rapid, sensitive, low-cost and quantitative molecular diagnosis compared with the current routine test flow from the pipette, series reagent to RT-PCR by manual manipulation. By integrating the multichannel automatic pipetting module, heat amplification module and real-time fluorescence detection module for the first time, the custom-designed integrated nucleic acid detection PCR instrument can achieve sample collection, subpackage, mixing, extracting, measuring and result presentation. Findings The multichannel automatic pipetting module was assembled with an accuracy of 0.4% (2 microliters) for accuracy measurement. Besides, the accuracy and sensitivity of nucleic acid using integrated low-cost nucleic acid detection PCR instruments were checked with COV-2019 virus (staining method) and African swine fever virus (probe method) under different concentrations. Practical implications Because of its high cost, complex system and bulky laboratory settings, including sample subpackage, mixing, extracting, measuring and finally result in presentation, the current nucleic acid detection system is not suitable for field operation and disease diagnosis in remote areas. The group independently designed and assembled an integrated low-cost multichannel nucleic acid detection PCR instrument, including a multichannel automatic pipetting module, a heat amplification module and a real-time fluorescence detection module. Originality/value The above equipment showed better reliability compared with commercial qPCR. These results can lay the foundation for functional, fast and low-cost PCR equipment for trace measurements.

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Section: Introductionmentioning

confidence: 99%

“…They can also be used for special occasions that are not suitable for human involvement, such as with radioactive or infectious fluid (Allende et al. , 2020; Frdrich et al. , 2021; Kumar et al.…”

Section: Introductionmentioning

confidence: 99%

An integrated low-cost automatic pipetting used in nucleic acid detection PCR instrument and clinical detection

Lin,

2024

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“…Programmed mini-pumps are very important in the current rapid-development era, and can save much time during detection or operation, reduce the risk of repetitive strain error from humans, and ensure the reliability of pipetting results with high accuracy and can be used for special occasions, such as radioactive liquid, infectious fluid, or other environments not suitable for human operation [ 10 , 11 , 12 ]. As a result, with the huge demand of the detection market, more and more attention has been paid to highly automated pipetting systems.…”

Section: Introductionmentioning

confidence: 99%

Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR

et al. 2022

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Programmed mini-pumps play a significant role in various fields, such as chemistry, biology, and medicine, to transport a measured volume of liquid, especially in the current detection of (COVID-19) with PCR. In view of the cost of the current automatic pipetting pump being higher, which is difficult to use in a regular lab, this paper designed and assembled a three-dimensional programmed mini-pump with the common parts and components, such as PLC controller, motor, microinjector, etc. With the weighting calibration before and after pipetting operation, the error of the pipette in 10 μL (0.2%), 2 μL (1.8%), and 1 μL (5.6%) can be obtained. Besides, the contrast test between three-dimensional programmed mini-pump and manual pipette was conducted with the ORF1ab and pGEM-3Zf (+) genes in qPCR. The results proved that the custom-made three-dimensional programmed mini-pump has a stronger reproducibility compared with manual pipette (ORF1ab: 24.06 ± 0.33 vs. 23.50 ± 0.58, p = 0.1014; pGEM-3Zf (+): 11.83.06 ± 0.24 vs. 11.50 ± 0.34, p = 0.8779). These results can lay the foundation for the functional, fast, and low-cost programmed mini-pump in PCR or other applications for trace measurements.

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A semi-automatic, non-radioactive 384-well high throughput screening DIO2 assay

Cited by 2 publications

References 0 publications

An integrated low-cost automatic pipetting used in nucleic acid detection PCR instrument and clinical detection

An integrated low-cost automatic pipetting used in nucleic acid detection PCR instrument and clinical detection

Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR

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