A Semiselective Medium for the Isolation of<i>Pseudomonas syringae</i>pv.<i>savastanoi</i>

Surico, G.; Lavermicocca, Paola

doi:10.1094/phyto-79-185

Cited by 29 publications

(12 citation statements)

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“…These isolates were mostly nonfluorescent or produced very weak fluorescent colonies on KB or PVF‐1, as the fluorescence was lost after the first subculturing. On PVF‐1 medium, most of the isolates grew slowly, small, greyish‐white, slightly raised colonies with entire margins as reported previously (Surico and Lavermicocca ). The reactions of these bacterial isolates to Levan production, oxidase, potato soft rot and arginine dihydrolase tests were all negative.…”

Section: Resultssupporting

confidence: 82%

“…Letters in Applied Microbiology 59, 520--527 © 2014 The Society for Applied Microbiology (Surico and Lavermicocca 1989). The reactions of these bacterial isolates to Levan production, oxidase, potato soft rot and arginine dihydrolase tests were all negative.…”

Section: Resultsmentioning

confidence: 96%

“…Tenfold serial dilutions were spread on plates containing King's medium B (KB) (King et al . ) and PVF‐1 agar (Surico and Lavermicocca ) and incubated at 26°C for 3 days. Single fluorescent or nonfluorescent representative colonies of the predominant morphological types of bacterial isolates were re‐streaked onto new KB and PVF‐1 plates and incubated at 26°C for 3 days.…”

Section: Methodsmentioning

confidence: 99%

“…Small fragments (5-10 mm) from each knot were aseptically cut with a sterile scalpel and placed in a sterile eppendorf tube containing 1 ml sterile saline (0Á85% NaCl) and allowed to sit for 10 min. Tenfold serial dilutions were spread on plates containing King's medium B (KB) (King et al 1954) and PVF-1 agar (Surico and Lavermicocca 1989) and incubated at 26°C for 3 days. Single fluorescent or nonfluorescent representative colonies of the predominant morphological types of bacterial isolates were re-streaked onto new KB and PVF-1 plates and incubated at 26°C for 3 days.…”

Section: Isolation Of Bacterial Isolatesmentioning

confidence: 99%

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Characterization of bacterial knot disease caused by Pseudomonas savastanoi pv. savastanoi on pomegranate (Punica granatum L.) trees: a new host of the pathogen

Bozkurt

Soylu

Mırık

et al. 2014

Lett Appl Microbiol

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Pomegranate trees were affected by the disease with outgrowths (galls or knot) disease. Currently, there is no published study on disease agent(s) causing the galls or knots on pomegranate trees in worldwide. Bacterial colonies were isolated from young knots. The causal agent of the knot Pseudomonas savastanoi pv.savastanoi (Psv) was identified based on symptoms, biochemical, molecular methods, pathogenicity tests and sequence analysis. To the best of our knowledge, this is the first report of Psv on pomegranate as a natural host, which extends the growing list of plant species affected by this bacterium in the world and Turkey.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Isolation Of Bacterial Isolatesmentioning

confidence: 99%

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Characterization of bacterial knot disease caused by Pseudomonas savastanoi pv. savastanoi on pomegranate (Punica granatum L.) trees: a new host of the pathogen

Bozkurt

Soylu

Mırık

et al. 2014

Lett Appl Microbiol

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“…Pantoea agglomerans has often been reported to be associated with olive and oleander knots (Surico & Lavermicocca, 1989; Azad & Cooksey, 1995; Fernandes & Marcelo, 2002). The bacterium is considered an epiphyte and is very common on the aerial parts of plants (Starr & Chatterjee, 1972), including disease lesions (Gibbins, 1978).…”

Section: Introductionmentioning

confidence: 99%

Interaction between Pseudomonas savastanoi pv. savastanoi and Pantoea agglomerans in olive knots

et al. 2006

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Results of a survey of olive knot disease in central Italy in 2002 and 2003 showed that Pantoea agglomerans was found associated with the pathogen Pseudomonas savastanoi pv. savastanoi ( Ps. savastanoi ) in 70% of the olive knots examined. Pathogenicity tests in which these two bacteria were co-inoculated on the stems of 1-year-old olive plants at ratios of 1:1, 1:100 and 100:1 showed that the growth of P. agglomerans was apparently aided by the presence of an actively growing population of Ps. savastanoi . At the same time, however, a dominant population of P. agglomerans at the inoculation site tended to depress the growth of Ps. savastanoi , probably because of competition for space and nutrients between these bacteria and by means of antibiotic production by P. agglomerans. In some cases the association of P. agglomerans , which in culture was found to produce indole-3-acetic acid but not cytokinins, with Ps. savastanoi resulted in an increase in the size of knots. This boosting effect of P. agglomerans on proliferation was probably due to the release of IAA by this bacterium at the inoculation sites.

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